Chlorobenzene

Direct observations: clinical cases, poisoning incidents and other

Administrative data

Endpoint:

direct observations: clinical cases, poisoning incidents and other

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1992/93

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Average chlorobenzene concentration and air concentrations of benzene, chloroform, methanol and toluene were also given.

Data source

Reference

Title:

No information

Author:

Major J et al (1992). Acta Medica Hungarica, 49:79-90.

Materials and methods

Study type:

other: study on workers exposed to chlorobenzen air concentrations of benzene, chloroform, methanol and toluene

Endpoint addressed:

genetic toxicity

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: OECD guideline 476

Deviations:

yes

Remarks:

Test performed on human lymphocytes. No postive control used

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Chlorobenzene

EC Number:

203-628-5

EC Name:

Chlorobenzene

Cas Number:

108-90-7

Molecular formula:

C6H5Cl

IUPAC Name:

chlorobenzene

Details on test material:

Average chlorobenzene concentration was 33.5 +- 17.77 mg/m3 air (range 1.9 - 173.4 mg/m3 air), air concentrations of benzene, chloroform, methanol and toluene were also given.

Method

Subjects:

workers

Ethical approval:

not specified

Route of exposure:

inhalation

Reason of exposure:

other: exposure at workplace

Exposure assessment:

estimated

Details on exposure:

exposure at workplace:Average chlorobenzene concentration was 33.5 +- 17.77 mg/m3 air (range 1.9 - 173.4 mg/m3 air), air concentrations of benzene, chloroform, methanol and toluene were also given.

Results and discussion

Clinical signs:

No data

Any other information on results incl. tables

Chronic effects:
A possible genotoxic effect was assessed in workers
manufacturing the pesticide Lutidin of monochlorinated
benzene. Average chlorobenzene concentration was 33.5 +-
17.77 mg/m3 air (range 1.9 - 173.4 mg/m3 air), air
concentrations of benzene, chloroform, methanol and toluene
were also given. The urinary phenol concentration of exposed
workers was 602.3 +- 89.3 mg/l (n = 18). Peripheral blood
lymphocytes were obtained from 147 exposed workers, 33
industrial control workers and 60 historical controls and
assessed for mutations in the hypoxanthine - (guanine) -
phosphoribosyl transferase (HGPRT) locus of the X chromosome
by measuring the 6-thioguanine (6-TG) resistance and for DNA
repair capacity by measuring the unscheduled DNA synthesis
after 250 nm UV light irradiation. Mutation frequencies were
increased among workers exposed to monochlorinated benzene
in correlation with the duration of work time, compared to
the controls. Mutation frequencies were lower than expected
among non-smoking, long exposed workers (> 10 years).
Smoking itself proved to be an effective confounding factor
in the enhancement of point mutations in the case of
long-exposed workers. DNA repair capacity of exposed workers
was not significantly increased compared to industrial
control.
(Hungary; published 1992/93)