Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Principles of method if other than guideline:
according to internal standard method
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,9-bis(2-phenylethyl)anthra[2,1,9-def:6,5,10-d'e'f']diisoquinoline-1,3,8,10(2H,9H)-tetrone
EC Number:
266-564-7
EC Name:
2,9-bis(2-phenylethyl)anthra[2,1,9-def:6,5,10-d'e'f']diisoquinoline-1,3,8,10(2H,9H)-tetrone
Cas Number:
67075-37-0
Molecular formula:
C40H26N2O4
IUPAC Name:
7,18-bis(2-phenylethyl)-7,18-diazaheptacyclo[14.6.2.2²,⁵.0³,¹².0⁴,⁹.0¹³,²³.0²⁰,²⁴]hexacosa-1(23),2,4,9,11,13,15,20(24),21,25-decaene-6,8,17,19-tetrone
Details on test material:
Test materials used in this dossier are all considered to fall under the definition of nano-materials according to the European Commission Recommendation 2011/696/EU as the synthesis and manufacturing of this pigment always yields particulate material with a fine particle size distribution.

Test animals

Species:
mouse
Strain:
other: NMRI-Wiga
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: males 26.0 g, females 24.0 g
- Diet (e.g. ad libitum): HERTLAN MRH Haltung, Alleinfutter für die Haltung von Mäusen, Ratten unrl Hamstern, Heinrich EGGERSMANN KG, Rinteln.

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
CMC (carboxymethyl cellulose)
Remarks:
0.5% in water
Details on exposure:
The test substance was given as a 20 or 50% suspension with a 0.5% aqueous carboxymethyl cellulose solution
Doses:
2000 mg/kg
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: daily (except on weekends)
- Frequency of weighing: before treatment and on days 2, 7 and 13
- Necropsy of survivors performed: yes
- Other examinations performed: clinical, body weight

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Based on:
test mat.
Remarks on result:
other: no mortality observed
Mortality:
None of the animals died during the exposure period.
Clinical signs:
At the first observation day a deteriorated general health. Symptoms were dyspnoea, apathy, staggering and unsteady gait
Body weight:
Initially weight loss.
Day 2, mean weight (g): males: 25.2; females 22.0
Day 7, mean weight (g): males: 30.8; females 25.7
Day 13, mean weight (g): males: 32.8; females 27.3
Gross pathology:
Intra-abdominal substance incorporation.

Applicant's summary and conclusion