Linseed oil, polymd.

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March - May 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

not according to GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

(No analytical confirmation of concentrations of test compound in test solutions)

GLP compliance:

no

Test material

Test material information

1

Reference substance name:

3-[(8-{5-hexyl-6-[(2Z)-oct-2-en-1-yl]-2-(8-{2-[(7Z,10Z,13Z)-octadeca-7,10,13-trienoyloxy]-3-[(9Z,12Z)-octadeca-9,12-dienoyloxy]propoxy}-8-oxooctyl)cyclohex-3-en-1-yl}octanoyl)oxy]-2-[(7E,10E,13Z)-octadeca-7,10,13-trienoyloxy]propyl (9Z,12Z,15Z)-octadeca-9,12,15-trienoate

EC Number:

614-114-9

Cas Number:

67746-08-1

Molecular formula:

Not applicable for UVCB

IUPAC Name:

3-[(8-{5-hexyl-6-[(2Z)-oct-2-en-1-yl]-2-(8-{2-[(7Z,10Z,13Z)-octadeca-7,10,13-trienoyloxy]-3-[(9Z,12Z)-octadeca-9,12-dienoyloxy]propoxy}-8-oxooctyl)cyclohex-3-en-1-yl}octanoyl)oxy]-2-[(7E,10E,13Z)-octadeca-7,10,13-trienoyloxy]propyl (9Z,12Z,15Z)-octadeca-9,12,15-trienoate

Details on test material:

- Name of test material (as cited in study report): Bodied linseed oil, 800 P. (Leinöl-standöl, 800 P)
- Analytical purity: N/A; treated as 100% pure
- Lot/batch No.: No data

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

Not applicable

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solutions were prepared by mechanical dissolving (Ultra-Turrax) of the test substance in the required volume of deionised water. This stock solution was transferred into the test medium of one glass tank 2 hours before the start of the test (i.e. the addition of the fish, t=0 h). In the meantime, until the fish were transferred, the contents of each glass tank were mixed thoroughly using a mechanical stirrer in order to facilitate the dissolving and/or dispersing of the test sample.
- Eluate: not relevant
- Differential loading: not relevant
- Controls: controls were untreated
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not relevant
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)):not relevant
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The following was reported: "In the preparation of the different test batches …. It could be observed that charging the test medium with the test substance led to a flocky dispersion slowly settling up in the course of the test period.”

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Source: West Aquarium GmbH, D-3422 Bad Lauterberg, Germany
- Age at study initiation: about 2 months
- Length at study initiation: Average total length: 2.0 ± 1.0 cm
- Weight at study initiation (mean and range, SD): no data
- Method of breeding: no data
- Feeding during test: 24 hours before the test was started, feeding of the fish was stopped

ACCLIMATION
- Acclimation period: 2 weeks
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Tetra Min Hauptfutter
- Feeding frequency: Twice daily
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not applicable

Test conditions

Hardness:

Not measured during study

Test temperature:

Overall range during study: 20.7 - 22.1 ºC

pH:

7.5 (measured during study)

Dissolved oxygen:

Overall range during study: 100% - >86%

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal: 0; 100 and 1000 mg/L
The nominal concentrations were not verified.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass tank with dimensions: length 300 mm, width 200 mm and height 220 mm
- Type: open
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water was prepared in accordance with OECD 203;
deionised water used for its preparation was taken from the delivery system of the laboratory.
- Alkalinity: 0.8 mmol/L
- Ca/Mg ratio: 4:1
- Intervals of water quality measurement: pH, oxygen and temperature were measured at 0, 1, 24, 48, 72 and 96 hours.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod and light intensity: no data

EFFACT PARAMETERS MEASURED
Mortality and fish behavior were observed at 0, 1, 24, 48, 72 and 96 hours

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Highest testconcentration. Based on slowly settling dispersions, no chemical analysis of test substance concentrations.

Effect concentrations 2

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Based on approximate solubility limit of test substance in test medium

Details on results:

- Mortality in test mediums: 0%
- Mortality of control: 0%

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Not applicable

Any other information on results incl. tables

Sublethal observations / clinical signs:

The reported LC0 was 1000 mg/L. The test solutions were flocky dispersions, which were observed to be slowly settling up in the course of the test period. The actual concentrations were not confirmed by chemical analysis. All tested concentrations were far above the solubility limit of the test material in water, which is <1 mg/L. The solubility limit in reconstituted test medium may be even less than that in water. Due to the fact that the test solutions were slowly settling dispersions far in excess of the solubility limit, the concentrations of which were not analytically determined, there is no information about the actual concentrations to which the test fish were exposed during the study. However, due to the method of preparation of the test solutions (thorough mixing of stock using Ultra-Turrax mixer, addition of stock to test water, followed by 2 hours of thorough mixing using a mechanical stirrer), it is sufficiently certain that the solubility limit of the test substance in the test water was achieved in all test solutions. Since at 1000 mg/L no fish mortality occurred, the conclusion of the study is that the LC50 is in excess of the solubility limit.

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Fish acute toxicity test in Brachydanio rerio according to OECD 203: LC50 in excess of the solubility limit (hence in excess of about 1 mg/L)