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EC number: 212-782-2 | CAS number: 868-77-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 1996
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Source; Wako Pure Chemical Ind.,
Purity: 97.2% - Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- The medium dissolved by the material, and the examination field liquid of 2,000 mg/L is adjusted, in additon the filtration sterilisation was done with 0.45 um membrane filter.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Strain Number: Selenastrum capricornutum ATCC22662
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23.2 - 23.7 °C
- pH:
- 7.9 -10.4
- Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- 25.6, 64.0, 160, 400 and 1,000 mg/L - nominal
24.4, 61.3, 153, 401, and 1020 mg/L -TWM measured.
-Measured Concentrations (as mg/L): n. d. (<5.00) for control, 22.9-26.0 for the nominal concentration of 25.6, 57.1-65.7 for the nominal concentration of 64.0, 144-162 for the nominal concentration of 160, 391-410 for the nominal concentration of 400, and 994-1,020 for the nominal concentration of 1,000. - Details on test conditions:
- Test Conditions:
- Test Medium: OECD medium
- Exposure Vessel Type: 100 ml-medium in a 500 ml-erlenmeyer flask with a silicon cap which allow ventilation
- Stock Solutions Preparations and Stability: The medium dissolved by the material, and the examination field liquid of 2,000 mg/L is adjusted, in additon the filtration sterilization was done with 0.45 um membrane filter.
- Number of Replicates: Triplicate
- Initial Cell Number: 10000 cells/mL
- Water Temperature Range: 23.2 - 23.7 degree C
- Light Condition: 4200 - 4500 lux, continuous - Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 345 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 160 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- See Table 3 and Table 4.
- Reported statistics and error estimates:
- -Statistical Method:
a) Data Analysis: Least squares method for EC, Dunnett test for NOEC
b) Method of calculating mean measured concentrations: Time-weighted means - Validity criteria fulfilled:
- yes
- Conclusions:
- The 72 -hour EC50 and NOEC for Selenastrum capricornutum and based on growth rate was 345 mg/L and 160 mg/L, respectively.
- Executive summary:
A 72 -hour growth inhibition study with 2-hydroxyethyl methacrylate to Selenastrum capricornutum was conducted under static conditions with 3 replicates inoculated at 10,000 cells/mL per test concentration exposed to nominal test concentrations of 0 (control), 25.6, 64.0, 160, 400, and 1000 mg/L. Water samples were measured for analytical verification of test concentrations 0 and 72 hours of exposure and the resulting time-weighted mean measured concentrations were non-detectable (control), 24.4, 61.3, 153, 401, and 1020 mg/L. The 72 -hour EC50 and NOEC based on growth rate was 345 mg/L and 160 mg/L, respectively.
Reference
Table 4
Growth inhibition of Selenastrum capricornutum during 72 hrs exposure 2-Hydroxyethyl methacrylate
Nominal conc. (mg/l |
No. |
Area (*10.000) (0-72 hours) |
Inhibition (%) (0-72 hours) |
Control |
1 |
1860 |
- |
|
2 |
1460 |
- |
|
3 |
1530 |
- |
|
Average |
1620 |
|
25.6 |
1 |
1890 |
-17.0 |
|
2 |
1500 |
7.31 |
|
3 |
1860 |
-15.2 |
|
Average |
1750 |
-8.29 |
64.4 |
1 |
1830 |
-12.8 |
|
2 |
1050 |
35.1 |
|
3 |
1730 |
-6.88 |
|
Average |
1540 |
5.11 |
160 |
1 |
1670 |
-2.89 |
|
2 |
1580 |
2.54 |
|
3 |
1590 |
1.55 |
|
Average |
1610 |
0.398 |
400 |
1 |
756 |
53.3 |
|
2 |
661 |
59.2 |
|
3 |
707 |
56.3 |
|
Average |
708 |
56.3 |
1000 |
1 |
80.9 |
95.0 |
|
2 |
94.0 |
94.2 |
|
3 |
95.9 |
94.1 |
|
Average |
90.3 |
94.4 |
Table 4
Cell density of Selenastrum capricornutum during 72 hrs exposure 2-Hydroxyethyl methacrylate
Nominal conc. (mg/l |
Cell density (+ 10000 cell/ml) |
||||
No. |
0 hrs |
24 hrs |
48 hrs |
72 hrs |
|
Control |
1 |
1.0 |
4.8 |
29.4 |
91.4 |
|
2 |
1.0 |
4.0 |
25.7 |
67.8 |
|
3 |
1.0 |
5.0 |
23.9 |
74.9 |
|
Average |
1.0 |
4.6 |
26.3 |
78.0 |
|
SD |
0.0 |
0.5 |
2.8 |
12.2 |
25.6 |
1 |
1.0 |
3.8 |
29.9 |
95.4 |
|
2 |
1.0 |
3.7 |
25.6 |
71.5 |
|
3 |
1.0 |
3.9 |
28.8 |
94.9 |
|
Average |
1.0 |
3.8 |
28.1 |
87.3 |
|
SD |
0.0 |
0.1 |
2.2 |
13.7 |
64.4 |
1 |
1.0 |
3.4 |
26.9 |
96.5 |
|
2 |
1.0 |
3.6 |
21.7 |
41.9 |
|
3 |
1.0 |
3.2 |
27.2 |
88.5 |
|
Average |
1.0 |
3.4 |
25.2 |
75.6 |
|
SD |
0.0 |
0.2 |
3.1 |
29.5 |
160 |
1 |
1.0 |
3.4 |
27.9 |
81.1 |
|
2 |
1.0 |
2.6 |
26.6 |
78.0 |
|
3 |
1.0 |
2.9 |
24.3 |
83.4 |
|
Average |
1.0 |
3.0 |
26.2 |
80.9 |
|
SD |
0.0 |
0.4 |
1.8 |
2.7 |
400 |
1 |
1.0 |
2.1 |
15.0 |
33.8 |
|
2 |
1.0 |
1.9 |
13.9 |
28.3 |
|
3 |
1.0 |
1.8 |
12.5 |
35.2 |
|
Average |
1.0 |
2.0 |
13.8 |
31.4 |
|
SD |
0.0 |
0.1 |
1.3 |
3.6 |
1000 |
1 |
1.0 |
1.3 |
2.6 |
3.8 |
|
2 |
1.0 |
1.2 |
3.4 |
3.6 |
|
3 |
1.0 |
1.3 |
3.3 |
3.8 |
|
Average |
1.0 |
1.3 |
3.1 |
3.7 |
|
|
0.0 |
0.1 |
0.4 |
0.1 |
Description of key information
A freshwater algal toxicity test with Selenastrum capricornutum exposed to 2-hydroxyethyl methacrylate and conducted under GLPs and following OECD TG 201 resulted in a 72-hour growth rate EC50 (ErC50) and NOEC of 345 and 160 mg/L, respectively.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 345 mg/L
- EC10 or NOEC for freshwater algae:
- 160 mg/L
Additional information
One freshwater algal study with selenastrum capricornutum was available for 2-hydroxyethyl methacrylate and considered reliable for assessment of this endpoint. The study was conducted with selenastrum capricornutum and found to be of good quality and assigned a Klimisch score of one. The study was conducted for 72-hours, under static conditions, following OECD Test Guideline 201, and under GLPs. The study concluded that the 72-hour growth rate EC50 (ErC50) and NOEC was 345 and 160 mg/L, respectively and considered the key parameters for this endpoint.
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