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Toxicological information

Direct observations: clinical cases, poisoning incidents and other

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Administrative data

Endpoint:
direct observations: clinical cases, poisoning incidents and other
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1992/93
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Average chlorobenzene concentration and air concentrations of benzene, chloroform, methanol and toluene were also given.

Data source

Reference
Title:
No information
Author:
Major J et al (1992). Acta Medica Hungarica, 49:79-90.

Materials and methods

Study type:
other: study on workers exposed to chlorobenzen air concentrations of benzene, chloroform, methanol and toluene
Endpoint addressed:
genetic toxicity
Test guideline
Qualifier:
equivalent or similar to
Guideline:
other: OECD guideline 476
Deviations:
yes
Remarks:
Test performed on human lymphocytes. No postive control used
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Average chlorobenzene concentration was 33.5 +- 17.77 mg/m3 air (range 1.9 - 173.4 mg/m3 air), air concentrations of benzene, chloroform, methanol and toluene were also given.

Method

Subjects:
workers
Ethical approval:
not specified
Route of exposure:
inhalation
Reason of exposure:
other: exposure at workplace
Exposure assessment:
estimated
Details on exposure:
exposure at workplace:Average chlorobenzene concentration was 33.5 +- 17.77 mg/m3 air (range 1.9 - 173.4 mg/m3 air), air concentrations of benzene, chloroform, methanol and toluene were also given.

Results and discussion

Clinical signs:
No data

Any other information on results incl. tables

Chronic effects:
A possible genotoxic effect was assessed in workers
manufacturing the pesticide Lutidin of monochlorinated
benzene. Average chlorobenzene concentration was 33.5 +-
17.77 mg/m3 air (range 1.9 - 173.4 mg/m3 air), air
concentrations of benzene, chloroform, methanol and toluene
were also given. The urinary phenol concentration of exposed
workers was 602.3 +- 89.3 mg/l (n = 18). Peripheral blood
lymphocytes were obtained from 147 exposed workers, 33
industrial control workers and 60 historical controls and
assessed for mutations in the hypoxanthine - (guanine) -
phosphoribosyl transferase (HGPRT) locus of the X chromosome
by measuring the 6-thioguanine (6-TG) resistance and for DNA
repair capacity by measuring the unscheduled DNA synthesis
after 250 nm UV light irradiation. Mutation frequencies were
increased among workers exposed to monochlorinated benzene
in correlation with the duration of work time, compared to
the controls. Mutation frequencies were lower than expected
among non-smoking, long exposed workers (> 10 years).
Smoking itself proved to be an effective confounding factor
in the enhancement of point mutations in the case of
long-exposed workers. DNA repair capacity of exposed workers
was not significantly increased compared to industrial
control.
(Hungary; published 1992/93)

Applicant's summary and conclusion