Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20.09.2016 - 23.09.2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Experimental test result performed using standard test guideline

Justification for type of information:

Data is from ABITEC lab report.

Principles of method if other than guideline:

Short term toxicity of Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides to aquatic algae was performed according to the OECD guideline in a static system.

GLP compliance:

not specified

Specific details on test material used for the study:

- Common name: Basic violet
- IUPAC name: Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

The stock solution 10 mg/l was prepared by dissolving dark violet powder in OECD growth medium. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name:
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available

ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Remarks on exposure duration:

±1 hour

Test temperature:

23±2°C

pH:

Test at highest concentration 0.68 mg/l: 7.8 (Change to 7.7 during tests)
Control: 8.3 (changed to 7.8 during test)

Nominal and measured concentrations:

0, 0.13, 0.20, 0.30, 0.45, 0.68 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 7.5 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Key result

Duration:

72 h

Dose descriptor:

other: ErC50

Effect conc.:

0.33 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95% CI was 0.22 - 0.49 mg/l

Results with reference substance (positive control):

Results with reference substance valid
- ErC50: 0.69 mg/L

Reported statistics and error estimates:

ErC50 is calculated from the inhibition of growth rate by non-linear regression using software Prism 4.0, GraphPad.

Validity criteria fulfilled:

not specified

Conclusions:

The median effective concentration (ErC50) for the test substance Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides, in Desmodesmus subspicatus was determined to be 0.33 mg/L on the basis of effects on growth rate in a 72 hour study.

Executive summary:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides (83968 -28 -9). Test was conducted according to OECD Guideline 201.

The stock solution 10 mg/l was prepared by dissolving dark violet powder in OECD growth medium. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and tested at the concentrations 0, 0.13, 0.20, 0.30, 0.45, 0.68 mg/l. Effects on the growth rate of the organism were studied. Apart from test chemical, positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. Test conducted in 50 ml glass vessel filled with 7.5 ml of test solution and performed in 3 replicates. After the exposure of chemical for 72 hrs, ErC50 was calculated using non linear regression by the software Prism 4.0.

The median effective concentration (ErC50) for the test substance Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides, in Desmodesmus subspicatus was determined to be 0.33 mg/L.

This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic acute/chronic 1 category as per the CLP criteria.

Description of key information

The median effective concentration (ErC50) for the test substance Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides, in Desmodesmus subspicatus was determined to be 0.33 mg/L. This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic acute/chronic 1 category as per the CLP criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:

0.33 mg/L

Additional information

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides (83968 -28 -9). Test was conducted according to OECD Guideline 201.

The stock solution 10 mg/l was prepared by dissolving dark violet powder in OECD growth medium. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and tested at the concentrations 0, 0.13, 0.20, 0.30, 0.45, 0.68 mg/l. Effects on the growth rate of the organism were studied. Apart from test chemical, positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. Test conducted in 50 ml glass vessel filled with 7.5 ml of test solution and performed in 3 replicates. After the exposure of chemical for 72 hrs, ErC50 was calculated using non linear regression by the software Prism 4.0.

The median effective concentration (ErC50) for the test substance Formaldehyde, reaction products with N,N-dimethylbenzenamine and N-methylbenzenamine, oxidized, hydrochlorides, in Desmodesmus subspicatus was determined to be 0.33 mg/L. This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic acute/chronic 1 category as per the CLP criteria.