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EC number: 416-530-4 | CAS number: 178949-82-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Soil samples were incubated with the test substance and basal and substrate-induced respiration, enzyme activity and potentially mineralizable nitrogen were measured; community level physiological profiles were also determined.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Vapour pressure: 0.00017 Pa at 25°C
- Henry's law constant (for volatile substances): no data
- Water solubility (under test conditions): ca. 150 mg/L at 25°C and pH 7
- Solubility in organic solvents:
- log Pow: <-1.4
- pKa: no data
- Base or acid catalysis of test material: no data
- log Koc: 1.778
- UV absorption: no data
- Stability of test material at room temperature: stable
- pH dependance on stability: no data - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 1.0 g/kg dw soil
- Sampling method: using MOM-type Rhizons to extract the soil solution free of microbial and colloid contamination.
- Sample storage conditions before analysis: no data
Rate of degradation of EDDS assessed over 3 weeks - Vehicle:
- not specified
- Details on preparation and application of test substrate:
- no data
- Test organisms (inoculum):
- soil
- Total exposure duration:
- 3 d
- Remarks:
- for respiration studies
- Test temperature:
- no data
- Moisture:
- no data for enzyme studies
60% for respiration studies - Details on test conditions:
- TEST SYSTEM
- Testing facility: greenhouse
- Test container (type, material, size): 350 cm3 plastic pots
- Amount of soil: 250 g dry weight
- No. of replicates per concentration: 4
- No. of replicates per control: no 4
- No. of replicates per vehicle control: no data
SOIL INCUBATION
- Method: series of individual subsamples
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographical reference of sampling site (latitude, longitude): 43.37 N; 2.82 W (Larrauri, Spain)
- History of site: no data
- Vegetation cover: natural grassland
- Treatments with pesticides or fertilizers: no data
- Accidental contamination: no data
- Depth of sampling: 20 cm
- Soil texture
- % sand: 15.7 (coarse); 36.3 (fine)
- % silt: 24.4
- % clay: 23.7
- Soil taxonomic classification: no data
- Soil classification system: no data
- pH (in water): 6.9
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight): no data
- Maximum water holding capacity (in % dry weigth): 35.5 (at 33 kPa)
- Cation exchange capacity (mmol/kg): no data
- Pretreatment of soil: air-dried for 24 h before sieving through a 2 mm sieve
- Storage (condition, duration): 4 degrees C
- Initial microbial biomass as % of total organic C: no data
-C/N: 13
DETAILS OF PREINCUBATION OF SOIL (if any): no data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : dehydrogenase, acid phosphatase, ¿-glucosidase, and arylsulphatase; mineralizable nitrogen; substrate-induced respiration; metabolic quotients (qCO2; calculated as the ratio between the values of basal respiration and substrate-induced respiration); community level physiological profiles (assessed using Biolog EcoPlates)
VEHICLE CONTROL PERFORMED: no data
RANGE-FINDING STUDY
- Test concentrations: no data - Nominal and measured concentrations:
- 1.0 g/kg dw soil
- Reference substance (positive control):
- no
- Key result
- Duration:
- 3 d
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1 g/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- respiration rate
- Details on results:
- The addition of EDDS to the soil sample did not result in any statistically significant differences in the activity of the enzymes dehydrogenase, acid phosphatase, glucosidase and arylsulphatase (see Table 1). The potentially mineralizable nitrogen was unchanged compared to control samples, indicating that EDDS did not affect the biological activity of the soil (Table 1).
Although the test substance caused a small, but significant inhibition of soil basal respiration, it did not affect substrate-induced respiration. Due to the decrease in soil basal respiration, a significant decrease in the qCO2 was evident in the presence of the test substance (Figure 1; attached).
In the test for functional diversity of the soil microflora, EDDS caused a significant increase in species richness and Shannon's diversity (total number of species present), but showed no effects on Shannon's evenness or average well colour development (AWCD) (see Table 2). - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Statistically significant differences of P<0.05 or lower were reported
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a study using several endpoints to measure toxicity to soil micro-organisms, EDDS at 1.0 g/kg dw soil for 3 days (considered the study NOEC) did not affect substrate-induced respiration (used as an indicator of potential biomass activity) or the activity of four enzymes (considered important for the breakdown of organic matter), although it did cause a significant inhibition of soil basal respiration.
- Executive summary:
Ethylenediamine disuccinate (EDDS) was studied for its effects on soil micro-organisms (microbial biomass, activity and diversity and community structure), using soil samples from a natural grassland in Larrauri, Northern Spain.
After the addition of 1.0 g EDDS/kg dw soil, basal respiration (as an indicator of overall microbial activity) was measured and compared to substrate-induced respiration (as a measure of potentially active biomass) after the addition of glucose. Metabolic quotient (qCO2) was then calculated as the ratio of these two measurements to determine the effect of the test substance on the soil ecosystem. The activity of dehydrogenase, acid phosphatase, glucosidase, and arylsulphatase were analyzed as representatives of four different classes of enzymes important for the breakdown of organic matter. Potentially mineralizable nitrogen was also measured as an indicator of the biological soil activity. Finally, substrate profiles were assessed to determine the functional diversity of the soil microflora.
Basal respiration was reduced slightly (by about 10%), whilst substrate-induced respiration was unaffected by the addition of the test substance. The qCO2 was significantly reduced compared to the control value, indicating development of the ecosystem. There were no significant changes in enzyme activities or potentially mineralizable nitrogen when compared to the controls. Functional diversity was actually increased by the presence of EDDS.
In conclusion, 1 g EDDS/kg dw soil had no significant detrimental effect on the soil microflora; this value is considered the study 3-d NOEC.
[Information on EDDS is considered relevant to use for understanding the potential effects of trisodium EDDS on microbial toxicity, and is acceptable for using as read-across information.]
Reference
Table 1 - effect of EDDS on soil enzyme activities and potentially mineralizable nitrogen
|
mg iodo-nitrotetrazolium formazane/kg dry wt soil/h
Dehydrogenase |
mg p-nitrophenol/kg dry wt soil/h
Arylsulphatase Glucosidase Acid phosphatase
|
mg N-NH3/kg dry wt soil
Potentially mineralizable N |
||
Control |
448.3 ± 20.1 |
202.3 ± 16.8 |
227.8 ± 6.0 |
1070.0 ± 52.6 |
8.1 ± 0.3 |
EDDS |
420.1 ± 56.8 |
204.5 ± 11.7 |
242.1 ± 3.5 |
1168.8 ± 64.1 |
9.0 ± 1.2 |
Table 2. Effect of EDDS on the AWCD and diversity indexes calculated from Biolog EcoPlate; absorbance data at an incubation time of 48 h
AWCD | S | H | J | |
Control | 0.49±0.08 | 15.67±1.20 | 2.64±0.0 | 0.67±0.00 |
EDDS | 0.62±0.08 | 19.67±1.20 | 2.85±0.0 | 0.66±0.00 |
AWCD; average well colour development; S; richness; H; Shannon's diversity index; J: Shannon's evenness index
Description of key information
In a study using several endpoints to measure toxicity to soil micro-organisms, EDDS at 1.0 g/kg dw soil for 3 days (considered the study NOEC) did not affect substrate-induced respiration (used as an indicator of potential biomass activity) or the activity of four enzymes (considered important for the breakdown of organic matter), although it did cause a significant inhibition of soil basal respiration
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 1 000 mg/kg soil dw
Additional information
Ethylenediamine disuccinate (EDDS) was studied for its effects on soil micro-organisms (microbial biomass, activity and diversity and community structure), using soil samples from a natural grassland in Larrauri, Northern Spain.
After the addition of 1.0 g EDDS/kg dw soil, basal respiration (as an indicator of overall microbial activity) was measured and compared to substrate-induced respiration (as a measure of potentially active biomass) after the addition of glucose. Metabolic quotient (qCO2) was then calculated as the ratio of these two measurements to determine the effect of the test substance on the soil ecosystem. The activity of dehydrogenase, acid phosphatase, glucosidase, and arylsulphatase were analyzed as representatives of four different classes of enzymes important for the breakdown of organic matter. Potentially mineralizable nitrogen was also measured as an indicator of the biological soil activity. Finally, substrate profiles were assessed to determine the functional diversity of the soil microflora.
Basal respiration was reduced slightly (by about 10%), whilst substrate-induced respiration was unaffected by the addition of the test substance. The qCO2 was significantly reduced compared to the control value, indicating development of the ecosystem. There were no significant changes in enzyme activities or potentially mineralizable nitrogen when compared to the controls. Functional diversity was actually increased by the presence of EDDS. In conclusion, 1 g EDDS/kg dw soil had no significant detrimental effect on the soil microflora; this value is considered the study 3 -d NOEC (Epelde et al. 2008).
[Information on EDDS is considered relevant to use for understanding the potential effects of trisodium EDDS on microbial toxicity, and is acceptable for using as read-across information.]
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