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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The ready biodegradability of the test substance was examined according to the OECD 301 B (CO2 evolution, modified Sturm test). The degree of biodegradation after an exposure period of 28 days was 10-20 % CO2/ThCO2 in this test. The test substance was found to be not readily biodegradable under the test conditions. 

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The ready biodegradability of the test substance was examined in a test (BASF SE, 22G0476/14G402, 2016) according to the OECD 301 B (CO2 evolution, modified Sturm test, 1992). The biodegradation of the test substance was followed by exposing it to the activated sludge from a municipal sewage plant (Municipal waste water treatment plant Mannheim, Germany). The activated sludge was aerated with carbon dioxide free air for about 48 hours at 22 ± 2 °C. The activated sludge concentration was 30 mg/L (dry weight). The concentration of the test substance was 31.5 mg/L (corresponding to 20 mg/L TOC). As a reference compound (procedure control), aniline was tested simultaneously under the same conditions. No inhibitory effect on the microorganisms was observed. The degree of biodegradation of the reference substance after 14 days was determined to be 88 % CO2/ThCO2 and the degree of biodegradation in the inhibition control was determined to be 45 % CO2/ThCO2 after 14 days. The validity criteria were met. The test substance was degraded 10-20 % CO2/ThCO2 at the end of the exposure. The test substance was found to be not readily biodegradable under the test conditions.