Registration Dossier
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EC number: 266-113-4 | CAS number: 66071-94-1 Substance obtained by the partial removal of water from the liquid resulting from steeping corn in a water and sulphur dioxide solution which is allowed to ferment by the action of naturally occurring lactic acid-producing microorganisms.
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
In accordance with section 1 of Annex IX, the short-term toxicity testing on fish (section 9.1.3.) does not need to be conducted as corn steep liquor (CSL) consists of all-natural, water soluble components (crude proteins, amino acids, minerals, vitamins, reducing sugars, organic acids, enzymes and other elemental nutrients), that have been extracted by soaking corn in water, along with a very small amount of sulfurous acid (<0.01%). CSL has a long history of safe use as an added source of nutrition in animal feed, in fermentation processes, and in antibiotic production. As well, CSL is readily biodegradable. On this basis, CSL is not expected to cause adverse toxic effects to the environment. This expectation is supported by the demonstrated lack of acute fish toxicity of lactic acid - a major component of CSL.
Key value for chemical safety assessment
Additional information
Corn steep liquor (CSL) consists of all-natural, water soluble components (crude proteins, amino acids, minerals, vitamins, reducing sugars, organic acids, enzymes and other elemental nutrients), that have been extracted by soaking corn in water, along with a very small amount of sulfurous acid (<0.01%). CSL has a long history of safe use as an added source of nutrition in animal feed, in fermentation processes, and in antibiotic production. As well, CSL is readily biodegradable. On this basis, CSL is not expected to cause adverse toxic effects to the environment.
While no studies are available on CSL, three studies addressing short-term toxicity of lactic acid, a major component of CSL, to fish are presented in the dossier.
Acute toxicity to the freshwater fish Brachydanio rerio (new name: Danio rerio) (Zebrafish) was assessed according to OECD guideline 203 (Fish, acute toxicity test) under Good Laboratory Practices (GLP) in 1992 at Netherlands Organisation for Applied Scientific Research – TNO. The fish were exposed to lactic acid under semi-static conditions for 96 hours. The 96–hour LC50 was determined to be 320 mg/L.
Acute toxicity to the freshwater fish Lepomis macrochirus (Bluegill) was assessed according to Committee on methods for Toxicity Tests with Aquatic Organisms. 1975. Methods for acute toxicity tests with fish, macro invertebrates and amphibians. Environmental Protection Agency, Ecological Research Series EPA 660/3-75-009, April 1975 according GLP in 1984 at Analytical Biochemistry Laboratories Inc. Columbia, MO. The fish were exposed to lactic acid under static conditions for 96 hours. The 96–hour LC50 was determined to be 130 mg/L.
Acute toxicity to the freshwater fish Salmo gairdneri (Rainbow trout) was assessed according to OECD guideline 203 (Fish, acute toxicity test) according GLP in 1984 at Analytical Biochemistry Laboratories Inc., Columbia, MO. The fish were exposed to lactic acid under static conditions for 96 hours. The 96–hour LC50 was determined to be 130 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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