Reaction product of castor oil with glycerol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

31 Jan - 28 Feb 2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

Version 1992

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: US EPA OCSPP 835.3110

Version / remarks:

Paragraph (q)

GLP compliance:

yes (incl. QA statement)

Remarks:

Date of certificate: 19.11.2018 (The Department of Health of the Government of the United Kingdom)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction product of castor oil with glycerol

EC Number:

949-117-7

Molecular formula:

not applicable, substance is UVCB

IUPAC Name:

Reaction product of castor oil with glycerol

Test material form:

liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, procedure): final effluent stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK; sample of effluent was filtered through coarse filter paper (first approximate 200 mL discarded)
- Storage conditions: maintained on aeration in a temperature controlled room at temperatures of between 20 and 21 °C
- Concentration of sludge: inoculum at a rate of 1% v/v in test vessels
- Initial cell/biomass concentration: 5 mL inoculum per 500 ml test volume
- Water filtered: yes
- Type and size of filter used, if any: coarse filter paper

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: mineral medium according to OECD guideline
- Test temperature: 20 - 21 °C
- pH: 7.4 - 8.0
- pH adjusted: no
- Continuous darkness: yes
- Other: test was conducted in diffuse light

TEST SYSTEM
- Culturing apparatus: 500 ml amber glass bottles sealed with a sensor head/CO2 trap immersed in a temperature controlled water bath
- Number of culture flasks/concentration: 3 replicates for test item
- Method used to create aerobic conditions: stirring of test vessels with a magnetically coupled stirrer
- Measuring equipment: CES Multi-Channel Aerobic Respirometer
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: CO2 is absorbed into an ethanolamine solution (50% v/v) causing a net reduction in gas pressure within the sample flask, pressure reduction triggers the electrolytic process, generating oxygen and restoring the pressure in the sample flask. The magnitude of the electrolyzing current and the duration of the current is proportional to the amount of oxygen supplied to the micro-organisms


SAMPLING
- Sampling frequency: daily
- Sampling method: data generated from the respirometer’s own battery backed memory was collected on the hard disk drive of a non-dedicated computer
- Other: on day 28 two inoculum controls, one procedure control, two test item and one toxicity control vessel were sampled for pH analysis

CONTROL AND BLANK SYSTEM
- Inoculum blank: 3 replicates
- Abiotic sterile control: none
- Toxicity control: 2 replicates
- Other: 2 replicates for positive control

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

60

Sampling time:

28 d

Remarks on result:

other: Mean of two replicates

BOD5 / COD results

Results with reference substance:

Aniline attained 69% biodegradation after 14 days and 74% biodegradation after 28 days

Any other information on results incl. tables

Table 1. Percentage Biodegradation Values

Day	Biodegradation (%)
	Procedure Control	Test Item			Toxicity Control
		R1	R2	Mean
0	0	0	0	0	0
1	0	0	0	0	1
2	0	16	16	16	6
3	0	25	25	25	8
4	2	29	29	29	18
5	16	32	31	32	28
6	24	33	32	33	41
7	36	36	34	35	49
8	57	37	35	36	51
9	63	39	37	38	51
10	64	40	38	39	52
11	66	42	40	41	53
12	67	44	43	44	54
13	68	46	46	46	54
14	69	47	48	48	55
15	70	49	49	49	55
16	70	51	50	51	56
17	71	53	50	52	57
18	72	54	51	53	57
19	72	55	51	53	58
20	72	56	52	54	59
21	73	56	52	54	60
22	73	57	53	55	61
23	73	58	53	56	62
24	74	59	54	57	63
25	74	60	54	57	64
26	74	61	55	58	65
27	74	62	56	59	66
28	74	63	57	60	66

 

 

 

 

 

 

 

Table 2: Validity criteria for OECD 301F

Criterion from the guideline	Outcome	Validity criterion fulfilled
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%.	< 20%	Yes
Percentage degradation of the reference compound reached the pass level by day 14 (≥ 60%).	69 %	Yes
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d.	55%	Yes
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/L and should not be greater than 60 mg/L in 28 days.	25.53 mg O2/L at 28 d	Yes

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

See Table 2 in "any other information on results incl. tables"

Interpretation of results:

readily biodegradable, but failing 10-day window

Remarks:

As the test item was a UVCB, the 10-day window criteria does not apply and therefore the test item can be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301F.