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EC number: 212-506-0 | CAS number: 822-89-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-06-08 to 1994-06-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: EC Directive 87/302 'Biodegradation - activated Sludge Respiration Inhibition test'
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Hydroxyl-2-pyridone
- EC Number:
- 212-506-0
- EC Name:
- Hydroxyl-2-pyridone
- Cas Number:
- 822-89-9
- Molecular formula:
- C5H5NO2
- IUPAC Name:
- 1-hydroxy-1,2-dihydropyridin-2-one
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Name of test material (as cited in study reports): Oxy PYRION
- Physical state: solid (powder)
- Appearance: white speckled gold powder
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: batch number 93100020
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at ambient temperature in the original container
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test concentrations of Oxypyrion were prepared from aqueous stock solutions (1 and 2 g/L) whose pH had been adjusted to approximate neutrality with sodium hydroxide (5N). Test concentrations up to and including 560 mg/L were prepared from the 1 g/L stock and at 1000 mg/L from the 2 g/L stock.
- Differential loading: Two tests were conducted; the first with nominal test concentrations of 1, 10 and 100 mg/L, and the seconde with 56, 100, 320, 560 and 1000 mg/L.
- Controls: yes
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: A sample of activated sludge was obtained from Oakley sewage treatment works, a sewage plant treating predominantly domestic waste
- Preparation of inoculum for exposure:The concentration of suspended solids in a homogenised sample was determined on the day of collection and immediately before the start of the test. Aliquots (25 mL) of the activated sludge were filtered through dried and preweighed Whatman's GFC filter papers which were then dried again at 105°C for at least one hour, allowed to cool in a dessicator and reweighed. The mixed liquor suspended solids (MLSS) content of the activated sludge was then calculated and adjusted where necessary to fall in the range 2 to 4 g/L by the addition of dechlorianted tap water. Synthetic sewage (50 mL/L) was added when necessary and the mixture aerated overnight.
- Initial biomass concentration: 1.6 g SS/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- After 3h of exposure, a well-mixed sample of each mixture was transferred to a biochemical oxygen demand (BOD) bottle and its rate of oxygen consumption was measured over a period of ~10 minutes using a Yellow Springs Instruments (YSI) dissolved oxygen meter.
Test conditions
- Test temperature:
- - Preliminary test:
* initial: 19.1 - 20.5
* Final: 18.4 - 19.2
- Final test:
* Initial: 19.8 - 20.5
* Final: 19.6 - 20.7 - pH:
- - Preliminary test:
* initial: 7.8-8.1
* Final: 7.9-8.6
- Final test:
* Initial: 7.5-7.8
* Final: 8.3-8.5 - Dissolved oxygen:
- not specified
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Loading rates for the test substance:
- Preliminary test: 1, 10 and 100 mg/L
- Final test: 56, 100, 320, 560 and 1000 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: no data
- Material, size, headspace, fill volume: no data
- Aeration: the mixture was aerated overnight
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Sludge concentration (weight of dry solids per volume): 2 to 4 g SS/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ultrapure water
OTHER TEST CONDITIONS
- Adjustment of pH: pH had been adjusted to approximate neutrality with sodium hydroxide (5N)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Range finding study yes
- Test concentrations: 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 320.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: 95% CL: 261.5 - 391.4 mg/L
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 115 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Blank controls oxygen uptake rate: The specific respiration rates of the control cultures established at the end of the 2 tests series were 99% and 108% of the rate of those established at the start.
- At 1000 mg/L 77% inhibition was observed - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: The EC50 for 3,5-DCP in the preliminary test was calculated to be 7.9 mg/L and in the final test 9.1 mg/L. This is within the recommended range of 2-25 mg/L confirming suitability of the activated sludge used.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- An activated sludge inhibition test (according OECD guideline 209) with activated sludge from a predominantly domestic sewage treatment plant resulted in a 3-h EC50 of 320.8 mg Oxypyrion/L. The results of the study can be considered reliable without restriction.
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