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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
other information
Study period:
October 11 - October 31 2006
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: according to guideline under GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
92/69/EEC, Part C, Method: 2
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
4-(4-nitrophenyl)morpholin-3-one
EC Number:
610-199-1
Cas Number:
446292-04-2
Molecular formula:
C10 H10 N2 O4
IUPAC Name:
4-(4-nitrophenyl)morpholin-3-one
Details on test material:
- Name of test material (as cited in study report): 4-(4-Nitrophenyl)-3-morpholinon
- Analytical purity: 97,5 %
- Lot/batch No.: BXR2LPC

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 96.5
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

No toxic effects against daphnia at a test concentration of 100 mg/l.

The results are expressed in terms of nominal. Recovery rates correspond to 97,4% of nominal values at 0 hours, and 96,5% of nominal values at 48 hours, respectively. The temperature was 20.6°C, O2 concentration 8.4 mg/L and the pH value was 8.1.

Applicant's summary and conclusion

Conclusions:
4-(4-Nitrophenyl)-3-morpholinon shows no toxic effects against daphnia at a test concentration of 100 mg/I.
Executive summary:

A study was performed to access the acute toxicity of 4-(4-Nitrophenyl)-3-morpholinon to Daphnia magna STRAUS under static conditions. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 2 "Acute toxicity füor Daphnia" which is in most parts equivalent to the OECD guideline for Testing of Chemicals No. 202 "Daphnia sp., Acute Immobilisation Test and Reproduction Test, Part 1 - The 24h EC50 Acute Immobilisation Test". The Daphnia were exposed to a limit test concentration of nominally 100 mg/l of 4-(4-Nitrophenyl)-3-morpholinon dissolved in Water. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer and a folder filter.

Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48h of exposure. The following values were determined:

 Time [h]  EC 50 [mg/l]
 24  > 100
 48  > 100

No toxic effects against daphnia at a test concentration of 100 mg/l.

The results are expressed in terms of nominal. Recovery rates correspond to 97,4% of nominal values at 0 hours, and 96,5% of nominal values at 48 hours, respectively