Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 227-774-4 | CAS number: 5977-14-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 September 2017 - 29 November 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- The following nominal test item concentrations were tested (spaced by a factor of 3.2): 100, 31.3, 9.77, 3.05, 0.954 mg/L and control.
The necessary amount of test item for preparing the stock solution S1 was weighed on a weighing scoop and transferred to a volumetric flask.
Test medium was added up to the bench mark and the solution was homogenised by shaking. Afterwards the solution was clear and
transparent. Lower test solutions were prepared by dilution of appropriate solutions with test medium. All stock and test solutions were prepared
with AAP-medium containing algae cells with a target density of 0.5 × 104 cells per mL. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- strain: SAG 61.81
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 22.7 – 23.0 °C
- pH:
- 7.27 to 8.48
- Nominal and measured concentrations:
- The measured initial concentrations of acetoacetamide ranged from 78% to 85 % of nominal. In the aged samples the measured content was between 90 % and 91 % of nominal.
- Details on test conditions:
- The mean light intensity was 88.8 μEm-2s-1 at cell culture level.
Six replicates were employed for the control and three for each test item concentration.
The test was performed in 100 mL Erlenmeyer flasks each containing ~ 50 mL test solution. The pH was recorded at test start and test
end. Temperature was measured continuously over the whole test period and recorded daily. Light intensity of the continuous illumination was
measured at test start. - Reference substance (positive control):
- yes
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 83.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 31.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 27.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Reported statistics and error estimates:
- Where possible NOEC and LOEC were determined by using a multiple comparison method.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Due to an inhibition of all parameters (growth rate, yield) below 50 % the database was weak for probit analysis, hence the EC10-value for yield was
not determined and the EC10-, EC20- and EC50-value for growth rate and the EC20- and EC50-value for yield were considered to be > 100 mg/L (nominal)
and > 83.8 mg/L (actual). - Executive summary:
A toxicity study to single cell green alga Desmodesmus subspicatus was performed in year 2017 according to OECD Guideline 201 and GLP.
Significant inhibitory effects were determined for growth rate and yield at test item concentrations of 100 mg/L (nominal) and 83.8 mg/L (actual).
The overall LOEC was therefore determined to be 100 mg/L (nominal) and 83.8 mg/L (actual), the corresponding NOEC was set at 31.3 mg/L (nominal) and 27.5 mg/L (actual).
Due to an inhibition of all parameters (growth rate, yield) below 50 % the database was weak for probit analysis, hence the EC10-value for yield was
not determined and the EC10-, EC20- and EC50-value for growth rate and the EC20- and EC50-value for yield were considered to be > 100 mg/L (nominal)
and > 83.8 mg/L (actual).
Reference
Description of key information
A toxicity study to single cell green alga Desmodesmus subspicatus was performed in year 2017 according to OECD Guideline 201 and GLP.
Significant inhibitory effects were determined for growth rate and yield at test item concentrations of 100 mg/L (nominal) and 83.8 mg/L (actual).
The overall LOEC was therefore determined to be 100 mg/L (nominal) and 83.8 mg/L (actual), the corresponding NOEC was set at 31.3 mg/L (nominal) and 27.5 mg/L (actual).
Due to an inhibition of all parameters (growth rate, yield) below 50 % the database was weak for probit analysis, hence the EC10-value for yield was
not determined and the EC10-, EC20- and EC50-value for growth rate and the EC20- and EC50-value for yield were considered to be > 100 mg/L (nominal)
and > 83.8 mg/L (actual).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 83.8 mg/L
- EC10 or NOEC for freshwater algae:
- 27.5 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.