Benzoic acid, 3,5-diamino-4-[2-[4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-2-[2-[2-sulfo-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-, sodium salt (1:?)

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Qualifier:

according to guideline

Guideline:

ISO DIS 9439 (Ultimate Aerobic Biodegradability - Method by Analysis of Released Carbon Dioxide)

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, adapted

Duration of test (contact time):

29 d

Initial conc.:

51 mg/L

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance:

acetic acid, sodium salt

Parameter:

% degradation (CO2 evolution)

Value:

13

Sampling time:

29 d

Details on results:

Points of degradation plot (test substance, average of 2 tests):
1 % degradation after 2 d
2 % degradation after 5 d
3 % degradation after 7 d
3 % degradation after 9 d
5 % degradation after 14 d
6 % degradation after 19 d
10 % degradation after 23 d
12 % degradation after 27 d
13 % degradation after 29 d

Results with reference substance:

Points of degradation plot (reference substance):
3 % degradation after 2 d
37 % degradation after 5 d
57 % degradation after 7 d
66 % degradation after 9 d
71 % degradation after 14 d

The test substance was not readily biodegradable under the conditions of the modified Sturm test presently performed.

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable, not fulfilling specific criteria

Executive summary:

Determination of ready biodegradability: CO2 evolution test with Everzol Orange ED-G Crude. The concentration of Everzol Orange ED-G was 51 mg/l. The test substance was added to duplicate test bottles and the toxicity control.

The relative degradation values calculated from the measurements performed during the test revealed 19 and 8% degradation of Everzol Orange ED-G Crude, for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was no met. In the toxicity control, Everzol Orange ED-G crude was found not to inhibit microbial activity.

In conclusion, Everzol Orange ED-G Crude is not readily biodegradable.

Description of key information

The test substance was not readily biodegradable under the test conditions.

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable, not fulfilling specific criteria

Additional information

Determination of ready biodegradability: CO2 evolution test with Everzol Orange ED-G Crude. The concentration of Everzol Orange ED-G was 51 mg/l. The test substance was added to duplicate test bottles and the toxicity control.

The relative degradation values calculated from the measurements performed during the test revealed 19 and 8% degradation of Everzol Orange ED-G Crude, for the duplicate bottles tested. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was no met. In the toxicity control, Everzol Orange ED-G Crude was found not to inhibit microbial activity.

In conclusion, Everzol Orange ED-G Crude is not readily biodegradable.