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Diss Factsheets
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EC number: 227-774-4 | CAS number: 5977-14-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Study conducted prior to GLP and OECD guideline implementation.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- not specified
- GLP compliance:
- no
- Remarks:
- Study conducted prior to GLP and OECD guideline implementation.
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Acetoacetamide
- EC Number:
- 227-774-4
- EC Name:
- Acetoacetamide
- Cas Number:
- 5977-14-0
- Molecular formula:
- C4H7NO2
- IUPAC Name:
- acetoacetamide
- Test material form:
- liquid
- Details on test material:
- Acetoacetamide solution 25 %
white crystaline
Constituent 1
- Specific details on test material used for the study:
- 25% Acetoacetamide solution
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- - Source: Hoechst breeding colony;
- Strain: WISKf (SPF 71)
- Weight at study initiation: 99 - 115 grams
- Fasting period before study: 16 hours
- Housing: plastic cages with wood shavings
- Diet: Altromin 1324, ad libitum
- Water: Tap-water, ad libitum
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- 25% solution of acetoacetamide in water
- Doses:
- 15000 mg/kg bw
- No. of animals per sex per dose:
- 10
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observation: daily; weighing: once a week
- Necropsy of survivors performed: yes - Statistics:
- No statistics was performed since only one concentration was applied.
Results and discussion
- Preliminary study:
- In a pre-test males didn't show a higher sensitivity.
Effect levelsopen allclose all
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 15 000 mg/kg bw
- Based on:
- test mat. (dissolved fraction)
- Remarks:
- 25% solution
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- ca. 3 750 mg/kg bw
- Based on:
- test mat.
- Remarks:
- calculated value
- Mortality:
- no
- Clinical signs:
- other: tumbling gait, prone position
- Gross pathology:
- no abnormal findings
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The LD50 was determined to be >15000 mg/kg bw.
Calculated for the pure substance: LD50 = 3750 mg/kg bw. - Executive summary:
The acute oral toxicity of the test item was determined by application of 15000 mg/kg bw as a 25% solution in water to 10 female Wistar rats.
After application animals showed tumbling gait and prone position. During the observation period of 14 days body weight gain was not affected and no deaths occured.
Necropsy revealed no abnormal findings. The LD50 was determined to be >15000 mg/kg bw.
Calculated for the pure substance: LD50 = 3750 mg/kg bw.
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