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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

Currently viewing:

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed publication

Data source

Reference
Reference Type:
publication
Title:
Comparisons of mutation induction in reversion systems of Saccharomyces Cerevisiae and Salmonella Typhimurium
Author:
Majdi m. shahin and R.C.Von borstel
Year:
1978
Bibliographic source:
Mutation Research, 53 (1978) 1—10

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other:
Principles of method if other than guideline:
To evaluate the genotoxicity of methyl violet in Salmonella typhimurium strain TA1535, TA100, TA1537, TA1538 and TA98.
GLP compliance:
not specified
Type of assay:
bacterial gene mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
8004-87-3
EC Number:
616-846-4
Cas Number:
8004-87-3
Molecular formula:
C24H28ClN3
IUPAC Name:
8004-87-3
Constituent 2
Reference substance name:
C.I. Basic Violet 1
IUPAC Name:
C.I. Basic Violet 1
Details on test material:
- Name of test material (as cited in study report): Methyl violet
- Molecular formula (if other than submission substance): C24H28ClN3
- Molecular weight (if other than submission substance): 393.95 g/mol
- Substance type: Organic
- Physical state: Dark yellow-green to dark green crystal (nub)
Purity- No data available

Method

Target gene:
No data
Species / strain
Species / strain / cell type:
S. typhimurium, other: TA1535, TA100, TA1537, TA1538 and TA98
Details on mammalian cell type (if applicable):
Not applicable
Additional strain / cell type characteristics:
other: strains are excision-repair deficient and contain the gal and rfa mutations.
Metabolic activation:
not specified
Metabolic activation system:
No data
Test concentrations with justification for top dose:
1, 2 or 4µg/plate
Vehicle / solvent:
No data available
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified
Details on test system and experimental conditions:
METHOD OF APPLICATION: Top agar method was used. {soft agar procedure}
Evaluation criteria:
Revertant colonies per plate were observed.
Statistics:
No data available

Results and discussion

Test results
Species / strain:
S. typhimurium, other: TA1535, TA100, TA1537, TA1538 and TA98
Metabolic activation:
not specified
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
No data available
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

The test result was observed to be negative for Methyl violet in Salmonella typhimurium Strain- TA1535, TA100, TA1537, TA1538 and TA98 by soft agar procedure.
Executive summary:

Genotoxicity study for Methyl violet (CAS no 8004 -87 -3) was conducted in Salmonella typhimurium strain -TA1535, TA100, TA1537, TA1538 and TA98 bysoft agar procedure. They were exposed at the concentration of1, 2, and 4µg\plate. Revertant colonies per plate were observed. The test result was observed to be negative for Methyl violet in Salmonella typhimurium Strain-TA1535, TA100, TA1537, TA1538 and TA98. Based on the above result ,it can be summarized that Methyl violet is not likely to exhibit genetic toxicity and hence can be classified as 'non-hazardous'.