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EC number: 444-960-2 | CAS number: 39148-16-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 5 Dec 2016 - 4 Jan 2017
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Version / remarks:
- 1984
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): The direct addition method was adopted to prepare the test medium. First, a fixed quantity of the test substance was mixed with the dry test soil, total quantity 10 g, then it was added to the test container and mixed thoroughly with 550 g artificial soil (dry soil), and finally deionised water was used to adjust the soil moisture content to approximately 35% (soil dry weight). See details in Table 1 under any other information on materials and methods incl. tables.
- Controls: negative control without test material
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: earthworm
- Source: Nanjing Earthworm Farm, Pudong Community, Changlu Street, Dachang District, Nanjing City, (batch no.: E20160318E)
- Age at test initiation (mean and range, SD): two months with clitellum
- Weight at test initiation (mean and range, SD): 385-431 mg
ACCLIMATION
- Acclimation period: 277 d, before the test started the test earthworms were cultivated for 24 h in the test soil environment. Prior to use, they were rinsed clean with deionised water and surface water was wiped off with filter paper
- Acclimation conditions (same as test or not): pre-cultivated in pollution-free soil under test conditions - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Test temperature:
- 19.3-20.6 °C
- pH:
- 6.24-6.78
- Moisture:
- 27-35% of soil dry weight
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1 L specimen bottle, plastic film was used to seal the opening of the specimen bottle, and the plastic film was pierced with several small holes for ventilation
- Amount of soil or substrate: 560 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Artificial soil: 1) 10% peat (Sphagnum palustre) (Denmark Pindstrup (Group) C pH value 6.00, no obvious plant residue, finely ground, air dried);
2) 20% kaolin clay (CAS: 1332-58-7, batch no.: 20160118, Sinopharm Group Chemical Reagents Co., Ltd., 30% kaolinite content);
3) 70% quartz sand (CAS: 14808-60-7, batch no.: 20160520, Sinopharm Group Chemical Reagents Co., Ltd., 80% 50-200 μm grain diameter fine sand content)
OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 478-563 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : At 7 d of exposure, the test medium in the culture bottle was gently poured into a stainlesssteel plate, the earthworms were removed, the response of the anterior tail of the earthworm to mechanical stimulus was tested, and symptoms and number of deaths were recorded. If the anterior tail of the earthworm had no obvious response to gentle mechanical stimulus, then it was judged dead. Earthworms that disappeared were also counted among the number of deaths. The test medium was again placed in a culture bottle, and surviving earthworms were placed on the surface of the test medium to continue culturing. At 14 d, the same test was performed again. At the same time as the mortality rate tests on 7 d and 14 d, we observed symptoms of pathology and abnormal behaviour in the earthworms (for example, weakness and stiffness). Bodyweight of the eartworms was measured on days 0 and 14. The pH was measured at days 0 and 14. Test medium soil moisture content was measured at days 0 and 14.
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 100, 500, and 1000 mg/kg (soil dry weight) and blank control
- Results used to determine the conditions for the definitive study: No deaths at any tested concentration after 14 days of exposure - Reference substance (positive control):
- yes
- Remarks:
- 2-chloroacetamide, reference compound test is performed three times each year, to confirm that the test conditions meet the test requirements and there have been no significant changes, test dates of last testing: 2016-11-10 to 2016-11-24
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period: In the pretesting and the limit test processes, no deaths occurred in the control group; at 14 d, there were no earthworm deaths in the 1000 mg/kg (soil dry weight) concentration group
- Other biological observations: At the start of the test, after the earthworms came into contact with the pesticide in soil they quickly burrowed into the soil and were active in the soil during the entire test. - Results with reference substance (positive control):
- - Relevant effect levels: The reference compound’s 7d-LC50 was 35.1 mg/kg and 14d-LC50 was 27.2 mg/kg. The results of this test were valid.
- Reported statistics and error estimates:
- LC50 and 95% confidence interval were calculated using the trimmed Spearman-Karber method (version 1.5, USEPA). Because the test substance had low toxicity to earthworms, the method was only used for calculating the reference compound LC50 and the 95% confidence interval.
- Validity criteria fulfilled:
- yes
- Remarks:
- see Table 3 in 'any other information on results incl. tables'
Reference
Table 1.Average Earthworm Body Weight at Start and End of Exposure Period
Treatment dose (mg/kg (soil dry weight)) |
0 d average body weight (mg) |
14 d average body weight (mg) |
0 |
417 |
386 |
0 |
423 |
375 |
0 |
431 |
385 |
0 |
425 |
377 |
1000 |
385 |
352 |
1000 |
406 |
372 |
1000 |
428 |
381 |
1000 |
411 |
374 |
Minimum value |
385 |
352 |
Maximum value |
431 |
386 |
Table 2.Results of Measurement of Fosetyl-Na Acute Toxicity to Earthworms (Limit test)
Treatment dose (mg/kg (soil dry weight)) |
Earthworm count (each) |
Death count (each) |
|
|
|
7 d |
14 d |
0 |
10 |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
10 |
0 |
0 |
1000 |
10 |
0 |
0 |
1000 |
10 |
0 |
0 |
1000 |
10 |
0 |
0 |
1000 |
10 |
0 |
0 |
Table 3. Validity criteria OECD 207
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The mortality in the controls should not exceed 10 per cent at the end of either test. |
0% |
Yes |
Description of key information
LC50 (14 d) > 1000 mg/kg dw for Eisenia fetida (OECD 207)
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 1 000 mg/kg soil dw
Additional information
A GLP study according to OECD guideline 207 on the acute toxicity to earthworms (Eisenia fetida) using artificial soil was performed with Fosetyl-Sodium. Two months old earthworms with clitellum were exposed to a nominal limit concentration of 1000 mg/kg dry weight and an untreated control at 19.3 - 20.6 °C for 14 days. Appropriate concentrations were obtained by mixing the test substance directly into the dry test substrate. No analytical verification was performed. A test with the reference compound 2-chloroacetamide is performed three times each year, to confirm that the test conditions meet the test requirements and there have been no significant changes. The test dates of the last testing were: 2016-11-10 to 2016-11-24.
After 14 days of exposure, no mortalities were observed in the control or at the test concentration. No significant difference was observed for mean body weight between the control group and the limit concentration. No behavioral alterations or physical abnormalities were observed in the control or in the exposed group after 7 or 14 days exposure to the test substance. Thus, the 14-day LC50 was in excess of the limit concentration of 1000 mg/kg soil dry weight (nominal). The reference compound’s 7d-LC50 was 35.1 mg/kg and 14d-LC50 was 27.2 mg/kg. The results of the test with the reference compound were valid. Since the mortality in the controls did not exceed 10 percent at the end of the test (0%), the test was valid according to the OECD guideline 207 validity criteria.
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