p-anisylamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

of read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of fish for the test chemical :
1 and 2 . This study was designed to assess the toxic effects of the test compound on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203.

Specific details on test material used for the study:

- Name of test material: 4-Methoxybenzylamine
- IUPAC name: p-anisylamine
- Molecular formula: C8H11NO
- Molecular weight: 137.181 g/mole
- Smiles :O(c1ccc(cc1)CN)C
- Inchl: 1S/C8H11NO/c1-10-8-4-2-7(6-9)3-5-8/h2-5H,6,9H2,1H3
- Substance type: Organic
- Physical state: Liquid (Colorless to pale yellow)

Details on sampling:

1.The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L, respectively.

Vehicle:

not specified

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

1 , 2 :TEST ORGANISM
- Animal species: Zebra fish(Danio rerio)
- Animal breeder/ supplier: Orange City Aquarium, Sitabuldi, Nagpur, MS (India)
- Body length: average: 2.5 cm
- Body weight: 0.162 g (average)

The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
- Duration of housing: 15 days
- Photoperiod: 16 hours light and 8 hours dark
- Oxygen content: 5.94 (mg/l)
- pH: 5.7
- Water temperature: 30°C
- Mortality (%): During last 14 days 0 mortality was observed
- Diet: Standard brand feed, once daily

- Acclimatization: Fishes, to be used in the study, are kept in the test water for seven days immediately before testing under following conditions:
- Photoperiod: 16 hours light and 8 hours dark
- Temperature range: 23 °C
- pH range: 5.77
- DO range: 5.94 mg/l
- Feeding: Daily once
- Aeration: Continuous

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

24, 48, 72, 96 hrs

Hardness:

1 - 50 (mg of CaCO3)
2 - 148.5 mg of CaCO3

Test temperature:

22-23°C

pH:

5.7-7.05

Dissolved oxygen:

1 - 5.94(mg/l)
2 - 8.0 mg/l

Nominal and measured concentrations:

100 mg/l

Details on test conditions:

1.TEST SYSTEM
- Test vessel: Plastic aquaria
- Type (delete if not applicable): open / closed: No data
- Material, size, headspace, fill volume: Plastic aquaria having the water capacity of 5 liters filled with 4 liters of water with 1 liter of headspace.
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
- Withdrawal of food: 1 day before and during exposure

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

- EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment.

2.TEST SYSTEM
- Test vessel:The test vessels used were all plastic aquaria having the water capacity of 5liters.
- Material, size, headspace, fill volume: 2 liters
- Aeration: No
- No. of organisms per vessel:8

OTHER TEST CONDITIONS
- Adjustment of pH:No
- Photoperiod:16 hours light and 8 hours dark

TEST CONCENTRATIONS
- Test concentrations: 0,100 mg/l

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Read across 1

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Read across 2

Details on results:

2.A 96 h study was conducted for on Zebra fish(Danio rerio), at a test concentrations of 100mg/L . The fishes were moving slowly as compared to control.

Conclusions:

The test chemical 4-Methoxybenzylamine is not likely to be toxic to fish in concentration > 100 mg/l

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 4-Methoxybenzylamine (2393 -23 -9).The studies are as mentioned below:

1.This study was designed to assess the toxic effects of the test compound 4-Methoxy benzyl alcohol on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L, respectively.

 

Test conducted at limit test concentration 100 mg/l and Zebra fishes were exposed to this concentration for 96 hours. Test animal was collected from Orange City Aquarium, Sitabuldi, Nagpur, MS (India). 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment.  

 

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical on Zebra fish (Danio rerio) was determined to be > 100 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

2. Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical. The nominal concentration selected for the experiment were 100mg/L and test fish were exposed to these concentration for 96 hours. The median lethal concentration (LC50) for test chemical on Danio rerio in a 96 hours study on the basis of mortality effect was found to be >100 mg/L. Thus, on the basis of this LC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, test chemical does not exhibit short term toxicity to fish. LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L .LC50 (96 hours) Experimental = >100 mg/L

Thus, based on the above summarised studies, 4-Methoxybenzylamine and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is >100 mg/l. Thus, comparing this value with the criteria of CLP regulation 4-Methoxybenzylamine cannot be classified

for short term toxicity for fish .Hence,based on the data available for the structurally and functionally similarread across, test chemical 4-Methoxybenzylamine is not likely to be toxic atleast in the concentration >100 mg/L .

Description of key information

Short-term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 4-Methoxybenzylamine (2393 -23 -9).The studies are as mentioned below:

1.This study was designed to assess the toxic effects of the test compound 4-Methoxy benzyl alcohol on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L, respectively.

 

Test conducted at limit test concentration 100 mg/l and Zebra fishes were exposed to this concentration for 96 hours. Test animal was collected from Orange City Aquarium, Sitabuldi, Nagpur, MS (India). 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment.  

 

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical on Zebra fish (Danio rerio) was determined to be > 100 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

2.Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical. The nominal concentration selected for the experiment were 100mg/L and test fish were exposed to these concentration for 96 hours. The median lethal concentration (LC50) for test chemical on Danio rerio in a 96 hours study on the basis of mortality effect was found to be >100 mg/L. Thus, on the basis of this LC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, test chemical does not exhibit short term toxicity to fish. LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L .LC50 (96 hours) Experimental = >100 mg/L

Thus, based on the above summarised studies, 4-Methoxybenzylamine and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is >100 mg/l. Thus, comparing this value with the criteria of CLP regulation 4-Methoxybenzylamine cannot be classified

for short term toxicity for fish .Hence,based on the data available for the structurally and functionally similarread across, test chemical 4-Methoxybenzylamine is not likely to be toxic atleast in the concentration >100 mg/L .

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Short-term toxicity to fish:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of fish of the test chemical 4-Methoxybenzylamine (2393 -23 -9).The studies are as mentioned below:

1.This study was designed to assess the toxic effects of the test compound 4-Methoxy benzyl alcohol on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L, respectively.

 

Test conducted at limit test concentration 100 mg/l and Zebra fishes were exposed to this concentration for 96 hours. Test animal was collected from Orange City Aquarium, Sitabuldi, Nagpur, MS (India). 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment.  

 

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical on Zebra fish (Danio rerio) was determined to be > 100 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

2.Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical. The nominal concentration selected for the experiment were 100mg/L and test fish were exposed to these concentration for 96 hours. The median lethal concentration (LC50) for test chemical on Danio rerio in a 96 hours study on the basis of mortality effect was found to be >100 mg/L. Thus, on the basis of this LC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, test chemical does not exhibit short term toxicity to fish. LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L .LC50 (96 hours) Experimental = >100 mg/L

Thus, based on the above summarised studies, 4-Methoxybenzylamine and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is >100 mg/l. Thus, comparing this value with the criteria of CLP regulation 4-Methoxybenzylamine cannot be classified

for short term toxicity for fish .Hence,based on the data available for the structurally and functionally similarread across, test chemical 4-Methoxybenzylamine is not likely to be toxic atleast in the concentration >100 mg/L .