Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 248-655-3 | CAS number: 27776-21-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10.11.-14.11.14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study according OECD guideline under GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), Berlin, Germany
- Age at study initiation (mean and range, SD): 2 to 24 h old daphnids were used
- Weight at study initiation (mean and range, SD): -
- Length at study initiation (length definition, mean, range and SD): -
- Valve height at study initiation, for shell deposition study (mean and range, SD): -
- Peripheral shell growth removed prior to test initiation: -
- Method of breeding:
- Feeding during test
- Food type: no feeding
- Amount: no feeding
- Frequency: no feeding
ACCLIMATION
- Acclimation period: not necessary, as test was performed in culture medium
- Acclimation conditions (same as test or not): -
- Type and amount of food: -
- Feeding frequency: -
- Health during acclimation (any mortality observed): - - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 18-22 °C, constant within ± 1 °C
- Nominal and measured concentrations:
- 12.5 - 25.0 - 50.0 - 100.0 - 200.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers, 50 mL
- Type (delete if not applicable): closed (with watch glasses)
- Material, size, headspace, fill volume: glass, 50 mL, 20 mL fill volume
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): renewed after 24 h; new set of test vessels and transfer of daphnids by a pipette
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): -
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium, hardness modified to 160 to 180 mg CaCO3/L
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity: darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (purity: 99.9 %, Sigma)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 87.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 29.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Mortality:
- EC50/LC50: 1.57 (1.53-1.60) mg/L
- Other: - Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the nominal concentrations of the test item Azo Initiator VA044, the 48h-EC50 for Daphnia magna was 87.1 mg/L (95 % confidence limits: 70.7 - 106 mg/L).
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item Azo Initiator VA044 (batch no: N10415) were determined according OECD 202 (2004), equivalent to the Council Regulation (EC) No 440/2008 Method C.2 (2008) from 2014 -11 -12 to 2014 -11 -14 at Dr. U.Noack-Laboratorien, Käthe-Paulus-Str. 1, D-31157 Sarstedt, Germany.
The study was conducted under semi-static conditions over a period of 48 h with 5 concentrations of the test item in the range of 12.5 to 200 mg/L, prepared with dilution water in a geometric series with a seperation factor of 2.
The test item is a white powder and is very soluble in water. As the test item is a light-sensitive substance, the study was performed in darkness. The stock solution of 200 mg/L was freshly prepared with dilution water before the start of both exposure intervals (at 0 and 24 h). Further test concentrations were prepared by diluting the stock solution of 200 mg/L with dilution water. The tested solutions were visually clear throughout the exposure period. Twenty Daphnids were exposed to each concentration level and control.
The concentrations of the test item were analytically verified via LC-MS/MS at the start of the exposure intervals (0 and 24 h) and at the end of the exposure intervals (24 and 48 h) in all concentration levels and the control. The measured concentrations of the test item at the start of the expoure intervals were in the range of 108 to 120 % of the nominal values. At the end of the exposure intervals the concentrations were in the range of 98 to 110 % of the nominal concentrations. These findings indicate that the test item concentrations were successfully maintained for the duration of the experiment.
The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start and the end of the exposure intervals were within the acceptable limits. The validity criteria of the test guidelines were fulfilled.
Reference
Description of key information
Based on the findings in the available OECD 202 study exposing Daphnia magna with the substance an EC50 value of 87.1 mg/L is identified. This value is used for the further chemical safety assessment.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 87.1 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.