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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1983-03-10 to 1983-04-11
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No GLP mentioned. Basic data are given in the study report summary.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report date:
1983

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
other: Standard Japanese test methods (in according with the Industrial Safety and Health Act)
Deviations:
not specified
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
p-phenylbenzaldehyde
EC Number:
221-742-3
EC Name:
p-phenylbenzaldehyde
Cas Number:
3218-36-8
Molecular formula:
C13H10O
IUPAC Name:
[1,1'-biphenyl]-4-carbaldehyde
Test material form:
not specified

Method

Species / strain
Species / strain / cell type:
S. typhimurium, other: TA 98, TA100, TA 1535, TA 1537; TA1538 and E. Coli WP2uvrA
Metabolic activation:
with and without
Metabolic activation system:
S9 mix
Test concentrations with justification for top dose:
Six doses: 1 - 500 µg/plate or 0.5 - 100 µg/plate
The tests of TA 98 without S9-mix in Salmonella typhimurium were conducted in a concentration of 0.1 - 50 µg/plate.
Vehicle / solvent:
No data
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Details on test system and experimental conditions:
No data
Evaluation criteria:
No data
Statistics:
No data

Results and discussion

Test results
Species / strain:
S. typhimurium, other: TA 98, TA100, TA 1535, TA 1537; TA1538 and E. Coli WP2uvrA
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
Regarding the above concentration, the growth inhibition of Salmonella typhimurium was confirmed.
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Remarks on result:
other: all strains/cell types tested

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

The test substance is non mutagenic in the bacterial reverse mutation test, both in the presence and absence of a metabolizing system (S9-mix).
Executive summary:

The test substance had been tested for reverse mutation in Salmonella typhimurium TA 98, TA100, TA 1535, TA 1537; TA1538 and Escherichia coli WP2uvrA with and without exogenous metabolic activation at six doses: 1 – 500 µg/plate or 0.5 – 100 µg/plate. In addition, the tests of TA 98 without S9-mix in Salmonella typhimurium were conducted in a concentration of 0.1 – 50 µg/plate. The tests were negative, both in the presence and absence of a metabolizing system (S9-mix).