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EC number: 944-090-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From October 4, 2007 to November 1, 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 306 (Biodegradability in Seawater)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- natural water: marine
- Remarks:
- Biologically active
- Details on inoculum:
- - Source of inoculum: Biologically active natural seawater collected from Anglesey Sea Zoo in North Wales on September 5, 2007
- Storage conditions: Maintained in the dark prior to use
- Storage length: ~4 weeks
- Preparation of inoculum for exposure: Seawater was aged prior to use by gentle aeration at 15 ± 2 °C
- Water filtered: After collection the seawater was coarse filtered and then maintained in the dark
INOCULUM PROPERTIES (at collection)
- Temperature: 17.0 °C (in the study all bottles were incubated at 17.5 - 20.5 °C)
- Salinity: 31.4 g/l
- pH: 7.64
- Dissolved oxygen: 100.0 % - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2.5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 3 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Remarks:
- i.e. Dissolved oxygen (DO), used to derive % biodegradability in conjunction with calculated chemical oxygen demand (COD)
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Natural, nutrient fortified seawater that had been coarse filtered. Test medium was prepared according to the method detailed in STL Runcorn SOP III.36
- Test temperature: All bottles were incubated at 17.5 - 20.5 °C
- pH: 7.64 at point of collection of seawater.
- Aeration of dilution water: Seawater received gentle aeration prior to use
- Continuous darkness: Yes, all bottles were incubated in the dark
TEST SYSTEM
- Culturing apparatus: Sealed glass BOD bottles of nominal 272 ml volume
- Number of culture flasks/concentration: Duplicate bottles were prepared for each of the sample days, for each test concentration of 2.5 mg/l and 3.0 mg/l test substance, and 2.5 mg/l sodium benzoate (a reference material to monitor microbial activity). Three replicates were prepared for use as control vessels for each day, which contained nutrient fortified seawater only.
- Method used to create aerobic conditions: Gentle aeration of seawater prior to test use
- Test performed in closed/open vessels: Closed bottle procedure
SAMPLING
- Sampling frequency: Days 0, 3, 7, 14, 21, and 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes, nutrient fortified seawater only (controls)
STATISTICAL METHODS:
The calculated COD value and dissolved oxygen data recorded at each analysis point (including day 0 readings) were processed to derive the percentage (%) degradability of the test material. Degradation values were calculated using the equation:
% Degradability = (BOD mgO2/mg test material / COD (mgO2/mg)) x 100 - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 2.5 mg/l
- Test performance:
- No deviation from the guideline was reported
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- 2.5 mg/l p-toluene sulphonamidocaproic acid morpholine salt
- Value:
- 26.2
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- 3.0 mg/l p-toluene sulphonamidocaproic acid morpholine salt
- Value:
- 19.5
- Sampling time:
- 28 d
- Details on results:
- At 2.5 mg/l and 3.0 mg/l of p-toluene sulphonamidocaproic acid morpholine salt, % biodegradability was reported to be 26.2 and 19.5 % respectively after 28 days. This would suggest that the test substance has a limited potential for degradation under these experimental conditions.
- Key result
- Parameter:
- COD
- Value:
- 1.571 other: mg/mg
- Remarks on result:
- other: Calculated with COD equation in STL Runcorn SOP III.1
- Results with reference substance:
- After day 28, sodium benzoate (2.5 mg/l) had a % biodegradability of 71.9 %. The inoculum was considered to be biologically active.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- By the end of the experiment on Day 28, the test substance at 2.5 and 3.0 mg/l had been biodegraded by 26.2 and 19.5 % (5.81 and 5.87 mgO2/L), respectively. It can be concluded that the substance is not ready biodegradable.
- Executive summary:
The ready biodegradation of the test substance was determined in a biological oxygen demand (BOD), GLP-certified test undertaken in accordance with OECD Guideline 306 (Biodegradability in Seawater).
The aim of the OECD 306 test is to establish the biodegradability of an organic material in a marine environment. Natural seawater considered to be biologically active was acquired from North Wales and nutrient fortified for use as the study inoculum. The registration substance at 2.5 and 3.0 mg/l, sodium benzoate at 2.5 mg/l (as a reference material to monitor microbial activity), and a seawater only blank were maintained at 17.5 - 20.5 °C in the dark and reviewed on day 0, 3, 7, 14, 21, and 28. Measurement for oxygen was taken in the form of DOC at the time intervals specified and COD calculated. DOC and COD were used to determine percentage (%) biodegradability of the test material.
The % biodegradation of sodium benzoate was reported to be 71.9 % over the 28-day study duration, indicating a biologically active inoculum, with a DOC of 3.83 mgO2/L. COD for the test substance was found to be 1.571 mg/mg.
By the end of the experiment on Day 28, the test substance at 2.5 and 3.0 mg/l had been biodegraded by 26.2 and 19.5% (5.81 and 5.87 mgO2/L), respectively. It can be concluded that the substance is not ready biodegradable.
Reference
Summary Table
Material (mg/l) | % Degradability | ||||
Day 3 | Day 7 | Day 14 | Day 21 | Day 28 | |
p-Toluene sulphonamidocaproic acid morpholine salt (2.5) | * | 1.5 | 12.5 | 10.2 | 26.2 |
p-Toluene sulphonamidocaproic acid morpholine salt (3.0) | 0.4 | 3.0 | 32.3 | 7.6 | 19.5 |
Sodium benzoate (2.5)** | 48.9 | 74.7 | 46.5 | 61.3 | 71.9 |
**Using the calculated theoretical oxygen demand (ThOD) of sodium benzoate as 1.67 mgO2/mg
Description of key information
By the end of the experiment on Day 28, the test substance at 2.5 and 3.0 mg/l had been biodegraded by 26.2 and 19.5 % (5.81 and 5.87 mgO2/L), respectively. It can be concluded that the substance is not ready biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- marine water
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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