Methyltrioctylammonium chloride

Endpoint summary

Description of key information

Acute Oral Toxicity: 

In Acute oral toxicity,LD50 value for target substance Methyltricaprylyl Ammonium Chloride (5137-55-3) was considered to be 223 mg/kg bw in rats and 280 mg/kg bw in mice,and for differentstudies available on the structurally similar read across substancewas considered in the range of 250-300 mg/kg bw in rats. All these studies concluded that the LD50 value is between 50-300 mg/kg bw.Thus, comparing this value with the criteria of CLP regulation, Methyltricaprylyl Ammonium Chloride (5137-55-3) can be classified as category III of acute oral toxicity.

Acute Inhalation Toxicity: 

Methyltricaprylyl Ammonium Chloride (5137-55-3) has very low vapor pressure (2.5E-010 Pa.).So the potential for the generation of inhalable vapours is very low. Also the normal conditions of use of this substance will not result in aerosols, particles or droplets of an inhalable size, so exposure to humans via the inhalatory route will be highly unlikely and therefore the acute inhalation toxicity end point was considered for waiver.

Acute Dermal Toxicity:

The acute dermal toxicity dose (LD50) for Methyltricaprylyl Ammonium Chloride (5137-55-3) was based on data available for the structurally similar read across chemicals. The LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, Methyltricaprylyl Ammonium Chloride (5137-55-3) cannot be classified for acute dermal toxicity. 

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Reference

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

data is from authoritative database

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Acute Oral toxicity test was carried out to study the effects of Methyltricaprylyl Ammonium Chloride (5137-55-3) on rats.

GLP compliance:

not specified

Test type:

other: no data available

Limit test:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report):methyl(trioctyl)azanium chloride
- Molecular formula :C25H54ClN
- Molecular weight :404.162 g/mol
- Substance type:organic
- Physical state:Pale yellow viscous liquid

Species:

rat

Strain:

not specified

Sex:

male

Details on test animals or test system and environmental conditions:

No data available

Route of administration:

oral: unspecified

Vehicle:

peanut oil

Details on oral exposure:

Details on exposure
VEHICLE
- Justification for choice of vehicle: test substance was soluble in Peanut oil.

Doses:

223 mg/kg bw

No. of animals per sex per dose:

223 mg/kg bw: 6 male rats

Control animals:

not specified

Details on study design:

No data available

Statistics:

No data available

Preliminary study:

No data available

Sex:

male

Dose descriptor:

LD50

Effect level:

223 mg/kg bw

Based on:

test mat.

95% CL:

136 - 366

Remarks on result:

other: 50% mortality was observed at dose 223 mg/kg bw

Mortality:

50% mortality was observed at dose 223 mg/kg bw

Clinical signs:

other: Dominant Clinical signs at lethal dosages like respiratory depression, gastrointestinal hypermotility,diarrhea, labored breathing and discharge from eye were observed.

Gross pathology:

No data available

Other findings:

No data available

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

The lethal concentration (LD50) value for acute oral toxicity test was considered to be 223 mg/kg (95% confidence limits:136-366 mg/kg),when 6 male rats were treated with Methyltricaprylyl Ammonium Chloride (5137-55-3) orally.

Executive summary:

Acute oral toxicity study was done in 6 male rats using test material Methyltricaprylyl Ammonium Chloride (5137-55-3).Peanut oil was used as vehicle.50% Mortality was observed at dose 223 mg/kg bw.Dominant Clinical signs at lethal dosages like respiratory depression, gastrointestinal hypermotility,diarrhea, labored breathing and discharge from eye were observed.Hence,LD50 value was considered to be223 mg/kg bw(95% confidence limits:136-366 mg/kg),when rats were treated with Methyltricaprylyl Ammonium Chloride (5137-55-3)orally.

Endpoint conclusion

Endpoint conclusion:

adverse effect observed

Dose descriptor:

LD50

Value:

223 mg/kg bw

Quality of whole database:

Data is Klimisch 2 and from authoritative database

Acute toxicity: via inhalation route

Link to relevant study records

Reference

Endpoint:

acute toxicity: inhalation

Data waiving:

other justification

Justification for data waiving:

other:

Endpoint conclusion

Quality of whole database:

Waiver

Acute toxicity: via dermal route

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Dose descriptor:

LD50

Value:

6 818.27 mg/kg bw

Quality of whole database:

Data is Klimisch 2 and from study report

Additional information

Acute Oral Toxicity: 

In different studies, Methyltricaprylyl Ammonium Chloride (5137-55-3) has been investigated for acute oral toxicity to a greater or lesser extent. Often the studies are based on in vivo experiments in rodents, i.e. most commonly in rats for Methyltricaprylyl Ammonium Chloride (5137-55-3) along with the study available on the structurally similar read across substance. The studies are summarized as below –

The experimental study mentioned in authoritative database (2018) and secondary source (1992,2018) for the target chemicalMethyltricaprylyl Ammonium Chloride (5137-55-3)was designed and conducted for acute oral toxicity. Acute oral toxicity study was done in 6 male rats using test material Methyltricaprylyl Ammonium Chloride (5137-55-3).Peanut oil was used as vehicle.50% Mortality was observed at dose 223 mg/kg bw.Dominant Clinical signs at lethal dosages like respiratory depression, gastrointestinal hypermotility,diarrhea, labored breathing and discharge from eye were observed.Hence,LD50 value was considered to be223 mg/kg bw(95% confidence limits:136-366 mg/kg),when rats were treated with Methyltricaprylyl Ammonium Chloride (5137-55-3)orally.

The above study is supported by another experimental study mentioned in authoritative database (201) and secondary source (2018,1992) for thestructurally similar read across substancewas designed and conducted for acute oral toxicity.Acute oral toxicity study was done in 6 male mice using test material Methyltricaprylyl Ammonium Chloride (5137-55-3).Peanut oil was used as vehicle.50% Mortality was observed at dose280 mg/kg bw.Dominant Clinical signs at lethal and nonlethal dosages were Muscle weakness and nervousness.Hence,LD50 value was considered to be 280 mg/kg bw(95% confidence limits:183-430 mg/kg),when mice were treated with Methyltricaprylyl Ammonium Chloride (5137-55-3)orally.

Both these experimental studies are again supported with the structurally similar read across substance.Acute oral toxicity study was done in rats using test chemical.50% mortality was observed at range of 250 mg/kg bw – 300 mg/kg bw. Hence,LD50 value was considered in the range of 250 mg/kg bw – 300 mg/kg bw,when rats were treated with test chemical orally.

Thus, based on the above studies on Methyltricaprylyl Ammonium Chloride (5137-55-3) and it’s structurally similar read across substances, it can be concluded that LD50 value is between 50-300 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, Methyltricaprylyl Ammonium Chloride (5137-55-3) can be classified as category III of acute oral toxicity.

Acute Inhalation Toxicity: 

Methyltricaprylyl Ammonium Chloride (5137-55-3) has very low vapor pressure (2.5E-010 Pa.).So the potential for the generation of inhalable vapours is very low. Also the normal conditions of use of this substance will not result in aerosols, particles or droplets of an inhalable size, so exposure to humans via the inhalatory route will be highly unlikely and therefore the acute inhalation toxicity end point was considered for waiver.

Acute Dermal Toxicity:

Data available for the structurally similar read across chemicals has been reviewed to determine the Acute dermal toxicity of the test chemical methyl(trioctyl)azanium chloride (CAS no.:5137-55-3).The studies are as mentioned below:

1.In acute dermal toxicity study,group of 3 male and female New white Zealand rabbits were treated with test chemical in the concentration of 4300 mg/kg bw by dermal application.The test site (approximately 25% of the total body surface) was clipped with on Oster small animal clipper 24 hours prior to application of the test material. The skin of one male and two females was abraded with the clipper head so as to penetrate the horny layer of the epidermis without causing bleeding. The skin of the remaining three rabbits was left intact.The entire site wascovered with a layer of 8-ply gauze, occluded with rubber dental dam and fastened with Johnson and Johnson Zanas porous tape.Each rabbit was restrained in a Newmann harness end returned to their cage for a 24-hour exposure period. At the end of the exposure period, the harness was removed from each rabbit, the occlusive wrap was removed, and any unabsorbed test material was wiped off with a wet disposable paper towel.Each rabbit was examined thoroughly for signs of systemic toxicity, changes in behavior, mortality and dermal irritation for 14 consecutive days foIIowing the day of dosage.After the 14-day observation period, the surviving animals were weighed, killed by an overdose of Beuthanasia D and necropsied to observe any internal gross effects. A gross necropsy was performed on each animal that died.Three of the six rabbits died during the study.At the end of the 24-hour exposure period all animals exhibited normal behavior and appearance.On the 3rd day ,all rabbits exhibited depressed reflexes, body cold to touch, discharge from the eyes and nose, reddening of the eyes, eating and defecating very little or none at all,and clear fluid around the nose, mouth, chin and front limbs.Rabbit Nos. 1143-975M and 1143-979F held their heads in a downward and tilting position and the nictitating membranes and eyelids appeared reddened. These signs persisted throughout the major portion of the study or until death occurred. Signs of skin irritation noted at the end of the 24-hour exposure period included slight to severe erythema, moderate or severe edema and whitening of the skin of the exposure area.The erythema remained relatively unchanged throughout the study while the edema subsided slightly. Also, moderate or severe atonia was noted on Day 3 through termination or until death Occurred, moderate or marked coriaceous skin from Day 2 through termination or until death in all rabbits; and fissuring in three rabbits and desquamation in one animal. All rabbits showed a substantial weight loss during the study. Necropsy findings in the animals which died included brown, liquid, fecal material around the anal area, bock legs and in colon, lungs adhered to chest wall, lungs white filled with white granular pockets and 9011 bladder enlarged in Rabbit No. 1143-90M; brownish or clear fluid around nose, mouth, chin and front limbs and no other visible lesions in Rabbit Nos. 1143-975M, and 1143-979F.At termination no visible lesion were noted when necropsies were performed on the surviving rabbits.Therefore, LD50 value was considered to be 4300 mg/kg bw,when rabbits were treated with test chemical by dermal applicationfollowing 14 days of observation period.

2.The acute dermal toxicity profile of test chemical in Sprague Dawley rats.The test item was applied to shorn skin of 5 male and 5 female animals at 2000 mg/kg body weight. Administration of the test item at 2000 mg/kg did not result in any skin reaction at the site of application during the study period of 14 days. Administration of the test item did not result in any signs of toxicity and mortality during the study period of 14 days.Animals exhibited normal body weight gain through the study period of 14 days.Gross pathological examination did not reveal any abnormalities attributable to the treatment.Hence, The acute dermal median lethal dose (LD50) of test chemical,when semiocclusively administered to male and female Sprague Dawley rats was considered to be >2000 mg/kg body weight by dermal application following 14 days of observation period according to OECD Guideline 402 (Acute Dermal Toxicity). 

Thus, based on the above summarised studies, methyl(trioctyl)azanium chloride (CAS no.:5137-55-3) and it’s structurally similar read across substances, it can be concluded that LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation,methyl(trioctyl)azanium chloride (CAS no.:5137-55-3)cannot be classified for acute dermal toxicity.

 

Justification for classification or non-classification

Based on the above studies on Methyltricaprylyl Ammonium Chloride (5137-55-3) and it’s structurally similar read across substances, it can be concluded that LD50 value is between 50-300 mg/kg bw for acute oral and >2000 mg/kg bw for acute dermal toxicity. Thus, comparing this value with the criteria of CLP regulation, Methyltricaprylyl Ammonium Chloride (5137-55-3) can be classified as category III for acute oral and cannot be classified for dermal toxicity. For Acute inhalation toxicity wavier was added so, not possible to classify.