4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23-08-2017 - 28-09-2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

GLP compliance:

no

Specific details on test material used for the study:

Test Item Name 4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride
CAS No. 6358-36-7
Batch No. KCP/FS/44/17
Physical Appearance Yellow powder
Date of manufacturing 2017-05-01
Date of expiry 2017-04-12
Purity not avaiable in COA
Stability Stable in recommended storage conditions
Storage Conditions 1. Ambient Temp (23 to 27 °C) (Keep container tightly closed in a dry & well-ventilated place. Store in cool place. Do not expose to air, light & moisture.)
Precautions Wear respiratory protection. Avoid dust formation. Avoid breathing vapours, mist or gas. Ensure adequate ventilation. Evacuate
personnel to safe areas. Avoid breathing dust.
Molecular Formula C21H30ClN3
Molecular Weight 359.942

Oxygen conditions:

aerobic

Inoculum or test system:

other: Mixed Inoculum

Details on inoculum:

Mixed Inoculum Preparation:1 capsule of polyseed was dissolved into 500ml DI water and stirred it for 1 hour for proper mixing. This gave the
bacterial count as 107 to 108 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of
microorganism.

Duration of test (contact time):

35 d

Initial conc.:

4 mg/L

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

BOD

Details on study design:

1. The test item, i.e., 4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride was taken at a concentration of 0.5ml/125ml The
reference item chosen was taken at a concentration of 0.5ml/125ml in the mineral medium was directly added to the BOD bottle.
2. The BOD bottle of test item& reference item were filled with 120ml &120.5ml of mineral media, respectively.
3. All the BOD bottles were inoculated with prepared mixed inoculum (Polyseed) at the concentration of 32ml/L.. This concentration gave the
bacterial CFU/ml as 107to 108.
4. Analyse all the zero-time bottles immediately for dissolved oxygen by the electrode methods. Stopper the remaining replicate bottles ensuring
that no air bubbles are enclosed, and incubate at 20°C in the dark.
5. Each series must be accompanied by a complete parallel series for the determination of the inoculated blank medium.
6. Withdraw at least duplicate bottles of all series for dissolved oxygen analysis at time intervals (at least weekly) over the 35 days incubation.
7. For N-containing test substances, corrections for uptake of oxygen by any nitrification occurring should be made.

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (O2 consumption)

Remarks:

BOD

Value:

22.51

Sampling time:

35 d

Results with reference substance:

Sodium benzoate: 73.49% after 35 days

TABLE 1

D.O Values(mg/L)

No. of Days	Inoculum Blank (Control)	Test Suspension	Procedure Control (Reference Item)
0	6.6	6.5	6.6
7	6	4.5	3.9
14	5.8	3.2	2.5
21	5.7	3	1.2
28	5.6	2.8	0.7
35	5.4	2.5	0.5

 

TABLE 2

BOD values (mgO2/mg)

No. of Days	Test Suspension	Procedure Control (Reference Item)
0	0	0
7	0.37	0.52
14	0.47	0.82
21	0.5	1.12
28	0.5	1.22
35	0.52	1.22

 

TABLE 3

PERCENT BIODEGRDATION RESULTS

No. of Days	Test Suspension	Procedure Control (Reference Item)
0	0%	0%
7	16.01%	31.32%
14	20.34%	49.39%
21	21.64%	67.46%
28	21.64%	73.49%
35	22.51%	73.49%

 

Table 4

BOD35, THOD AND % BIODEGRADATION VALUES

Method details	BOD35 (mgO2/mg)	ThOD (mgO2/mg)	% Biodegradation
Test Item	0.52	2.31	22.51
Reference Item	1.22	1.66	73.49

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

The test item 4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride(CAS No.6358-36-7) undergoes 22.51 % biodegradation after 35 days in
the test condition. Thus, the test item was considered to be primary inherent biodegradable.

Executive summary:

35-days Closed Bottle test following the OECD guideline 301D to determine the ready biodegradability of the test item 4,4'-carbonimidoylbis[N,Ndiethylaniline]

monohydrochloride, CAS No. 6358-36-7. The test system included control, test item and reference item. The concentration of test and reference item ( Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD35 value of 4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride, CAS No. 6358-36-7 was observed to be 0.52 mgO2/mg. ThOD was calculated as 2.31 mgO2/mg. Accordingly, the % degradation of the test item after 35 days of incubation at 20 ± 1°C according to Closed Bottle test was found to be 22.51%. Based on the results, the test item, under the test conditions, was considered to be primary inherent biodegradable.

Description of key information

The test item 4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride(CAS No.6358-36-7) undergoes 22.51 % biodegradation after 35 days in

the test condition. Thus, the test item was considered to be primary inherent biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable

Additional information

35-days Closed Bottle test following the OECD guideline 301D to determine the ready biodegradability of the test item 4,4'-carbonimidoylbis[N,Ndiethylaniline]

monohydrochloride, CAS No. 6358-36-7. The test system included control, test item and reference item. The concentration of test and reference item ( Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD35 value of 4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride, CAS No. 6358-36-7 was observed to be 0.52 mgO2/mg. ThOD was calculated as 2.31 mgO2/mg. Accordingly, the % degradation of the test item after 35 days of incubation at 20 ± 1°C according to Closed Bottle test was found to be 22.51%. Based on the results, the test item, under the test conditions, was considered to be primary inherent biodegradable.