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EC number: 220-180-6 | CAS number: 2654-57-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard test guidelines
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 48 hrscontinuous stirring for achieving test concentrations of 0.3125mg/L, 0.625mg/L, 1.25mg/L, 2.5mg/L, 5mg/L respectively.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Animal species: Zebra fish(Danio rerio)
Animal breeder/ supplier: Koyal aquarium,Nagpur.
Body length average: 1.73 cm
Body weight: 0.422g (average)
Acclimatization: Fishes, to be used in the study, are kept in the test water for seven days immediately before testing under following conditions:
Photoperiod: 16 hours light and 8 hours dark
Temperature range: 24.5 °C
pH range: 7.65
DO range: 7.7 mg/l
Feeding: Daily once
Aeration: Continuous
Mortality: (%) During last 48 hours = 0 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 150.5 mg of CaCO3
- Test temperature:
- 23 °C
- pH:
- 7.05
- Dissolved oxygen:
- 7.8 mg/l
- Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L
- Details on test conditions:
- Photoperiod: 16 hours light and 8 hours dark
Volume of water: 2 liters
No. of animals per vessel: 8 fishes
Test temperature: 23 °C
Adaptation: Test conditions corresponded to housing conditions
Test dates: Test started at : 29-05-2017
Test Exposure date: 05-06-2017
Date of experimental termination : 09-06-2017
Withdrawal of food: 1 day before and during exposure
Aeration: Aeration in test vessels was provided 1 day before the start of experiment
Test vessels: The test vessels used were all plastic aquaria having the water capacity of 5liters. - Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 0.312 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.625 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.2654-57-1 on Zebra fish(Danio rerio)was found to be 0.625 mg/L.
- Sublethal observations / clinical signs:
Mortality and symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Mortality (cumulated) after
3 h
6 h
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
0.3125
4
4
-
-
-
-
-
-
-
-
-
-
-
-
0.625
4
4
-
-
-
-
-
-
-
-
2
2
2
2
1.25
4
4
-
-
-
-
-
-
4
4
4
4
4
4
2.5
4
4
-
-
4
4
4
4
4
4
4
4
4
4
5
4
4
-
-
4
4
4
4
4
4
4
4
4
4
Visible Symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Symptom (cumulated) after
3 h
6 h
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
0.3125
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
0.625
4
4
M4
M4
M4
M4
M4
M4
M4
M4
M2
M2
M2
M2
1.25
4
4
M4
M4
M4
M4
M2
M2
-
-
-
-
-
-
2.5
4
4
IS4
IS4
-
-
-
-
-
-
-
-
-
-
5
4
4
IS4
IS4
-
-
-
-
-
-
-
-
-
-
Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
pH and oxygen content:
Nominal
Concentration
(mg/l)
Number of
fish
pH readings after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
8.23
8.25
8.31
8.32
8.31
8.32
8.32
8.31
0.3125
4
4
8.13
8.15
8.27
8.29
8.27
8.30
8.30
8.35
0.625
4
4
8.17
8.20
8.30
8.35
8.35
8.36
8.41
8.44
1.25
4
4
8.21
8.25
-
-
-
-
-
-
2.5
4
4
-
-
-
-
-
-
-
-
5
4
4
-
-
-
-
-
-
-
-
Nominal
Concentration
(mg/l)
Number of
fish
Oxygen content (mg/L) after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
6.9
6.9
6.9
6.9
6.9
6.8
6.8
6.8
0.3125
4
4
6.9
6.9
6.8
6.8
6.7
6.7
6.7
6.7
0.625
4
4
6.9
6.9
6.8
6.8
6.7
6.7
6.7
6.7
1.25
4
4
6.9
6.9
-
-
-
-
-
-
2.5
4
4
-
-
-
-
-
-
-
-
5
4
4
-
-
-
-
-
-
-
-
Test temperature:
Nominal
Concentration
(mg/l)
Number of
fish
Temperature (°C) after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
23.2
23.2
23.5
23.5
23.5
23.5
23.6
23.6
0.3125
4
4
23.2
23.2
23.5
23.5
23.5
23.5
23.6
23.6
0.625
4
4
23.2
23.2
23.5
23.5
23.5
23.5
23.6
23.6
1.25
4
4
23.2
23.2
-
-
-
-
-
-
2.5
4
4
-
-
-
-
-
-
-
-
5
4
4
-
-
-
-
-
-
-
-
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 96 h study was conducted for test material on Zebra fish (Danio rerio), at a test concentrations of 0.3125mg/L,0.625mg/L,1.25mg/L,2.5mg/L,5mg/L . The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 was determined to be 0.625 mg/L
- Executive summary:
Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 48 hrscontinuous stirring for achieving test concentrations of 0.3125mg/L, 0.625mg/L, 1.25mg/L, 2.5mg/L, 5mg/L respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be 0.625 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic acute 1 as per the CLP classification criteria.
Reference
Description of key information
Short-term toxicity to fish:
Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 48 hrscontinuous stirring for achieving test concentrations of 0.3125mg/L, 0.625mg/L, 1.25mg/L, 2.5mg/L, 5mg/L respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be 0.625 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic acute 1 as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.625 mg/L
Additional information
Short-term toxicity to fish:
Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with 48 hrscontinuous stirring for achieving test concentrations of 0.3125mg/L, 0.625mg/L, 1.25mg/L, 2.5mg/L, 5mg/L respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be 0.625 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic acute 1 as per the CLP classification criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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