Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
Reaction mass of pentasodium 2-{[4-chloro-6-(ethyl{3-[(2-sulfonatoethyl)sulfonyl]phenyl}amino)-1,3,5-triazin-2-yl]amino}-5-hydroxy-6-({2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl}diazenyl)naphthalene-1,7-disulfonate and tetrasodium 2-[(4-chloro-6-{ethyl[3-(vinylsulfonyl)phenyl]amino}-1,3,5-triazin-2-yl)amino]-5-hydroxy-6-({2-sulfonato-4-[(4-sulfonatophenyl)diazenyl]phenyl}diazenyl)naphthalene-1,7-disulfonate
EC number: 459-580-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 March 2006 to 18 April 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Identity: FAT 40824/A
Batch: Red ROE 805 BOP 04/05
Appearance: dark red powder
Purity: Organic part (Na-salt): approx. 82 %; Main component 1 : approx. 36.2 %; Main component 2: approx. 27.5 %; Oligomers: 10%
Expiration date: 01 October 2010
Stability in water: Max. 7 days at room temperature
Solubility in water: >100g/Lat20°C
Storage: At room temperature at about 20 °C, in a desiccator because test substance is hygroscopic, away from direct sunlight - Analytical monitoring:
- yes
- Details on sampling:
- Duplicate samples from each test medium and the control (without daphnias) just before the start of the test.
Duplicate samples from each test medium and the control after 96 hours.
All samples were taken from the approximate center of the aquaria without mixing of the test media, and were deep-frozen (at about -20 °C) immediately after sampling. - Vehicle:
- no
- Details on test solutions:
- The following nominal concentrations were tested: 4.6, 10, 22, 46 and 100 mg/L. Additionally, a control was tested in parallel.
The test medium of the highest nominal concentration of 100 mg/L was prepared by dissolving 50.0 mg of test item completely in 500 mL of test water using intense stirring for 15 minutes at room temperature. Adequate volumes of this test medium were diluted with test water to prepare the test media with the lower test item concentrations.
The test media were prepared just before introduction of the daphnids. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The study was performed with young daphnids of a clone of the species Daphnia magna Straus.
At the start of the test, the organisms used in the test were 6-24 hours old and were not first brood progeny. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 20 °C
- pH:
- 7.7 - 7.8
- Dissolved oxygen:
- 8.6 - 8.8 mg/L
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal: 4.6, 10, 22, 46 and 100 mg/L.
Measured: the nominal test item concentrations of 46 and 100mg/L were between 90 and 92 % of the nominal values at the start and the end of the test. - Details on test conditions:
- The test was performed in 100 mL glass beakers filled with 50 mL of test medium.
The beakers were covered with glass plates to reduce the loss of water and to avoid the entry of dust into the solutions.
At each test concentration and for the control, 20 daphnids were used divided into four replicates of five daphnids each.
Light conditions: A 16-hour light to 8-hour dark photopehod (with a 30 minute transition period). Light intensity during the light period was between
approximately 470 and 640 Lux.
No feeding. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No immobility of the test organisms was observed in the control and up to and including the test item concentration of 46 mg/L after 24 and 48 hours.
At the highest test concentration of 100 mg/L, the immobilization rate was 25 % and 50 % respectively after 24 and 48 hours. - Results with reference substance (positive control):
- The latest result of the positive control test in September 2005 (48-hour EC50: 0.81 mg/L, RCC Study No. A28102) showed that the toxic performance was valid and within the historical range of the RCC laboratory (from 1996 to 2005: 48-hour EC50: 0.53-1.1 mg/L).
- Reported statistics and error estimates:
- The 24-hour EC50 of the test item could not be calculated because the immobility rate was below 50 % up to the highest test concentration after 24 hours of exposure.
The 48-hour EC50 was determined directly from the raw data (the 95 % confidence limits could not be determined). The NOEC, EC0, and EC100 were also determined directly from the raw data. - Validity criteria fulfilled:
- yes
- Conclusions:
- The 24-hour EC0 was 46 mg/L while EC50 and EC100 were above 100 mg/L. The 48-hour EC50 was determined to be 100 mg/L while EC0 and were both 46 mg/L. The 48-hour EC100 were above 100 mg/L.
- Executive summary:
The acute toxicity of the test item FAT 40824/A to Daphnia magna was determined in a 48-hour static test according to the EU Commission Directive 92/69/EEC, Part C.2 (1992), and the OECD Guideline for Testing of Chemicals, No. 202 (2004) The nominal test item concentrations tested were 4.6, 10, 22, 46 and 100 mg/L. The test item FAT 40824/A was stable during the test period of 48 hours under the conditions of the test. The reported biological results were based on the nominal concentrations of the test item.
During the first 24 hours of the test, no immobility of the test organisms was observed in the control and up to and including the test item concentration of 46 mg/L. At the highest test concentration of 100 mg/L, the immobilization rate was 25% after 24 hours. The 24-hour EC0 was 46 mg/L. The 24-hour EC50 and EC100 of the test item and their 95% confidence limits could not be determined due to the low toxicity of the test item. However, these values were clearly above the test item concentration of 100 mg/L. After 48 hours of exposure, no immobility of the test organisms was observed in the control and up to and including the test item concentration of 46 mg/L. At the highest test concentration of 100 mg/L, the immobilization rate was 50% at the end of the test after 48 hours.
The 48-hour EC50 was determined to be 100 mg/L as the immobilization rate was exactly 50% at the test item concentration of 100 mg/L (the 95% confidence limits could not be determined). The 48-hour ECO and the 48-hour NOEC (highest concentration tested without toxic effects after 48 hours) of FAT 40824/A were both 46 mg/L since no immobilization was observed in the test organisms exposed to the test item at levels of up to and including this test concentration. The 48-hour EC100 could not be determined due to the low toxicity of the test item. However, the 48-hour EC100 was clearly above 100 mg/L.
Based on the test results, the 24-hour EC0 was 46 mg/L while EC50 and EC100 were above 100 mg/L. The 48-hour EC50 was determined to be 100 mg/L while EC0 and were both 46 mg/L. The 48-hour EC100 were above 100 mg/L.
Reference
During the first 24 hours of the test, no immobility of the test organisms was observed in the control and up to and including the test item concentration of 46 mg/L. At the highest test concentration of 100 mg/L, the immobilization rate was 25% after 24 hours.
The 24-hour EC0 was 46 mg/L. The 24-hour EC50 and EC100 of the test item and their 95% confidence limits could not be determined due to the low toxicity of the test item. However, these values were clearly above the test item concentration of 100 mg/L.
After 48 hours of exposure, no immobility of the test organisms was observed in the control and up to and including the test item concentration of 46 mg/L. At the highest test concentration of 100 mg/L, the immobilization rate was 50% at the end of the test after 48 hours.
The 48-hour EC50 was determined to be 100 mg/L as the immobilization rate was exactly 50% at the test item concentration of 100 mg/L (the 95% confidence limits could not be determined). The 48-hour ECO and the 48-hour NOEC (highest concentration tested without toxic effects after 48 hours) of FAT 40824/A were both 46 mg/L since no immobilization was observed in the test organisms exposed to the test item at levels of up to and including this test concentration. The 48-hour EC100 could not be determined due to the low toxicity of the test item. However, the 48-hour EC100 was clearly above 100 mg/L. Concentrations above 100 mg/L were not tested in accordance with the test guidelines.
No remarkable observations were made concerning the appearance of the test media. All test media were clear solutions colored by the test item throughout the entire test duration.
At the beginning and the end of the test period, the dissolved oxygen concentrations in the test media and the control were at least 8.6 mg/L. The pH values of the test media and the control were between 7.7 and 7.8 and the water temperature was 20 °C at the start and the end of the test.
Description of key information
The 24-hour EC0 was 46 mg/L while EC50 and EC100 were above 100 mg/L. The 48-hour EC50 was determined to be 100 mg/L while EC0 and were both 46 mg/L. The 48-hour EC100 were above 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
A GLP compliant acute toxicity of FAT 40824/A to Daphnia magna was determined in a 48-hour static test according to the EU Commission Directive 92/69/EEC, Part C.2 (1992), and the OECD Guideline for Testing of Chemicals, No. 202 (2004) The nominal test item concentrations tested were 4.6, 10, 22, 46 and 100 mg/L. The test item FAT 40824/A was stable during the test period of 48 hours under the conditions of the test. The reported biological results were based on the nominal concentrations of the test item.
During the first 24 hours of the test, no immobility of the test organisms was observed in the control and up to and including the test item concentration of 46 mg/L. At the highest test concentration of 100 mg/L, the immobilization rate was 25% after 24 hours. The 24-hour EC0 was 46 mg/L. The 24-hour EC50 and EC100 of the test item and their 95% confidence limits could not be determined due to the low toxicity of the test item. However, these values were clearly above the test item concentration of 100 mg/L. After 48 hours of exposure, no immobility of the test organisms was observed in the control and up to and including the test item concentration of 46 mg/L. At the highest test concentration of 100 mg/L, the immobilization rate was 50% at the end of the test after 48 hours.
The 48-hour EC50 was determined to be 100 mg/L as the immobilization rate was exactly 50% at the test item concentration of 100 mg/L (the 95% confidence limits could not be determined). The 48-hour EC0 and the 48-hour NOEC (highest concentration tested without toxic effects after 48 hours) of FAT 40824/A were both 46 mg/L since no immobilization was observed in the test organisms exposed to the test item at levels of up to and including this test concentration. The 48-hour EC100 could not be determined due to the low toxicity of the test item. However, the 48-hour EC100 was clearly above 100 mg/L.
Based on the test results, the 24-hour EC0 was 46 mg/L while EC50 and EC100 were above 100 mg/L. The 48-hour EC50 was determined to be 100 mg/L while EC0 and were both 46 mg/L. The 48-hour EC100 were above 100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.