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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017.11.06 - 2017.12.05
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 2011
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Tyrosine
- EC Number:
- 200-460-4
- EC Name:
- Tyrosine
- Cas Number:
- 60-18-4
- Molecular formula:
- C9H11NO3
- IUPAC Name:
- tyrosine
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100, 31.3, 9.77, 3.05, 0.954 mg/L and control.
- Sampling method: taken at 0 hours (initial value) from fresh test solutions and after 24 hours, 48 hours and 72 hours from aged solutions from all test item concentrations and control. At each sampling time point a retention sample was taken.
- Sample storage conditions before analysis: stored deep frozen
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution S1: weighed necessary amount of test item to a volumetric flask and added test medium up to the bench mark, homogenised by shaking. Lower test solutions: dilution of stock solution S1 with test medium
- Evidence of undissolved material: the solution was clear and transparent.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata Hindák
- Strain: SAG 61.81
- Source: commercial supplier
- Age of inoculum (at test initiation):
- Method of cultivation: grown semi-continuously in sterile cultures in the laboratory. Old medium is periodically replaced by fresh mineral solution in order to keep the algae in an exponential growth state.
ACCLIMATION
- Acclimation period: 3-4 days
- Culturing media and conditions: same as test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.7 – 23.1 °C
- pH:
- 7.70 – 8.15
- Nominal and measured concentrations:
- Nominal: 100, 31.3, 9.77, 3.05, 0.954 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks (100 mL) with aluminium caps
- Type: closed
- Fill volume: filled up with ~ 50 mL test solution
- Aeration: CO2 supplied by continuous agitation
- Initial cells density: 0.5 E+04 cells/mL
- Control end cells density: 26.35 E+04 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AAP-Medium
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: The pH was adjusted to 7.5 ± 0.1 with NaOH or HCl, if necessary.
- Photoperiod: Continuously
- Light intensity and quality: fluorescent tubes, 88.8 μEm-2s-1 (mean)
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: fluorescence microplate reader (infinite 200Pro)
- Other: morphological appearance: observed microscopically at the end of the test; pH: Measured at t = 0 h and t = 72 h; temperature: measured continuously and recorded at hours 0, 24, 48 and 72; light intensity: measured once a year and was confirmed for one representative position at test start.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 63.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 63.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 63.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Inhibition: After 72 h at termination of the test no statistically significant inhibition of growth rate or yield was observed for test item concentrations up to and including 100 mg/L. The inhibition of growth rate peaked in 6.6 % and the inhibition of yield peaked in 23.8 % at a nominal test item concentration of 31.3 mg/L.
- Cell morphology: normal for the control and all test item concentrations. At 31.3 and 100 mg/L bacteria were observed in the solutions.
- Analytical results: The measured initial concentration of active ingredient was 81 % of nominal at 100 mg/L. A degradation of the active ingredient was observed during the test with 29 % of nominal measured at test end. The measured initial concentration of active ingredient in the replicates containing the 100 mg/L test solution without algae was 74 % of nominal at 100 mg/L. A degradation of the active ingredient was observed during the test with 46 % of nominal measured at test end. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 1.23 mg/L for growth rate; 0.685 mg/L for yield; 0.639 mg/L for biomass intergral - Reported statistics and error estimates:
- - LOEC and NOEC: Dunnetts-t-test (left-sided, p<0.05) for growth rate and for yield
- ECx: Due to an inhibition below 50% the database was weak for probit analysis which hence was not performed
Any other information on results incl. tables
Toxicological endpoints for the test item:
| Test item [mg/L] | |
nominal | Actual (geometric mean) | |
ErC10 (Growth rate) | >100 | >63.2 |
95% confidential limits | n.c | n.c |
ErC20 | >100 | >63.2 |
95% confidential limits | n.c. | n.c. |
ErC50 | >100 | >63.2 |
95% confidential limits | n.c. | n.c. |
EyC10 (Yield) | n.d. | n.d. |
95% confidential limits | n.c. | n.c. |
EyC20 | n.d. | n.d. |
95% confidential limits | n.c | n.c |
EyC50 | >100 | >63.2 |
95% confidential limits | n.c. | n.c. |
NOEC | 100 | 63.2 |
LOEC | - | - |
n.d.: Not determined
n.c : Not caculated
Individual cell numbers:
Conc. [mg/L] | Cell numbers [1E+04/mL] | Yield | |||
0h | 24h | 48h | 72h | 0h–72h | |
Control | 0.63 | 1.29 | 5.63 | 25.52 | 24.89 |
0.51 | 1.32 | 6.05 | 28.78 | 28.27 | |
0.52 | 1.17 | 5.02 | 17.18 | 16.66 | |
0.54 | 1.63 | 6.07 | 26.49 | 25.95 | |
0.54 | 1.77 | 7.57 | 32.39 | 31.85 | |
0.51 | 1.68 | 6.73 | 27.75 | 27.24 | |
Mean | 0.541) | 1.48 | 6.18 | 26.35 | 25.81 |
0.954 | 0.54 | 1.01 | 5.77 | 25.48 | 24.94 |
0.54 | 1.65 | 6.24 | 25.24 | 24.70 | |
0.54 | 1.75 | 6.80 | 25.53 | 24.99 | |
Mean | 0.54 | 1.47 | 6.27 | 25.42 | 24.88 |
3.05 | 0.54 | 1.18 | 5.74 | 20.00 | 19.46 |
0.54 | 1.74 | 6.87 | 29.90 | 29.36 | |
0.54 | 1.62 | 5.96 | 22.65 | 22.11 | |
Mean | 0.54 | 1.51 | 6.19 | 24.18 | 23.64 |
9.77 | 0.54 | 1.43 | 5.44 | 21.67 | 21.13 |
0.54 | 1.61 | 5.99 | 25.25 | 24.71 | |
0.54 | 1.49 | 6.09 | 25.38 | 24.84 | |
Mean | 0.54 | 1.51 | 5.84 | 24.10 | 23.56 |
31.3 | 0.54 | 1.13 | 4.03 | 17.55 | 17.01 |
0.54 | 1.46 | 5.66 | 18.76 | 18.22 | |
0.54 | 1.23 | 6.37 | 24.32 | 23.78 | |
Mean | 0.54 | 1.27 | 5.35 | 20.21 | 19.67 |
100 | 0.54 | 1.88 | 6.32 | 28.60 | 28.06 |
0.54 | 1.76 | 7.00 | 28.50 | 27.96 | |
0.54 | 1.59 | 7.29 | 31.67 | 31.13 | |
Mean | 0.54 | 1.74 | 6.87 | 29.59 | 29.05 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No statistically significant inhibitory effects on any parameter (growth rate, yield) were observed at any of the test item concentrations, including the highest test item concentration of 100 mg/L (nominal) and 63.2 mg/L (actual) at test end. Thus the overall LOEC was not determinable and the overall NOEC was observed to be at 100 mg/L (nominal) and 63.2 mg/L (actual).
The EC10-, EC20- and EC50-value for growth rate and the EC50-value for yield were considered to be > 100 mg/L (nominal) and > 63.2 mg/L (actual). - Executive summary:
O-phospho-L-tyrosine dissociates in aqueous solutions. As according to REACH regulation only publicly available data has to be provided with the Registration dossier for on-site isolated intermediates in a tonnage band of < 1,000 t/a. Thus, information on the paren compound L-tyrosin is considered relevant and will be used for the registration dossier of O-phospho-L-tyrosine.
The effects of test item on the growth of the single cell green alga Pseudokirchneriella subcapitata were determined according to OECD 201.
A static test with nominal test item concentrations of 100, 31.3, 9.77, 3.05 and 0.954 mg/L and control was performed. Six replicates were employed for the control and three for each test item concentration. One replicate with 100 mg/L test item concentration without algae was run in parallel which was used for the analytical sampling. The test was performed in 100 mL Erlenmeyer flasks each containing ~ 50 mL test solution.
Toxicological endpoints were evaluated using the nominal and actual concentrations (based on the geometric mean) of the test item.
No statistically significant inhibitory effects on any parameter (growth rate, yield) were observed at any of the test item concentrations, including the highest test item concentration of 100 mg/L (nominal) and 63.2 mg/L (actual) at test end. Thus the overall LOEC was not determinable and the overall NOEC was observed to be at 100 mg/L (nominal) and 63.2 mg/L (actual).
The EC10-, EC20- and EC50-value for growth rate and the EC50-value for yield were considered to be > 100 mg/L (nominal) and > 63.2 mg/L (actual). Due to an inhibition of yield below 50 % and a missing concentration response relation no reliable values were calculable and the EC10- and EC20-value for yield were not determined.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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