O-isopropyl ethylthiocarbamate

Toxicity to terrestrial arthropods

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to terrestrial arthropods: long-term

Data waiving:

other justification

Justification for data waiving:

other:

Reference 2

Endpoint:

toxicity to terrestrial arthropods: short-term

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Justification for type of information:

.Propan-2-ol (Isopropyl alcohol) is both reagents used in the manufacture of IPETC/ O-isopropyl ethylthiocarbamate . Therefore, Propan-2-ol (Isopropyl alcohol) need to be considered in the assessment of IPETC/O-isopropyl ethylthiocarbamate .

Qualifier:

no guideline followed

Principles of method if other than guideline:

Larvae were grown at low larval density on an axenic, killed yeast medium. After emergence, adults were kept on nutrient medium for three days. After three days feeding, each group of flies is transferred into an air-tight plastic vial containing 2 mL of a 3% sucrose solution supplemented with the tested concentration of alcohol. Toxicity data are expressed as the lethal concentration which kills 50% of the flies after 1, 2 or 3 days of treatment.

GLP compliance:

no

Application method:

other: % sucrose solution supplemented with the tested concentration in an airtight plastic vial

Analytical monitoring:

not specified

Vehicle:

no

Test organisms (species):

Drosophila melanogaster

Animal group:

Diptera (fruit fly (small))

Details on test organisms:

Experiments were carried out on two strains of French origin: a melanogaster strain from Colmar and a simulans strain from Marseille.

Study type:

laboratory study

Limit test:

no

Total exposure duration:

3 d

Test temperature:

25ºC

pH (if soil or dung study):

not applicable

Humidity:

not reported

Photoperiod and lighting:

not reported

Details on test conditions:

Larvae were grown at low larval density on an axenic, killed yeast medium. after emergence, adults were separated in groups of 10 females and 10 males and kept on nutrient medium for three days. After three days feeding, each group of flies is transferred into an air-tight plastic vial containing 2 mL of a 3% sucrose solution supplemented with the tested concentration of alcohol. For each test, 7 concentrations (in a geometric progression of ratio 1.25) are used and, for each concentration, 40 females and 40 males. Toxicity data are expressed as the lethal concentration which kills 50% of the flies (LC50) after 1, 2 or 3 days of treatment.

Nominal and measured concentrations:

not reported

Reference substance (positive control):

not specified

Duration:

1 d

Dose descriptor:

LC50

Effect conc.:

3.2 other: % in diet

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: males

Duration:

1 d

Dose descriptor:

LC50

Effect conc.:

25.1 other: g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: males

Duration:

1 d

Dose descriptor:

LC50

Effect conc.:

3.4 other: % in diet

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: female

Duration:

1 d

Dose descriptor:

LC50

Effect conc.:

26.7 other: g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: female

Duration:

2 d

Dose descriptor:

LC50

Effect conc.:

1.5 other: % in diet

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: males

Duration:

2 d

Dose descriptor:

LC50

Effect conc.:

11.8 other: g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: males

Duration:

2 d

Dose descriptor:

LC50

Effect conc.:

1.7 other: % in diet

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: females

Duration:

2 d

Dose descriptor:

LC50

Effect conc.:

13.4 other: g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: females

Details on results:

Assuming the % in diet is a volume/volume percentrage, and with a specific gravity of 0.7855 g/cm3, the LC50 correspond to:
The 1-day LC50 in males is 25.1 g/L in diet
The 1-day LC50 in females is 26.7 g/L in diet
The 2-day LC50 in males is 11.8 g/L in diet
The 2-day LC50 in females is 13.4 g/L in diet

Validity criteria fulfilled:

not applicable

Remarks:

non-guideline study

Conclusions:

Propan-2-ol (Isopropyl alcohol) is both reagents used in the manufacture of IPETC/O-isopropyl ethylthiocarbamate . Therefore, Propan-2-ol (Isopropyl alcohol) need to be considered in the assessment of IPETC/O-isopropyl ethylthiocarbamate.
Assuming the % in diet is a volume/volume percentrage, and with a specific gravity of 0.7855 g/cm3, the LC50 correspond to:
The 1-day LC50 in males is 25.1 g/L in diet
The 1-day LC50 in females is 26.7 g/L in diet
The 2-day LC50 in males is 11.8 g/L in diet
The 2-day LC50 in females is 13.4 g/L in diet

Description of key information

Propan-2-ol (Isopropyl alcohol) is both reagents used in the manufacture of IPETC/O-isopropyl ethylthiocarbamate. Therefore, Propan-2 -ol (Isopropyl alcohol) need to be considered in the assessment of IPETC/O-isopropyl ethylthiocarbamate.
Assuming the % in diet is a volume/volume percentrage, and with a specific gravity of 0.7855 g/cm3, the LC50 correspond to:
The 1-day LC50 in males is 25.1 g/L in diet
The 1-day LC50 in females is 26.7 g/L in diet
The 2-day LC50 in males is 11.8 g/L in diet
The 2-day LC50 in females is 13.4 g/L in diet

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil dwelling arthropods:

11 800 mg/kg soil dw

Long-term EC10, LC10 or NOEC for soil dwelling arthropods:

2 360 mg/kg soil dw

Additional information

Propan-2-ol (Isopropyl alcohol) is both reagents used in the manufacture of Reaction mass of O-isopropyl ethylthiocarbamate and n-butanol and propan-2-ol. Therefore, Propan-2-ol (Isopropyl alcohol) need to be considered in the assessment of Reaction mass of O-isopropyl ethylthiocarbamate and n-butanol  and propan-2-ol .

Assuming the % in diet is a volume/volume percentrage, and with a specific gravity of 0.7855 g/cm3, the LC50 correspond to:

The 1-day LC50 in males is 25.1 g/L in diet

The 1-day LC50 in females is 26.7 g/L in diet

The 2-day LC50 in males is 11.8 g/L in diet

The 2-day LC50 in females is 13.4 g/L in diet