Soybean oil, epoxidised, reaction products with methanol and water

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: OECD guideline conform

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

test concentration above water solubility

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Soybean oil, epoxidized

EC Number:

232-391-0

EC Name:

Soybean oil, epoxidized

Cas Number:

8013-07-8

IUPAC Name:

8013-07-8

Details on test material:

The test material is insoluble in water. A stock solution was prepared: 4.0 g of test material were dissolved in and made up to 10 ml with DMF. This solution was diluted to 100 mg/l with water.
Concentrations Nominal: The calculated amounts of stock solution to produce the desired test concentrations were given into the water and were homogeneously distributed. The daphnia were then transferred into the beakers.

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

- Concentrations: 10, 18, 32, 58 and 100 mg/l.

Test solutions

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)

- Evidence of undissolved material (e.g. precipitate, surface film, etc): Small parts of the test substance were swimming at the surface of the test solutions at concentrations 10-100 mg/l (nominal) from the start of the test

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

not specified

Water media type:

not specified

Limit test:

no

Total exposure duration:

24 h

Test conditions

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

> 100 mg/L

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Basis for effect:

mobility

Details on results:

No immobilization was noted in any treated organism.

Applicant's summary and conclusion

Conclusions:

Test concentrations were well above the limit of solubility. These test conditions may be considered to represent a worst-case exposure condition, as the possibility for both direct toxicity (narcosis) of dissolved substance and solvent vehicle, and indirect toxicity (physical effects) of undissolved test substance were represented.
Regardless of this potential for multiple modes of toxicity, acute EC50 value indicates the substance to be practically non-toxic to crustaceae on an acute basis.