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EC number: 620-056-5 | CAS number: 874195-61-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 Oct - 12 Dec 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 2010 and considered the Question-and-Answer Document by the German Federal Environment Agency (Version 2012-03-02).
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 2008
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium für Arbeit, Gesundheit und Soziales des Landes Nordrhein-Westfalen
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
A direct weighing was prepared to give the test item concentrations. The test item was added into Erlenmeyer flasks (incubation vessels) to about 130 mL deionised water and was stirred before testing (equilibration phase) overnight for 17 hours. The pH was measured was adjusted with NaOH. - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Origin: Aeration tank of a domestic waste water treatment plant (Municipal WWTP Cologne-Stammheim)
- Date of collection: 2014-10-27
- Pretreatment: The sludge was settled and the supernatant was decanted. After centrifuging the sludge (15 min at 4500 rpm and 20°C) the supernatant was decanted again.
- Method of cultivation: Approximately 1 g of the wet sludge was dried in order to calculate the amount of wet sludge to achieve a concentration of activated sludge of 3 g/L (dry weight) suspended solids. The sludge was aerated at 20 ± 2 °C and daily fed with synthetic medium*
- Initial biomass concentration: Before use the wet weight/dry weight relationship of the activated sludge was determined by drying 10 mL of sludge suspension. Subsequently, a sludge suspension of 2 g (dry weight)/L was prepared. 8 mL of the synthetic medium and 100 mL of activated sludge were added to the dissolved test item. The mixture was filled up with deionised water to 250 mL and aerated at 20 ± 2 °C. The inoculum concentration in the test was equal to 800 mg/L suspended solids.
* Synthetic medium:
16.0 g/L peptone
11.0 g/L meat extract
3.0 g/L urea
0.7 g/L NaCl
0.4 g/L CaCl2 x 2H2O
0.2 g/L MgSO4 x 7H2O
2.8 g/L K2HPO4 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.2-8.0
- Nominal and measured concentrations:
- Test item concentration (nominal): 100 mg/L
Reference substance concentrations (nominal): 2.5, 5, 10, 20 and 40 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL glass Erlenmeyer flasks
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- Additional control to determine the physico-chemical oxygen consumption: 1
- Additional control of heterotrophic oxidation (replicates): 2 (100 mg/L test item, containing 1.25 mL of ATU-solution (N-allylthiourea), which equals to a final concentration of 11.6 mg ATU/L)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water and synthetic medium
OTHER TEST CONDITIONS
- Adjustment of pH: Initial to pH 6-8 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- As no significant inhibitory effect was measured at a limit test item concentration of 100 mg/L no statistical analysis was required to determine the EC50.
- Results with reference substance (positive control):
- EC50 (3h) for 3,5-Dichlorophenol = 8.36 mg/L
The EC50 of the reference compound 3,5-Dichlorophenol should be in the range of 2 – 25 mg/L for total respiration; thus the test is validity criterion is fulfilled
Reference
The respiration rate is classified into two processes of oxidation. The oxidation of organic carbon and the ammonium oxidation (nitrification). The use of the specific nitrification inhibitor, ATU (N-allylthiourea), enables the direct assessment of the inhibitory effects of test substances on heterotrophic oxidation, and by subtracting the oxygen uptake rate in the presence of ATU from the total uptake rate, the effects on the rate of nitrification may be calculated.
The oxygen uptake rates of two additional controls and one replicate of the test item concentration 100 mg/L all in the presence of N-allylthiourea were measured after the exposure period. These values represent the heterotrophic respiration.
Description of key information
Activated sludge respiration inhibition (OECD 209) NOEC >= 100 mg/L (nominal)
Key value for chemical safety assessment
Additional information
One study is available on the toxicity of triafamone (CAS No. 874195-61-6) to activated domestic sludge (Neuhahn, 2014). The study was performed according to OECD guideline 209 and GLP. The test was conducted as limit test with a test item concentration of 100 mg/L. After 3 hours incubation, no statistically significant difference in respiration was determined between the 3 replicates with 100 mg/L triafamone and the 6 control vessels. Thus the NOEC (3h) for respiration inhibition of activated sludge is >= 100 mg/L triafamone.
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