L-Alanine, N-coco acyl derivs., sodium salts

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March 16, 2005 - April 13, 2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

Version / remarks:

1992

GLP compliance:

yes (incl. QA statement)

Remarks:

Fraunhofer-Institute for Molecular Biology and Applied Ecology (IME), 57377 Schmallenberg (Germany)

Test material

Test material information

Constituent 1

Reference substance name:

L-Alanine, N-coco acyl derivs., sodium salts

EC Number:

290-478-9

EC Name:

L-Alanine, N-coco acyl derivs., sodium salts

Cas Number:

90170-45-9

Molecular formula:

RHNNaO3

IUPAC Name:

L-Alanine, N-coco acyl derivs., sodium salts

Test material form:

solid

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Ajinomoto Co., Inc., AminoScience Lab. ALG-2, 1-1 Suzuki-Cho, Kawasaki-ku, Kawasaki-Shi, Kanagawa, Japan, 210-8681//Batch/Lot number: 050126BP
- Expiration date of the lot/batch: 01.02.2007
- Purity test date: 17.01.2005

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Fresh samples of secondary effluent were withdrawn on March 16th, 2005 from the sewage treatment plant in 57392 Schmallenberg (Germany), which was mainly fed with municipal wastewater
- Laboratory culture: no
- Preparation of inoculum for exposure: On return to the laboratory the secondary effluent was allowed to settle for 1 h and was then centrifuged. The decanted effluent was kept aerobic until required.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

30 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: according to OECD 301E
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 22 +/- 2°C
- pH: not reported
- pH adjusted: no
- Continuous darkness: yes (diffused light)


TEST SYSTEM
- Culturing apparatus: conical glass flasks
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: The secondary effluent was kept aerobic until use in the test
- Measuring equipment: TOC analyser
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: yes

SAMPLING
- Sampling frequency: The concentrations of DOC in samples from each flask were determined in duplicate at test start and at day 1, 3, 5, 7, 8, 14, 21, and 28 in a TOC analyser.

CONTROL AND BLANK SYSTEM
- Inoculum blank: performed
- Abiotic sterile control: performed
- Toxicity control: performed
- Other: procedural control with reference item sodium benzoate performed

Reference substance

Reference substance:

benzoic acid, sodium salt

Remarks:

30 mg DOC/L

Results and discussion

% Degradation

Key result

Parameter:

% degradation (DOC removal)

Value:

94.4

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test item proved to be readily biodegradable (91.4% biodegradation after 14 d, 94.4% biodegradation after 28 d).

Executive summary:

The ready biodegradation of L-Alanine, N-coco acyl derivs., sodium salts was investigated in a study conducted according to OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test; 1992) over a period of 28 days and using secondary effluent as inoculum collected from a sewage treatment plant which is mainly fed with municipal wastewater. The biodegradation rate was determined by measurement of DOC removal. Inoculum blank, adsorption control, procedural/functional control with the reference substance sodium benzoate, and toxicity control using 30 mg DOC/L test item and 30 mg DOC/L reference compound were performed.

 

This study is regarded as reliable without restriction and satisfies the guideline requirements for ready biodegradation.

 

The test item proved to be readily biodegradable and fulfilling the 10-d-window criterion (91.4% biodegradation after 14 d; 94.4% biodegradation after 28 d of incubation). The functional control reached the pass level > 60% after 14 d. In the toxicity control containing both test and reference item 93.7% biodegradation based on DOC removal occurred within 14 d thus indicating that the test item was not inhibitory at the concentration tested.