O-isopropyl ethylthiocarbamate

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

(Q)SAR

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Justification for type of information:

QSAR prediction:US EPA accepted QSAR method for chemicals properties assessment.

Qualifier:

according to guideline

Guideline:

other: QSAR

Principles of method if other than guideline:

ECOSAR Program (v1.00)

GLP compliance:

no

Remarks:

not applicable to QSAR models

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

other: earthworm

Animal group:

other: QSAR model

Study type:

other: QSAR

Substrate type:

other: QSAR

Limit test:

no

Total exposure duration:

14 d

Test temperature:

not applicable. QSAR model

pH:

not applicable. QSAR model

Moisture:

not applicable. QSAR model

Details on test conditions:

SMILES : O(C(=NCC)S)C(C)C
CHEM : Carbamothioic acid, ethyl-, O-(1-methylethyl) ester
CAS Number: 141-98-0
MOL FOR: C6 H13 N1 O1 S1
MOL WT : 147.24
Log Kow: 3.32 (KowWin estimate)
Wat Sol: 114.4 mg/L (WskowWin estimate)

Reference substance (positive control):

no

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

186.87 mg/kg soil ww

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

not specified

Remarks on result:

other: 1 kg of water has a volume of 1 L and the mg/kg and mg/L are the same.

Details on results:

LC50 (14-day, earthworm)= 186.870 mg/L (ppm)
1 kg of water has a volume of 1 L and the mg/kg and mg/L are the same.
LC50 (14-day, earthworm)=186.870 mg/kg soil dw

Reported statistics and error estimates:

US EPA accepted QSAR method for chemicals properties assessment.

Overview of QSARs estimations on toxicity of O-isopropyl ethylthiocarbamate (IPETC ) to soil macroorganisms

Method	Results	Remarks	Reference
QSAR estimate	LC50 (14-day, earthworm)= 186.870 mg/L (ppm)	Reliable withrestrictions, QSAR estimated data	ECOSAR Program (v1.00)

 

 

LC50 (14-day, earthworm)= 186.870 mg/kg soil dw

Validity criteria fulfilled:

yes

Remarks:

US EPA accepted QSAR method for chemicals properties assessment.

Conclusions:

LC50 (14-day, earthworm)= 186.870 mg/L (ppm)
1 kg of water has a volume of 1 L and the mg/kg and mg/L are the same.
LC50 (14-day, earthworm)=186.870 mg/kg soil dw

Executive summary:

The ECOSAR QSAR model predicts that 14-day, LC50 (14-day, earthworm) would to be 186.870 mg/L (ppm).

1 kg of water has a volume of 1 L and the mg/kg and mg/L are the same.

LC50 (14-day, earthworm)= 186.870 mg/kg soil dw

Reference 2

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Propan-2-ol (Isopropyl alcohol) is both reagents used in the manufacture of IPETC/ O-isopropyl ethylthiocarbamate . Therefore, Propan-2-ol (Isopropyl alcohol) need to be considered in the assessment of IPETC/O-isopropyl ethylthiocarbamate .

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

not specified

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Caenorhabditis elegans

Animal group:

nematods

Details on test organisms:

SPECIES #
(NAME): 4550 Caenorhabditis elegans
(Nematode)
AGE: NR NR
LIFE STAGE: larva(e)
COMMENT: NR

Study type:

laboratory study

Substrate type:

not specified

Limit test:

no

Total exposure duration:

24 h

Test temperature:

not reported

pH:

not reported

Moisture:

not reported

Details on test conditions:

6.55 % v/v percent volume per volume

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

6.55 other: % v/v

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: % v/v - percent volume per volume

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

6.7 other: % v/v

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: % v/v - percent volume per volume

Details on results:

A 24 h LC50 of 6.5 to 6.6 % v/v - percent volume per volume has been determined for mortality to Caenorhabditis elegans of the test substance.

The toxicities of 4 short-chain alcohols--namely methanol, ethanol, iso-propanol and iso-butanol--were compared in the nematode Caenorhabditis elegans using several different ecotoxicological endpoints. Range-finding tests were conducted using transgenic PC161 worms carrying a double reporter construct (GFP plus lacZ) linked to the stress-inducible hsp16-1 promoter. These tests showed little response from the GFP reporter, but gave good dose-response curves for the lacZ reporter--showing clear induction at 0.5% v/v ethanol in an overnight assay, but only at 4% in a shorter 6-h assay.

Comparison of the short-term dose-response curves shows a confusing pattern of differences between the four alcohols tested, although dose-dependence is evident across at least part of the concentration range. Feeding inhibition assays are somewhat inconclusive with regard to alcohol type, although iso-butanol and iso-propanol appear more toxic than ethanol, while methanol is least toxic. To resolve some of the remaining ambiguities, we also used a fecundity assay to show that iso-propanol is more toxic than ethanol, and a lethality assay to show that iso-butanol is more toxic than iso-propanol.

Most of the endpoints studied are consistent with the following order of toxicity: iso-butanol > iso-propanol > ethanol > or = methanol.

Validity criteria fulfilled:

not specified

Conclusions:

A 24 h LC50 of 6.5 to 6.7 % v/v - percent volume per volume has been determined for mortality to Caenorhabditis elegans of the test substance.
Propan-2-ol (Isopropyl alcohol) is both reagents used in the manufacture of Reaction mass of O-isopropyl ethylthiocarbamate and n-butanol and propan-2-ol. Therefore, Propan-2-ol (Isopropyl alcohol) need to be considered in the assessment of IPETC/O-isopropyl ethylthiocarbamate .

Description of key information

The ECOSAR QSAR model predicts that 14-day, LC50 (14-day, earthworm) would to be 186.870 mg/L (ppm).
1 kg of water has a volume of 1 L and the mg/kg and mg/L are the same.
LC50 (14-day, earthworm)= 186.870 mg/kg soil dw

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

186.87 mg/kg soil dw

Long-term EC10, LC10 or NOEC for soil macroorganisms:

37.374 mg/kg soil dw

Additional information

The ECOSAR QSAR model predicts that 14-day, LC50 (14-day, earthworm) would to be 186.870 mg/L (ppm).

1 kg of water has a volume of 1 L and the mg/kg and mg/L are the same.

LC50 (14-day, earthworm)= 186.870 mg/kg soil dw.