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EC number: 241-774-1 | CAS number: 17796-82-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-(cyclohexylthio)phthalimide
- EC Number:
- 241-774-1
- EC Name:
- N-(cyclohexylthio)phthalimide
- Cas Number:
- 17796-82-6
- Molecular formula:
- C14H15NO2S
- IUPAC Name:
- N-(cyclohexylthio)phthalimide
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- The inoculum contained a mixed population of microorganisms obtained from the secondary effluent of the wastewater treatment plant of Pardubice processing predominantly municipal sewage.
The fresh collected wastewater was filtered through paper filter. COD was determined and wastewater was aerated till using.
The mineral medium was strongly aerated for 20 min. and let standing for 20 h at the test temperature. The volume of wastewater for inoculation of mineral medium was chosen 1.6 mL per 1 L of medium ( COD of waste water: 121 mg/L)
30 L aerated mineral medium was prepared for the test and before the beginning of the test it was inoculated with 48 mL of modified wastewater. The pH value of medium: 7.4. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 3.46 mg/L
- Based on:
- COD
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Solutions and chemicals: The deionised water with conductivity less then 5 uS/cm was used for the preparation of all solutions. The same bath of water was used for each set of the experiments. Analytical grade reagents were used.
Stoc solution for the preparation of mineral medium:
solution a) KH2PO4 8.50 g, K2HPO4 21.75 g, Na2HPO4.2H2O 33.4 g, NH4Cl 0.50 g, water up to the volume of 1000 mL.
- pH:7.4
- pH adjusted: yes
solution b) MgSO4.7H2O 22.5 g in 1000 mL of water
solution c) CaCl2 27.5 g in 1000 mL of water
solution d) FeCl3.6H2O 0.25 g in 1000 mL of water
- Composition of medium: preparation of 1 L of mineral medium. 1 mL of solution a), b), c) and d) were mixed with apprex. 500 mL of water, the mixture was replenished with water up to 1000 mL. The solution was prepared from aerated water and it was left to stay one day at laboratory temperature.
- Test temperature: 20 ± 1.0°C
- Continuous darkness: yes
- Exposition time: 28 days
TEST SYSTEM
-oxygen vesel with glass stopper, volume of approx. 280 mL
-large glass bottles of 5 and 10 L volume for the preparation and inoculation of medium
-closed thermostat
-Ph meter WTW inoLab Level 2
-equipment for the determination of dillolved oxygen
-equipment for the determination of nitrite
-equipment for the determination of nitrate
-volumetric flasks, flasks, beakers, pipettes...
Series of bottles for the test:
- 2x9 bottles with test substance and inoculated medium
- 9 bottles with test substance and inoculated medium for determination of oxidized nitrogen forms
- 2x9 bottles with test substance and inoculated medium for the check of inoculum activity
- 2x5 bottles with test and reference substance mixture and inoculated medium for the toxicity test
- 2x9 bottles with inoculated medium only for the blank determination
The bottles were lined up and placed into thermosta.
SAMPLING
- Sampling frequency:0 -3rd-7th-10th-14th-17th-21st-24th-28th day
- Sampling method: appropriate bottles from each series were taken off and they were analysed for dissolved oxygen.
- Sample storage before analysis: enclosed in a full closed bottles, in the dark, at constant temperature
- Other:
CONTROL AND BLANK SYSTEM
- Inoculum blank: The inoculated mineral medium without test substance was used for the blank determination.
- reference substance: The stock solution of sodium benzoate was prepared in concentration 1.0000 g/L of water. From this solution the 12.6 mL (2.1 mL/L) were transferred to a large glass bottle and completed with the inoculated medium to the volume of 6 L.
The solution prepared in this way contained 2.10 mg/L of sodium benzoate, and had COD 3.45 mg/L. The pH value of solution: 7.4.
- Toxicity control:4.7 mL of dispersion of test substance were dosen into individual bottles and completed with inoculated solution of sodium benzoate of concentration 2.10 mg/L. mixtures prepared in this way contained COD of test substance 3.46 mg/L and COD of reference substance 3.40 mg/L. The pH value of mixtures: 7.4.
- Other:
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- Measurement
The zero-time bottles were analysed immediately for dissolved oxygen by the Winkler method.
At the 3rd, 7th, 10th, 14th, 17th, 21st, 24th, 28th day of test the appropriate bottles from each series were taken off and they were analysed for dissolved oxygen.
The determination of nitrates and nitrites was caried out in parallel bottles in these time intervals by means of spectrometric methods.
Evaluation
The oxygen consumption and percentage of the biodegradation of test substance for each test interval and each bottle were calculated. In time intervals when the increase of oxidized nitrogen forms was detected, the correction of oxygen consumption for nitrification was carried out.
The evaluation of the reference substance and of the test and reference substance substance mixture was done by the same way, but withouth the correction of nitrification. All the calculations were related to the initial values of COD of the test and reference substance.
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 16
- Sampling time:
- 28 d
Any other information on results incl. tables
Determination of dissolved oxygen concentration in the main test
|
Conc. C |
|
Disolved oxygen (mg/L) after period (days) |
||||||||
0 |
3 |
7 |
10 |
14 |
17 |
21 |
24 |
28 |
|||
Test substance |
Cz |
1 |
8.57 |
8.55 |
8.39 |
8.53 |
8.49 |
8.45 |
8.12 |
8.07 |
7.96 |
2 |
8.57 |
8.58 |
8.39 |
8.56 |
8.49 |
8.43 |
8.12 |
8.10 |
8.02 |
||
average |
8.57 |
8.57 |
8.39 |
8.55 |
8.49 |
8.44 |
8.12 |
8.09 |
7.99 |
||
Reference substance |
Cr |
1 |
8.55 |
6.72 |
6.47 |
6.59 |
6.33 |
6.25 |
6.07 |
6.02 |
5.92 |
2 |
8.52 |
6.75 |
6.50 |
6.53 |
6.33 |
6.25 |
6.02 |
6.02 |
5.97 |
||
average |
8.54 |
6.74 |
6.49 |
6.56 |
6.33 |
6.25 |
6.05 |
6.02 |
5.95 |
||
Blank experiment |
Co |
1 |
8.58 |
8.57 |
8.42 |
8.65 |
8.59 |
8.51 |
8.55 |
8.58 |
8.49 |
2 |
8.55 |
8.57 |
8.42 |
8.65 |
8.59 |
8.54 |
8.55 |
8.56 |
8.50 |
||
average |
8.57 |
8.57 |
8.42 |
8.65 |
8.59 |
8.53 |
8.55 |
8.57 |
8.50 |
Biodegradation of test and reference substance in the main test
|
|
Biodegradation (%) after period (days) |
||||||||
0 |
3 |
7 |
10 |
14 |
17 |
21 |
24 |
28 |
||
Test substance |
1 |
0 |
1.7 |
2.0 |
4.6 |
4.0 |
3.5 |
13.6 |
14.5 |
16.8 |
2 |
0 |
0.9 |
2.0 |
3.8 |
4.0 |
4.0 |
13.6 |
13.6 |
15.0 |
|
average |
0 |
1.3 |
2.0 |
4.2 |
4.0 |
3.8 |
13.6 |
14.1 |
15.6 |
|
Reference substance |
1 |
0 |
53.0 |
55.9 |
59.1 |
64.9 |
65.5 |
71.3 |
73.3 |
74.2 |
2 |
0 |
51.3 |
54.2 |
60.0 |
64.1 |
64.6 |
71.9 |
72.5 |
71.9 |
|
average |
0 |
52.2 |
55.1 |
59.6 |
64.5 |
65.1 |
71.6 |
72.9 |
73.1 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not ready biodegradable
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