5-thiazolylmethanol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 01 - February 03, 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: done under GLP and OECD method

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

and OECD nr. 202 part 1

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

5-thiazolylmethanol

EC Number:

414-780-9

EC Name:

5-thiazolylmethanol

Cas Number:

38585-74-9

Molecular formula:

C4H5NOS

IUPAC Name:

(1,3-thiazol-5-yl)methanol

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

samples (50 ml in duplicate) taken from control and each exposure groups (replicates pooled) at 0 hours and 48 hours and analyzed.

Test solutions

Vehicle:

yes

Details on test solutions:

2.2, 4.6, 10, 22, 46, 100, 220mg/l (nominal) solutions of A-87440.0 in Elendt M7 medium

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Source: IRChA, France

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

Evaluations at 24hr and 48hr

Test conditions

Test temperature:

20°C ± 1°C

pH:

7.3 - 7.5

Dissolved oxygen:

8.6 - 8.7mg/L

Nominal and measured concentrations:

Nominal: 2.2, 4.6, 10, 22, 46, 100, 220mg/L
Measured: 2.1, 4.2, 9.1, 20, 43, 93, 210mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass jars
- Type: loosely covered with aluminium foil to help reduce evaporation.
- fill volume: 200 ml
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

OTHER TEST CONDITIONS
- Photoperiod: 16hr light, 8hr dark

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

110 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 86 - 130 (95% CL)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

44 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 33 - 58 (95% CL)

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

9.1 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

HIghest conc resulting in 0% immobilisation: 9.1mg/l
Lowest conc resulting in 100% immobilisation: 210mg/l

Any other information on results incl. tables

All results expressed in terms of measured concentrations. Measured concentrations ranged from 90 -95% of nominal at 0 hours and 89 -102% of nominal at 48 hours.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48hr EC 50 (immobilisation) value for A-87440.0 with Daphnia Magna is 44mg/l