2,3,5-trimethylhydroquinone

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Principles of method if other than guideline:

Internal BASF method was used which was in large part equivalent to OECD guideline 403

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,3,5-trimethylhydroquinone

EC Number:

211-838-3

EC Name:

2,3,5-trimethylhydroquinone

Cas Number:

700-13-0

Molecular formula:

C9H12O2

IUPAC Name:

2,3,5-trimethylbenzene-1,4-diol

Details on test material:

- Name of test material (as cited in study report): 2,3,5-trimethylhydroquinone
- Substance No.: 77/749
- Substance type: yellow powder
- Analytical purity: 99.5%

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: MUS RATTUS, Brunnthal, Germany
- Weight at study initiation: mean 185 g (male), 183 g (female)
- Diet (e.g. ad libitum): Herilan MRH, H . EGGERSMANN KG, Rinteln/Weser, Germany, ad libitum
- Water (e.g. ad libitum): drinking water, ad libitum


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24
- Humidity (%): 50 - 60
- Photoperiod (hrs dark / hrs light): 12 / 12

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

nose/head only

Vehicle:

other: ethanol / Lutrol E 400 (1:1)

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- System of generating particulates/aerosols: Zweistoffdüse (Rhema ), Infu 362 Infusionspumpe (Datex)
- Temperature, humidity, pressure in air chamber: ca. 1.5 Pa


TEST ATMOSPHERE
- Brief description of analytical method used: gas chromatography in DMF; sample was taken with 2 connected Impinger bottles. Amount was up to 30 l with 3 l/min
- Samples taken from breathing zone: yes


VEHICLE
- Composition of vehicle (if applicable): Ethanol p.a. and Lutrol E 400 1:1

Analytical verification of test atmosphere concentrations:

yes

Remarks:

gas chromatography

Duration of exposure:

4 h

Concentrations:

Group 5,4,3,2,1:
analytical concentration: 0.64, 0.87, 1.38, 1.73, 1.96 mg/l
nominal concentration: 1.70, 2.48, 6.26, 9.17, 9.17 mg/l

No. of animals per sex per dose:

20

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: prior to study; days 7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, histopathology

Statistics:

Probit analysis according to D .J . Finney (Probitanalysis 1971, Seite 1 - 150). Published by the Syndics of the Cambridge University Press, Bentley House, 200 Euston Road, London

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC50

Effect level:

1.5 mg/L air

Exp. duration:

4 h

Remarks on result:

other: aerosol

Effect levels 2

Sex:

male

Dose descriptor:

LC50

Effect level:

1.2 mg/L air

Exp. duration:

4 h

Remarks on result:

other: aerosol

Effect levels 3

Sex:

female

Dose descriptor:

LC50

Effect level:

1.7 mg/L air

Exp. duration:

4 h

Remarks on result:

other: aerosol

Mortality:

1.96 mg/l: 9/10 males; 8/10 females
1.73 mg/l: 8/10 males, 6/10 females
1.38 mg/l: 8/10 males, 1/10 females
0.87 mg/l: 1/10 males, 0/10 females
0.64 mg/l: 0/10 males, 0/10 females
Deaths occurred within 2 days after dosing

Clinical signs:

other: 1.38, 1.73, 1.96 mg/l: attempts to escape, eyelid closure, secretion out of eyes and nose, dyspnea, reduced pain reflex, squatting posture, spastic gait, corneal opacity, swollen snout, ruffled fur with substance adhesions. 0.87 mg/l: attempts to escap

Body weight:

Body weight gain was clearly retarded in males of groups 2 and 3 and in females of group 1 at 7 days after exposure. Animals had gained weight at 14 days; however, only body weights of the group-1 females had become similar to control values. Slight retardation of body weight gain was also seen for males of the groups 1 and 4. Body weight gain of males and females of the groups 2, 3, 4, and 5 were not significantly different from control

Gross pathology:

changes of the heart (acute vestibular dilatation, acute congestive hyperemia) and lungs (slight to moderate pulmonary emphysema) in decedents; no pathological findings in survivors.

Any other information on results incl. tables

Body weight:

dose	mean body weight (g)
	prior to study		day 7		day 14
mg/l	male	female	male	female	male	female	Group
1.96	187	185	220	185	258	211	1
1.73	192	182	201	199	250	215	2
1.38	175	185	188	202	246	222	3
0.87	178	178	212	198	256	217	4
0.64	185	183	229	202	279	220	5
0	191	185	235	204	279	215	control