Disodium 3-[(5-chloro-2-phenoxyphenyl)azo]-4-hydroxy-5-[[(p-tolyl)sulphonyl]amino]naphthalene-2,7-disulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From August 31 to November 15, 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Composite samples of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 48 hours exposure and kept at - 18°C to - 22°C until analysis.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Stain: Stauss 1820- Source: CIBA-GEYGY- Feeding: none during the test- Breeding: Cultures of daphnia are maintained in glass vessels containing approx. 2.5 1 of reconstituted water at 20 ± 1 °C (water is renewed partially thrice weekly). At each renewal the daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantities that the food is consumed after 24h.

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

Reconstituted water containing65 mg NaHC03294 mg CaCl2 • 2 H20123 mg MgS04 • 7 H206 mg KClin 1000 ml bidistilled water.Total hardness : 240 mg CaC03/l.

Test temperature:

20 ± 1°C (measured after 24 and 48 h)

pH:

7.8 (measured after 24 and 48 h)

Dissolved oxygen:

The water was aerated with clean air for at least 24 hours before use.96% (measured after 24 and 48 h)

Nominal and measured concentrations:

- STOCK SOLUTION: 200.5 mg test substance were mixed and made up to 2000 ml with water.- NOMINAL: 2.0,4.4, 9.6,21,46 and 100 mg test substance- BLANK: water

Details on test conditions:

TEST SYSTEM- No. of organisms per vessel: 20 per concentration and control- No. of vessels per concentration (replicates): 4 replicates of 5 daphnia each- Pre-treatment: 24 hours before the begin of the exposure reproductive daphnia are separated from the young by sieving all individuals through a 800 um sieve. This operation is repeated immediately before the exposure and the young (0-24h of age) are retained for the test.OTHER TEST CONDITIONS- Lighting: Fluorescent light, 16 hours daily approx. 1500 lux 48OBSERVATIONImmobilizations were recorded after 24 and 48 hours exposure

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Remarks on result:

other: 95% confidence limit

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

104 mg/L

Nominal / measured:

nominal

Remarks on result:

other: 95% confidence limit

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Results with reference substance (positive control):

- Immobilization in blank: 5%

Reported statistics and error estimates:

The LC-50 values were calculated according to BERKSON, JASA 48 (1953), 569-599.EC-values were graphically determined on gausso-logarithmic probability paper

Conclusions:

EC 50 (48 h) calculated: 104 mg/L95 % confidence limit: 88-153 mg/LEC 50 (24 h) calculated: > 100 mg/LNOEC (48 h): 46 mg/LEC 0 (48 h): 46 mg/LEC 100 (48h): > 100 mg/L

Executive summary:

The determination on ecotoxicity of the substance was determined in an Acute toxicity for Daphnia, test according to the guideline 92/69/EEC C.2. The test has been performed in compliance with the Procedures and Principles of Good Laboratory Practice. The test substance was tested on Daphnia magna Straus 1820 for 48 hours.

20 daphnids (0-24 hours old) were exposed to nominal concentrations of 2.0, 4.4, 9.6,21,46 and 100 mg test substance respectively.

Results are:

EC 50 (48 h) calculated: 104 mg/L

95 % confidence limit: 88-153 mg/L

EC 50 (24 h) calculated: > 100 mg/L

NOEC (48 h): 46 mg/L

EC 0 (48 h): 46 mg/L

EC 100 (48h): > 100 mg/L

Description of key information

EC 50 (48 h) calculated: 104 mg/l
EC 50 (24 h) calculated: > 100 mg/l
NOEC (48 h): 46 mg/l
EC 0 (48 h): 46 mg/l
EC 100 (48h): > 100 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

104 mg/L

Additional information

The determination on ecotoxicity of the substance to be registered was determined in an acute test on Daphnia, test according to the guideline 92/69/EEC C.2. The test has been performed in compliance with the Procedures and Principles of Good Laboratory Practice. The test substance was tested on Daphnia magna Straus 1820 for 48 hours.

20 daphnids (0-24 hours old) were exposed to nominal concentrations of 2.0, 4.4, 9.6,21,46 and 100 mg test substance respectively.

Results are:

EC 50 (48 h) calculated: 104 mg/L

95 % confidence limit: 88-153 mg/L

EC 50 (24 h) calculated: > 100 mg/L

NOEC (48 h): 46 mg/L

EC 0 (48 h): 46 mg/L

EC 100 (48h): > 100 mg/L