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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 25/09/2014 to 24/10/2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Study conducted according OECD guideline and in compliance with GPL
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- The temperature during the test was at 19 °C for 1.5 days (2014-09-27 to 2014-09-28). This deviation was considered to have no impact on the quality and integrity of the study.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 1720
- Expiration date of the lot/batch: 08/10/2014
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature, protected from light, in the tightly closed original container under nitrogen
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: The test item was heated to 40 °C until homogeneity was reached before further use. The test item was treated with ultrasound for 10 minutes at room temperature.
OTHER SPECIFICS:
CAS No. 1119235-20-9
IUPAC Chemical Name Heat-treated distillate of avocado oil, Persea gratissima, Lauraceae
Type of substance UVCB substance
Chemical characterisation Fatty acids, glycerides, unsaponifiables
Main components Total sterol content : 1.4% / Total furanic compounds content : 26.7% / Total triols 1,2,4 content : 6.7% / Unsaponifiable content : 34.8%
Appearance Brown to orange coloured semi-liquid product which can be partially crystallized
Molecular weight Mean value 385 g/mol
Water solubility Insoluble
TOC* 63.5% - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
Municipal sewage treatment plant, 31137 Hildesheim, Germany. Activated sludge from the sewage plant at Hildesheim is well suited as it receives predominantly municipal sewage and hardly any industrial chemical waste.
- Pretreatment:
The activated sludge was washed twice with chlorine free tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 20 hours. Thereafter the sludge was homogenized with a blender. After sedimentation the supernatant was decanted and maintained in an aerobic condition by aeration with CO2 free air until test start. Ten mL/L of this mixture were used to initiate inoculation.
- Initial cell/biomass concentration: Approx. 107 - 108 CFU/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 15 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral salts medium acc. to OECD 301 B / CO2 Evolution Test
- Solubilising agent (type and concentration if used): Based on the results of pre-investigations performed prior to this test and based on the recommendations of relevant guidance from ECHA and ECETOC/UBA/EA for poorly water soluble substances (please refer to the point ‘Literature’), humic acid was used in additional incubation vessels in order to enhance the bioavailability of CAE.
- Test temperature: Nominal 22 ± 2 °C, actually measured 19 – 24 °C
- pH adjusted: no
- Aeration of dilution water: 30 - 100 mL/min
- Continuous darkness: yes. Low light conditions (brown glass bottles: test media excluded from light)
The following incubation vessels were prepared:
- two for the test item concentration (P1, P2)
- one for the functional control (R1)
- two for the inoculum control (C1, C2)
- one for the toxicity control (T1)
- two for the test item concentration with humic acid (P1HA, P2HA)
- two for the humic acid control (C1HA, C2HA)
TEST SYSTEM
- Method used to create aerobic conditions: The vessels were aerated for 24 h with CO2 free air.
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: After 24 h the CO2 adsorption vessels were connected to the air outlets of the incubation vessels via a series of 3 gas wash bottles, each containing 100 mL of a 0.0125 mol/L Ba(OH)2 solution.
- Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 100
- Sampling time:
- 28 d
- Remarks on result:
- other: test item replicates with and without humic acid
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 60
- Sampling time:
- 8 d
- Remarks on result:
- other: test item replicates without humic acid
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 60
- Sampling time:
- 9 d
- Remarks on result:
- other: test item replicates with humic acid
- Details on results:
- The adaptation phase of the functional control changed after 2 days into the degradation phase (degradation >10%). The course of the degradation was rapid and the functional control reached the pass level of 60% within 6 days and a biodegradation of 100% after 28 days. The validity criterion degradation > 60% after 14 days was fulfilled.
In the toxicity control containing both test and reference item a biodegradation of 62% was determined within 7 days and it came to 96% after 28 days. The biodegradation of the reference item was not inhibited by the test item in the toxicity control.
For the test item replicates without humic acid, the mean 10% level (beginning of biodegradation) was reached on day 2. The 60% pass level was reached by the mean of replicates on day 8 and the 10-day-window was fulfilled. The mean biodegradation after 28 days was 100%. For the test item replicates with humic acid, the mean 10% level (beginning of biodegradation) was reached on day 3. The 60% pass level was reached by the mean of replicates within 9 days and the 10-day-window was fulfilled. The mean biodegradation after 28 days was 100%. - Results with reference substance:
- The adaptation phase of the functional control changed after 2 days into the degradation phase (degradation > 10%). The course of the degradation was rapid and the functional control reached the pass level of 60% within 6 days and a biodegradation of 100% after 28 days. The validity criterion degradation > 60% after 14 days was fulfilled.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Under the test conditions the test item CAE is classified as readily biodegradable in the 10-day-window and within 28 days.
- Executive summary:
The ready biodegradability of the test item CAE (Batch No.1720) was determined with a non-adapted activated sludge over a test period of 28 days in the Modified Sturm Test. The study was conducted from 2014-09-25 to 2014-10-24, according to OECD 301 B at Dr.U.Noack-Laboratorien.
The Total Organic Content (TOC) of CAE was determined at an external test facility to be 63.5% acc. to ISO 10694:1995. The test item was tested at a concentration of 15 mg/L with 2 replicates, corresponding to a carbon content (TOC) of 9.53 mg C/L in the test vessels. Based on the results of pre-investigations performed prior to this test and based on the recommendations of relevant guidance from ECHA and ECETOC/UBA/EA for poorly water soluble substances, humic acid was used in additional incubation vessels (2 replicates) in order to enhance the bioavailability of CAE.The test vessels were incubated at low light conditions and at a temperature of 19 – 24 °C.
The biodegradation of the test item was followed by titrimetric analysis of the quantity of CO2 produced by the respiration of bacteria. The degradation was stopped on day 28 by acidification of the test solutions. The last titration was made on day 29, after residual CO2 had been purged from the test solutions over a period of 24 hours. The percentage CO2 production was calculated in relation to the theoretical CO2 production (ThCO2) of the test item. The biodegradation was calculated for each titration time.
To check the activity of the test system, sodium benzoate was used as functional control. The percentage degradation of the functional control reached the pass level of 60% within 6 days and a biodegradation of 100% after 28 days.
In the toxicity control containing both test and reference item a biodegradation of 60% was determined within 7 days and it came to 96% after 28 days. Hence the biodegradation of the reference item was not inhibited by the test itemin the toxicity control.
For the test item replicates without humic acid, the mean 10% level (beginning of biodegradation) was reached on day 2. The 60% pass level was reached by the mean of replicates on day 8 and the 10-day-window was fulfilled. The mean biodegradation after 28 days was 100%. For the test item replicates with humic acid, the mean 10% level (beginning of biodegradation) was reached on day 3. The 60% pass level was reached by the mean of replicates within 9 days and the 10-day-window was fulfilled. The mean biodegradation after 28 days was 100%.
Under the test conditions the test item is classified as readily biodegradable in the 10-day-window and within 28 days.
Reference
Table 1: CO2-Production and Biodegradation after 28 Days
CO2-Production |
Functional |
Test Item |
Test Item |
Toxicity Control |
||
Control |
with humic acid |
test item + |
||||
reference item |
1 |
2 |
1 |
2 |
||
Net [mg/3 L] |
128.7 |
106.2 |
126.3 |
101.2 |
120.6 |
222.6 |
[mg/L] |
42.9 |
35.4 |
42.1 |
33.7 |
40.2 |
74.2 |
Theor. [mg/3 L] |
127.8 |
104.9 |
104.9 |
232.7 |
||
[mg/L] |
42.6 |
35.0 |
35.0 |
77.6 |
||
Degradation [%] |
100 |
100 |
100 |
96 |
100 |
96 |
Table2: CO2-Production and Biodegradation in the Inoculum Control, the Control and the Toxicity Control
Study Day |
Date |
Inoculum |
Inoculum |
Functional Control |
Toxicity Control |
||||
|
|
[mg CO2/3 L] |
[mg CO2/3 L] |
[mg CO2/3 L] |
Degr. |
[mg CO2/3 L] |
Degr. |
||
|
|
mv |
mv |
Gross |
Net Sum |
[%] |
Gross |
Net Sum |
[%] |
1 |
2014-09-26 |
4.1 |
3.2 |
5.8 |
1.7 |
1 |
4.7 |
0.6 |
0 |
4 |
2014-09-29 |
7.1 |
7.1 |
56.3 |
50.9 |
40 |
56.5 |
50.0 |
21 |
6 |
2014-10-01 |
4.9 |
5.3 |
44.3 |
90.3 |
71 |
56.5 |
101.6 |
44 |
7 |
2014-10-02 |
2.9 |
2.9 |
19.9 |
107.3 |
84 |
45.3 |
144.0 |
62 |
11 |
2014-10-06 |
8.0 |
7.9 |
17.4 |
116.7 |
91 |
41.3 |
177.3 |
76 |
14 |
2014-10-09 |
6.5 |
6.6 |
10.1 |
120.3 |
94 |
25.9 |
196.7 |
85 |
18 |
2014-10-13 |
6.7 |
8.2 |
11.0 |
124.6 |
97 |
22.6 |
212.6 |
91 |
21 |
2014-10-16 |
7.0 |
6.6 |
10.1 |
127.7 |
100 |
12.1 |
217.7 |
94 |
25 |
2014-10-20 |
7.3 |
6.9 |
8.1 |
128.5 |
100 |
9.9 |
220.3 |
95 |
28 |
2014-10-23 |
6.7 |
6.0 |
6.6 |
128.5 |
100 |
7.6 |
221.2 |
95 |
29* |
2014-10-24 |
9.6 |
10.3 |
9.8 |
128.7 |
100 |
10.6 |
222.6 |
96 |
Degr. = degradation mv = mean value *) results of last two gas wash bottles
Table3: CO2-Production and Biodegradation in the Inoculum Control and Test Item Replicates (without humic acid)
Study Day Day |
Date |
Inoculum |
Test Item |
||||||
Replicate 1 |
Replicate 2 |
||||||||
|
|
[mg CO2/3 L] |
[mg CO2/3 L] |
Degr. |
[mg CO2/3 L] |
Degr. |
|||
|
|
mv |
Gross |
Net Sum |
[%] |
Gross |
Net Sum |
[%] |
|
1 |
2014-09-26 |
4.1 |
3.4 |
0.0 |
0 |
3.9 |
0.0 |
0 |
|
4 |
2014-09-29 |
7.1 |
38.3 |
31.2 |
30 |
42.4 |
35.3 |
34 |
|
6 |
2014-10-01 |
4.9 |
26.1 |
52.4 |
50 |
24.0 |
54.4 |
52 |
|
7 |
2014-10-02 |
2.9 |
12.2 |
61.7 |
59 |
9.6 |
61.1 |
58 |
|
11 |
2014-10-06 |
8.0 |
28.2 |
81.9 |
78 |
35.2 |
88.3 |
84 |
|
14 |
2014-10-09 |
6.5 |
16.8 |
92.2 |
88 |
20.8 |
102.6 |
98 |
|
18 |
2014-10-13 |
6.7 |
14.1 |
99.6 |
95 |
16.7 |
112.6 |
100 |
|
21 |
2014-10-16 |
7.0 |
11.4 |
104.0 |
99 |
12.5 |
118.1 |
100 |
|
25 |
2014-10-20 |
7.3 |
7.7 |
104.4 |
100 |
9.4 |
120.2 |
100 |
|
28 |
2014-10-23 |
6.7 |
6.8 |
104.5 |
100 |
8.1 |
121.6 |
100 |
|
29* |
2014-10-24 |
9.6 |
11.3 |
106.2 |
100 |
14.3 |
126.3 |
100 |
|
Degr. = degradation mv = mean value *) results of last two gas wash bottles
Table 4: CO2-Production and Biodegradation in the Inoculum Control and Test Item Replicates (with humic acid)
Study Day |
Date |
Inoculum |
Test Item |
||||||
Replicate 1 |
Replicate 2 |
||||||||
|
|
[mg CO2/3 L] |
[mg CO2/3 L] |
Degr. |
[mg CO2/3 L] |
Degr. |
|||
|
|
mv |
Gross |
Net Sum |
[%] |
Gross |
Net Sum |
[%] |
|
1 |
2014-09-26 |
3.2 |
3.4 |
0.2 |
0 |
4.1 |
0.9 |
1 |
|
4 |
2014-09-29 |
7.1 |
30.9 |
24.0 |
23 |
30.7 |
24.5 |
23 |
|
6 |
2014-10-01 |
5.3 |
16.7 |
35.4 |
34 |
26.8 |
46.0 |
44 |
|
7 |
2014-10-02 |
2.9 |
12.7 |
45.2 |
43 |
13.3 |
56.4 |
54 |
|
11 |
2014-10-06 |
7.9 |
32.9 |
70.2 |
67 |
32.7 |
81.2 |
77 |
|
14 |
2014-10-09 |
6.6 |
18.9 |
82.5 |
79 |
21.7 |
96.3 |
92 |
|
18 |
2014-10-13 |
8.2 |
17.2 |
91.5 |
87 |
18.7 |
106.8 |
100 |
|
21 |
2014-10-16 |
6.6 |
10.5 |
95.4 |
91 |
12.9 |
113.1 |
100 |
|
25 |
2014-10-20 |
6.9 |
9.4 |
97.9 |
93 |
10.5 |
116.7 |
100 |
|
28 |
2014-10-23 |
6.0 |
7.2 |
99.1 |
94 |
8.7 |
119.4 |
100 |
|
29* |
2014-10-24 |
10.3 |
12.4 |
101.2 |
96 |
11.5 |
120.6 |
100 |
|
Degr. = degradation mv = mean value *) results of last two gas wash bottles
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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