Registration Dossier
Registration Dossier
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reaction mass of: pentasodium bis[6-anilino-3,5'-disulfonatonaphthalene-2-azobenzene-1,2'-diolato]cobaltate(III);tetrasodium [6-anilino-3,5'-disulfonatonaphthalene-2-azobenzene-1,2'-diolato][6-anilino-5'-sulfamoyl-3-sulfonatonaphthalene-2-azobenzene-1,2'-diolato]cobaltate(III);trisodium bis[6-anilino-5'-sulfamoyl-3-sulfonatonaphthalene-2-azobenzene-1,2'-diolato]cobaltate(III)
EC number: 444-290-0 | CAS number: 508202-43-5
Endpoint summary
Administrative data
Description of key information
Short-term toxicity to fish:
LC50 (96 h) = 120 mg/l
Short-term toxicity to aquatic invertebrates:
EC50 (48 h) = 27 mg/l
Toxicity to aquatic algae:
ErC50 > 42.6 mg/l
Toxicity to microorganisms:
IC 50 (3 h) >1000 mg/l
Additional information
It is worth saying that the substance is very water soluble (i.e. > 10 g/l); it has a log Kow lower than 4 (indicating a low potential for bioaccumulation) and is not rapidly degradable.
The short-term toxicity to fish was investigated to Oncorhynchus mykiss, according to the OECD Guideline 203 (1992) and the method C.1 of EEC Directive 92/69 EEC. The LC50 (96h) and NOEC were found to be >120 mg/l and 7.5 mg/l, respectively.
The short-term toxicity to invertebrates was investigated to Daphnia magna, according to the OECD Guideline 202, Part I (1984) and to the method C.2 of the EEC-Directive 92/69. The EC50 (48 h) was found to be 27 mg/l.
Toxicity to aquatic algae was investigated to Scenedesmus subspicatus, according to the OECD Guideline 201 (1984) and the method C.3 of EEC-Directive 92/69 EEC. The test method was modified to quantify the algicidal effect of the test item, but also the growth inhibition effect caused by reduced light intensities in the coloured test solutions. The ErC50 (72 h) for algicidal effect was found to be 42.6 mg/l.
Furthermore, microorganisms (activated domestic water sludge) was investigated. The EC50 (3h) was found to be higher than 1000 mg/l.
Justification for classification or non-classification
Adequate chronic toxicity data are not available and the substance is not rapidly degradable; therefore the aquatic toxicity assessment and classification are based mainly on the short-term toxicity data available on the substance. According to the CLP Regulation (EC 1272/2008), acute aquatic toxicity is normally determined using a fish 96-hour LC50, a crustacean species 48-hour EC50 and/or an algal species 72- or 96-hour EC50.
The acute toxicity tests to invertebrates and to aquatic algae fixed the effect levels within the range > 10 to ≤ 100 mg/l.
Therefore, the substance is classified as Category Chronic 3 according to the CLP Regulation (EC 1272/2008).
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