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EC number: 257-297-7 | CAS number: 51581-32-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
In vivo GLP guideline studies are available for effect to the skin and the eyes, according to OECD 404 and OECD 405, respectively.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-02-23 to 1999-07-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Version / remarks:
- adopted on 17 July, 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - CAS No. : 51581-32-9
- Batch No.: TMFP 304
- Purity: At least 99 %
- Solubility in water: soluble
- Storage: in the refrigerator, in the dark
- Date of expiry: December 1999
- Appearance: Colorless liquid
pH: 7.29 - Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: J. Schitkovits, A-2620 Natschbach
- Weight (at study initiation and termination) : Animal no. 1: 2.9 kg and 3.1 kg, Animal no. 2: 2.7 kg and 2.9 kg, Animal 3: 2.8 kg and 2.8 kg
- Housing: Individual caging in metal wire cages
- Diet (e.g. ad libitum): Yes
- Water (e.g. ad libitum): Yes
- Acclimation period: 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20
- Humidity (%): 53
- Air changes (per hr): 12
- Photoperiod (hrs dark / hrs light): 12 / 12 - Type of coverage:
- semiocclusive
- Preparation of test site:
- clipped
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not specified
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 mL - Duration of treatment / exposure:
- 4 h
- Observation period:
- 1, 24, 48 and 72 h after patch removal
- Number of animals:
- 3
- Details on study design:
- TEST SITE
- Area of exposure: 2.5 cm X 2.5 cm
OBSERVATION TIME POINTS : 1, 24, 48 and 72 h
(indicate if minutes, hours or days)
SCORING SYSTEM:
- Method of calculation: Scoring terms of 24, 48 and 72 h after the patch removal were used - Irritation parameter:
- erythema score
- Basis:
- animal: 1, 2 and 3
- Time point:
- 24/48/72 h
- Score:
- 0
- Irritation parameter:
- edema score
- Basis:
- animal: 1, 2 and 3
- Time point:
- 24/48/72 h
- Score:
- 0
- Irritant / corrosive response data:
- All areas treated with the test substance and all control areas were normal before the application and at each observation time
- Other effects:
- - Other adverse local effects:
None
- Other adverse systemic effects: None - Interpretation of results:
- GHS criteria not met
- Conclusions:
- In this study, the test substance is considered non-corrosive or a non-irritant to the skin.
- Executive summary:
In an in vivo skin irritation study conducted according to the OECD guideline 404, 0.5 mL of the test item was spread on cellulose patches in a size of about 2.5 cm X 2.5 cm and were applied to test sites on each of 3 New Zealand White rabbits for 4 hours. The patches were then removed and the skin was examined for erythema / eschar and oedema as well as other local signs approximately 1, 24, 48 and 72 hours after patch removal. No general toxic effects were observed. All areas treated with test substance and all control areas were normal before the application and at each observation time. Based on these results, the test substance is considered as a non-irritant to the skin under the UN GHS Criteria.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-02-23 to 1999-07-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- Adopted, 12 February 1999
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - CAS No.: 51581-32-9
- Batch No.: TMFP 304
- Purity: At least 99 %
- Solubility in water: Soluble
- Storage: In the refrigerator, in the dark
- Date of expiry: December 1999
- Appearance: Colorless liquid
- pH of the test substance: 7.29 (10 % solution in deionised water, v/v, determined with a pH-meter) - Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- - Supplier: J. Schitkovits, A-2620 Natschbach
- Justification for selection of the species: Recommended by the Directive / Guideline
- Number and sex: 3 females
- Body weight (at the start and at the termination of the test): Animal No. 11: 3.0 kg and 3.5 kg, Animal No. 12: 3.2 kg and 3.6 kg, Animal No. 13: 3.2 kg and 3.k - Housing: Individual caging in metal wire cages
- Diet (e.g. ad libitum): Yes
- Water (e.g. ad libitum): Yes
- Acclimation period: 10 days (animal no. 11) and 16 days (animal no. 12 and no. 13)
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21
- Humidity (%): 53
- Air changes (per hr): 12
- Photoperiod (hrs dark / hrs light): 12 / 12 - Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL - Duration of treatment / exposure:
- The test substance was administered to the conjunctival sac of the right eye by gently pulling the lower lid away from the eyeball for instillation. The eye was held closed for about one second to prevent loss of test substance.
- Observation period (in vivo):
- Both eyes of the animals were examined within 24 hours before instillation and approximately 1, 24, 48 and 72 hours p.a.
Additional examinations were performed 6, 8, 10, 13, 15, 17 and 21 days after the instillation, ending individually for each animal. - Number of animals or in vitro replicates:
- 3
- Details on study design:
- SCORING SYSTEM:
The scoring terms of 24, 48 and 72 h after the instillation were used
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0.7
- Max. score:
- 4
- Reversibility:
- fully reversible within: 13d
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 21d
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 6d
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 2
- Reversibility:
- fully reversible within: 10d
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 2
- Reversibility:
- fully reversible within: 6d
- Irritation parameter:
- conjunctivae score
- Remarks:
- redness
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 13d
- Irritation parameter:
- conjunctivae score
- Remarks:
- redness
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 2.7
- Max. score:
- 3
- Reversibility:
- not fully reversible within: 21d
- Irritation parameter:
- conjunctivae score
- Remarks:
- redness
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 3
- Reversibility:
- fully reversible within: 8d
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- fully reversible within: 8d
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 3.7
- Max. score:
- 4
- Reversibility:
- fully reversible within: 17d
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 6d
- Irritant / corrosive response data:
- Ocular lesions were reversible in 2 / 3 animals within 17 days p.a. and were not reversible in 1 / 3 animals within 21 days
- Other effects:
- Other observations:
- Cornea: Diffuse areas of opacity (score "1") raised to easily discernible translucent areas with details of iris slightly obscured (score "2"). Most of the time the whole cornea was affected. White coverings on the sclera were noted in one animal 72 h p.a. and corneal lesions were still present 21 d p.a. In 2 / 3 animals, lesions were fully reversible. The corneae became normal again 6 d - 13 d p.a.
- Iris. In 2 / 3 animals mild changes (score "1") were noted from 24 h p.s. on and lasted until a maximum of 8 d p.a.
- Conjunctivae, redness: Injected blood vessels to diffuse beefy red colour were noted in all animals. The lesions were observed from 1 h p.a. to a maximum of 10 d p.a. in 2 / 3 animals and lasted until 21 d p.a. in the third animal. In 1/ 3 animals, a turbid ocular discharge from the instilled eye was seen 48 h p.a.
- Conjunctivae, chemosis: In all animals swelling in different degrees of severity were noted from 1 h onwards. The lesions then gradually returned to normal and 6, 8 and 17 d p.a. no swellings were detectable. - Interpretation of results:
- Category 1 (irreversible effects on the eye) based on GHS criteria
- Conclusions:
- In this study, Pyridobase caused irreversible serious ocular lesions in 1 / 3 animals and was irritating to the eyes in 2 / 3 animals.
- Executive summary:
In an in vivo eye irritation study conducted according to the OECD guideline 405, 0.1 mL of the test item was instilled to the conjunctival sac of one eye of each of 3 New Zealand White rabbits and the other eye was used as a control. The eyes were observed for body weights, general signs of toxicity and eye examinations. Irreversible ocular lesions in 1 / 3 animals and irritation in 2 / 3 animals was observed. Based on these results, the test substance is considered as an irritant under 'Category 1' according to the UN GHS Criteria.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irreversible damage)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Justification for classification or non-classification
Based on irreversible ocular lesions in 1 out of 3 animals, the test substance is considered to cause serious eye damage. Classification as Eye Damage 1 is warranted under CLP.
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