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EC number: 425-450-9 | CAS number: 294-90-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: 92/69/EWG, C.2
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Analysis of the test medium, which were conducted during the experiment, confirming the conformity of the actual concentrations with the nominal concentrations.
- Details on test solutions:
- Identity and concentration of auxiliary solvent for dispersal: M4, Vorschrift Dr. Elendt-Schneider
- Test organisms (species):
- Daphnia magna
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg CaCO3/L
- pH:
- The pH of the test medium rose with the increasing concentration of test substance (Alkaline effect).Another test was conducted with HCl to keep HCl at 8.1
- Nominal and measured concentrations:
- Nominal concentrations of 10 to 100 mg / l.
- Details on test conditions:
- Test was conducted in duplicates with 10 Dapnias per vessel
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 86 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 34 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 100 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Details on results:
- %Concentration loss over test: 0 ... 5In the test series with adjusted pH (8.1) a slightly increased toxicity with respect to the immobilization of Daphnia Magna was observed with 10 mg/ L NOEC. In the test series with increasing pH, the EC50 value of 100 mg / l changed after 24 hours to 45 mg / L. After 48 hours, with a confidence interval of 95%. The EC50 values were determined with 39-57 mg / l.In the test series with a constant pH (8.1), the EC50 value of 86 mg / l changed after 24 hours to 34 mg / l after 48 hours, with a confidence interval of 95%, the EC50 values of 26-54 mg / l were determined ,
- Conclusions:
- The caustic effect of the test substance had no influence on the toxicity. EC50(24h): 86mg/LEC50(48h): 34 mg/LEC100(48h): 100mg/LNOEC(48h): 10mg/L
- Executive summary:
The short term toxicity (48h) of test substance was performed in Daphnia Magna according to the 92/69/EWG, C.2 Guideline under GLP conditions.
The test was conducted twice unbuffered and buffered with HCl. The caustic effect of the test substance had no influence on the toxicity. The test substance short-term (24 and 48 h) effect parameters (mobility based) to Daphnia Magna were: EC50(24h): 86mg/L; EC50(48h): 34 mg/L; EC100(48h): 100mg/L; NOEC(48h): 10mg/L.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Test organisms (species):
- Daphnia magna
- Total exposure duration:
- 48 h
- Hardness:
- 254.22 mg CaCO3/ L
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 73.3 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 25.1 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 50 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 mg/L
- Details on results:
- %Concentration loss over test: 1 ... 7
- Conclusions:
- The test substance short-term (48 h) toxicity parameters in Daphnia Magna were:EC50(24h): 73.3 mg/LEC50(48h): 25.1 mg/LEC100(48h):50 mg/LNOEC(48h): 12.5 mg/L
- Executive summary:
The short term toxicity (48h) of test substance was performed in Daphnia Magna according to the OECD 202 Guideline under GLP conditions.
The test substance short-term (24 and 48 h) effect parameters (mobility based) in Daphnia Magna were: EC50(24h): 73.3 mg/L; EC50(48h): 25.1 mg/L; EC100(48h):50 mg/L; NOEC(48h): 12.5 mg/L.
Referenceopen allclose all
Toxic in the concentrations > 12.5 mg/L;
Analytical measurements by DOC analysis
Description of key information
Short-term toxicity to aquatic invertebrates. Key study (001).Test method according to OECD 202 Guideline. GLP study.The short term toxicity (48h) of test substance was performed in Daphnia Magna.
EC50(48h): 25.1 mg/L.
Short-term toxicity to aquatic invertebrates. Key study (002). Test method according to 92/69/EWG, C.2 Guideline, comparable to guideline with acceptable restrictions. GLP study.The short term toxicity (48h) of test substance was performed in Daphnia Magna.The test was conducted twice unbuffered and buffered with HCl. The caustic effect of the test substance had no influence on the toxicity.
EC50(48h): 34 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 25.1 mg/L
Additional information
Justification for selection of short-term toxicity to aquatic invertebrates endpoint
Study 001 was selected because it is following the most recent guideline (OECD 202).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.