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EC number: 223-989-2 | CAS number: 4156-21-2
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
In this study Active Substance of Granofin Easy F-90 liq in Aqueous Solution was found to cause no toxic effects to Zebrafish after 96 hours when tested with the limit concentration of 143 mg test item/L, corresponding to 100 mg main component/L.
The LC50 after 96 hours is > 143 mg test item/L. All effect levels are given based on the nominal concentration level of the test item.
Key value for chemical safety assessment
Additional information
The acute toxicity of the test item Active substance of Granofin Easy F-90 liq in aqueous solution to fish (zebrafish) was determined according to OECD-Guideline for Testing of Chemicals No. 203 (1992) from 2012-03-12 to 2012-03-30 with the definite exposure phase from 2012-03-26 to 2012-03-30, atDr.U.Noack-Laboratorien, 31157 Sarstedt, Germany. A static test with one limit concentration of nominal 143 mg test item/L, corresponding to 100 mg main component/L, was performed.
The duration of the test was 96 h. Seven test organisms were exposed to the limit concentration and the control. Water quality parameters pH-value, temperature and oxygen-saturation measured after 0, 24, 48, 72 and 96 h were determined to be within the acceptable limits.
The determination of the test item Active substance of Granofin Easy F-90 liq in aqueous solution was carried out via HPLC-DAD from the limit concentration and the control at test start and test end. The recovery rates of Active substance of Granofin Easy F-90 liq in aqueous solution at start and end of the test were 103 % and 92 % of the nominal value, respectively. For details of the analytical results please refer to part 8. All effect levels are given based on the nominal concentration of the test item Active substance of Granofin Easy F-90 liq in aqueous solution (Table 1).
LC-Values with 95 % Confidence Intervals
based on nominal test item concentration [mg/L]
Test duration [h] |
LC50 |
p = 95 % |
24 |
> 143 |
n.a. |
48 |
> 143 |
n.a. |
72 |
> 143 |
n.a. |
96 |
> 143 |
n.a. |
LC100= |
> 143 |
|
Lowest test item concentration |
||
with 100 % mortality after 96 h |
||
LC0= |
143 |
|
Highest test item concentration |
||
with 0 % mortality after 96 h |
||
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