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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
April 1977
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1978
Report Date:
1978
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report Date:
1978

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Five male and five female mice were treated with a single intraperitoneal dose of test material at a dose level of 2000 mg/kg followed by a 14 day observation period.
GLP compliance:
no
Limit test:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
mouse
Strain:
CD-1
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Charles River (U.K.) Ltd., Manston Road, Margate, Kent
- Weight at study initiation: on the day before dosing individual body weights were in the range 17 - 24 g.
- Fasting period before study: overnight fast (for 18 - 20 hours before treatment)
- Housing: solid floor boxes furnished with sterilised sawdust, in groups of 5
- Diet (e.g. ad libitum): Rat and Mause No. 1 Expanded Diet (B.P. Nutrition (U.K.) Ltd., Stepfield, Witham, Essex), ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: for a minimum of 3 days.

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 3
- Humidity (%): 50 ± 10
- Air changes (per hr): animais were housed in a single air-conditioned room
- Photoperiod (hrs dark / hrs light): natural lighting conditions

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
water
Doses:
2000 mg/kg
No. of animals per sex per dose:
5
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Animais were observed for overt toxicity and mortality at 15 minutes and 1, 2 and 4 hours after treatment and subsequently once daily for 14 days and the observations were recorded. Body weights of survivors were recorded 14 days after treatment.
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Based on:
test mat.
Remarks on result:
other: mortality was observed
Mortality:
Two female mice died within 24 hours of treatment.
Clinical signs:
No signs of overt toxicity were apparent during the 14 day observation period.
Body weight:
All surviving animals showed normal body weight gains throughout the observation period.

Applicant's summary and conclusion

Conclusions:
The test substance was investigated for its acute toxic potential. Two out of five animals died within 24 h of treatment. The LD50 was concluded to be greater than 2000 mg/kg bw.