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EC number: 416-530-4 | CAS number: 178949-82-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water and sediment: simulation tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: sediment simulation testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 6 March to 7 April 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Directive 79/831/EEC (1987). Part C : Methods for the Determination of Ecotoxicity: Blodegradation Activated Sludge Simulation Tests.
- Deviations:
- no
- GLP compliance:
- yes
- Radiolabelling:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: the inoculum used is activated sludge obtained from the aerators of the treatment plant of Bochott dealing predominantly with domestic sewage
- Concentration of sludge: 2.5 g/L
- Initial cell/biomass concentration: no data - Duration of test (contact time):
- ca. 31 d
- Initial conc.:
- ca. 20 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Volume of test solution/treatment: trisodium EDDS was added in such a way that an influent concentration of 20 mg C/L was obtained.
- Composition of medium: water containing inoculum at about 2.5 g/L
- Additional substrate: no data
- Solubilising agent (type and concentration if used): water
- Test temperature: 18.1-21.7oC
- pH: 6.8-8.4
- pH adjusted: no
- CEC (meq/100 g): no data
- Aeration of dilution water: no
- Suspended solids concentration: 16.4 g/L
- Continuous darkness: no data
- Any indication of the test material adsorbing to the walls of the test apparatus: no data
TEST SYSTEM
- Culturing apparatus: the equipment consists of a storage vessel for the test substance stock solution, a storage vessel for the sewage influent, a dosing pump, an aeration vessel, a separator, an air-lift pump to recycle the activated sludge, and a vessel for collecting the treated effluent. The test material and the sewage are pumped separately to the aerator by means of two peristaltic pumps.
- Number of culture flasks/concentration: no data
- Method used to create aerobic conditions: air is blown Into the aeration vessel by a sintered aeration plate at the apex of the cone.
- Method used to create anaerobic conditions: not required
- Measuring equipment: no data
SAMPLING
- Sampling frequency:
Nutrient solution: sample for COD 2 times a week.
Effluent: samples for DOC analysis were taken 5 times a week. From day 14, additional 250 mL samples were taken 3 times a week for the sponsor to perform specific analysis if needed.
Mixed Liquor: 2 times a week.
- Sampling method: COD analysis of the nutrient solution was performed within 24 h after samplng. Effluent samples were analysed for DOC either immediately or stored in a refrigerator to be analysed the next day. The additional 250 mL samples were preserved with 3% formaldehyde 35% and stored In a freezer. The determination of suspended solids In the mixed Iiquor was performed immediately after sampling using Whatmann GF/C glass microflbre filters.
- Sterility check if applicable: no data
- Sample storage before analysis: samples were preserved with 3% formaldehyde 35% and stored In a freezer
STATISTICAL METHODS:
Mean, standard deviation and tolerance limits at 95% probablity level was performed on DOC removal results. - Compartment:
- other: water / sediment, material (mass) balance
- Remarks on result:
- other: no data
- % Degr.:
- 73.2
- Parameter:
- DOC removal
- Sampling time:
- 1 d
- % Degr.:
- 6.7
- Parameter:
- DOC removal
- Sampling time:
- 4 d
- % Degr.:
- 87.9
- Parameter:
- DOC removal
- Sampling time:
- 7 d
- % Degr.:
- 93.5
- Parameter:
- DOC removal
- Sampling time:
- 11 d
- % Degr.:
- 97.4
- Parameter:
- DOC removal
- Sampling time:
- 14 d
- % Degr.:
- 99.4
- Parameter:
- DOC removal
- Sampling time:
- 18 d
- % Degr.:
- 99.2
- Parameter:
- DOC removal
- Sampling time:
- 21 d
- % Degr.:
- 59.1
- Parameter:
- DOC removal
- Sampling time:
- 25 d
- % Degr.:
- 90.6
- Parameter:
- DOC removal
- Sampling time:
- 28 d
- % Degr.:
- 90.6
- Parameter:
- DOC removal
- Sampling time:
- 30 d
- Transformation products:
- not measured
- Evaporation of parent compound:
- no
- Volatile metabolites:
- no
- Residues:
- no
- Details on results:
- TEST CONDITIONS
- Aerobicity, moisture, temperature and other experimental conditions maintained throughout the study: yes
- Anomalies or problems encountered (if yes): COD values of the nutrient solution were occasionally just outside the recommended range 350-400 mg/L.
MAJOR TRANSFORMATION PRODUCTS
- Range of maximum concentrations in % of the applied amount and day(s) of incubation when observed: no data
- Range of maximum concentrations in % of the applied amount at end of study period: no data
MINOR TRANSFORMATION PRODUCTS
- Range of maximum concentrations in % of the applied amount and day(s) of incubation when observed: no data
- Range of maximum concentrations in % of the applied amount at end of study period: no data
TOTAL UNIDENTIFIED RADIOACTIVITY (RANGE) OF APPLIED AMOUNT: no data
EXTRACTABLE RESIDUES
- % of applied amount at day 0: no data
- % of applied amount at end of study period: no data
NON-EXTRACTABLE RESIDUES
- % of applied amount at day 0: no data
- % of applied amount at end of study period: no data
MINERALISATION
- % of applied radioactivity present as CO2 at end of study: no data
VOLATILIZATION
- % of the applied radioactivity present as volatile organics at end of study: no data
STERILE TREATMENTS (if used)
- Transformation of the parent compound: no data
- Formation of transformation products: no data
- Formation of extractable and non-extractable residues: no data
- Volatilization: no data
RESULTS OF SUPPLEMENTARY EXPERIMENT (if any): no data - Validity criteria fulfilled:
- yes
- Conclusions:
- The biodegradation of trisodium EDDS was monitored in an activated sludge simulation test (DOC removal) conducted according to OECD Guideline 303 A and in compliance with GLP. The mean DOC removal value, based on 17 measurements taken between days 8-30 (with one result excluded) was 95.6% (SD 3.3%). Therefore, trisodium EDDS was shown to be readily biodegradable.
- Executive summary:
In a GLP study conducted according to OECD Guideline 303 A, the biodegradation of trisodium EDDS was assessed in an activated sludge simulation test (DOC removal).
Raw, predominantly domestic, sewage from a municipal treatment facility was used (occasionally enriched with synthetic sewage) and DOC measured for about 31 days in a test unit (with test substance added at 20 mg C/L) and a control unit (receiving only the sewage inoculum). DOC concentrations were measured in both units and the difference was taken to reflect undegraded test material. The mean DOC removal value, based on 17 measurements taken between days 8-30 (with one result excluded) was 95.6% (SD 3.3%). Therefore, trisodium EDDS was shown to be readily biodegradable in this system.
- Endpoint:
- biodegradation in water: simulation testing on ultimate degradation in surface water
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- the study does not need to be conducted because the substance is readily biodegradable
Referenceopen allclose all
Table.1: Measured temperature, pH and DO (Dissolved oxygen) concentration in the mixed liquor
Date |
Day of test |
Control Unit |
Test unit |
||||
T (oC) | pH | DO (mg/L) | T (oC) | pH | DO (mg/L) | ||
08/03/93 | 0 | 18.4 | 6.92 | 8.3 | 18.1 | 6.98 | 7.9 |
11/03/93 | 3 | 20.3 | 6.83 | 8.1 | 20.2 | 7.30 | 8.1 |
15/03/93 | 7 | 19.8 | 7.59 | 6.8 | 19.8 | 7.82 | 7.4 |
18/03/93 | 10 | 21.0 | 8.07 | 7.3 | 20.9 | 8.09 | 8.0 |
22/03/93 | 14 | 19.3 | 8.29 | 8.7 | 19.1 | 8.43 | 8.1 |
25/03/93 | 17 | 21.0 | 8.17 | 8.0 | 21.0 | 8.31 | 7.7 |
29/03/93 | 21 | 21.3 | 8.17 | 7.6 | 21.3 | 7.86 | 7.6 |
01/04/93 | 24 | 21.7 | 7.38 | 7.7 | 21.7 | 7.36 | 7.4 |
05/04/93 | 28 | 21.1 | 6.40 | 7.9 | 21.3 | 7.15 | 7.6 |
Table 2: COD values of the sewage
Date | Day of the test | COD (mg/L) |
08-03-1993 | 0 | 310* |
11-03-1993 | 3 | 295* |
15-03-1993 | 7 | 388 |
19-03-1993 | 11 | 345 |
22-03-1993 | 14 | 360 |
24-03-1993 | 16 | 444* |
26-03-1993 | 18 | 392 |
29-03-1993 | 21 | 348 |
01-04-1993 | 24 | 404 |
06-04-1993 | 29 | 368 |
* enrichment with synthetic sewage
Description of key information
The biodegradation of trisodium EDDS was assessed in an activated sludge simulation test (DOC removal) conducted according to OECD Guideline 303 A and in compliance with GLP.
The mean DOC removal value, based on 17 measurements taken between days 8-30 (with one result excluded) was 95.6% (SD 3.3%). Therefore, trisodium EDDS was shown to be readily biodegradable.
Key value for chemical safety assessment
Additional information
In a GLP study conducted according to OECD Guideline 303 A, the biodegradation of trisodium EDDS was assessed in an activated sludge simulation test (DOC removal).
Raw, predominantly domestic sewage from a municipal treatment facility was used (occasionally enriched with synthetic sewage) and DOC measured for about 31 days in a test unit (with test substance added at 20 mg C/L) and a control unit (receiving only the sewage inoculum). DOC concentrations were measured in both units and the difference was taken to reflect undegraded test material. The mean DOC removal value, based on 17 measurements taken between days 8-30 (with one result excluded) was 95.6% (SD 3.3%). Therefore, trisodium EDDS was shown to be readily biodegradable in this system (Geukens, 1993b).
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