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EC number: 205-622-8 | CAS number: 144-29-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard test guidelines
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- no
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Piperazine citrate / tripiperazine dicitrate- Molecular formula: C12H30N6•Cl2H16O14 - Molecular weight: 642.76 g/mole - Substance type: Organic - Physical state: Solid
- Analytical monitoring:
- not specified
- Details on sampling:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM- Common name:Zebra Fish - Strain:Danio rerio- Source: Unique Aqua Fish Corner- Age at study initiation (mean and range, SD):not specified- Length at study initiation (length definition, mean, range and SD): average 0.180 g- Weight at study initiation (mean and range, SD): average 0.180 g- Method of breeding: not specified- Feeding during test: Once in a day up to sixth day of acclimatization- Food type:Standard brand feed,- Amount: not specified- Frequency: once daily
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- not specified
- Test temperature:
- 23 °C
- pH:
- 7.98
- Dissolved oxygen:
- 7.4 mg/L
- Salinity:
- not specified
- Conductivity:
- not specified
- Nominal and measured concentrations:
- 100 mg/L
- Details on test conditions:
- Photoperiod: 12 hours light and 12-hour darknessVolume of water: 2 litersNo. of animals: 8 fishesTest temperature: 23 °CAdaptation: Test conditions corresponded to housing conditionsTest datesTest started at : 05/03/2016Test Exposure date: 14/03/2016Date of experimental termination: 18/03/2016Withdrawal of food: 1 day before and during exposureAeration: Aeration in test vessels was provided 1 day before the start of experiment
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Observations:
Mortality and symptoms
NominalConcentration
(mg/l)
Number of fish
Mortality (cumulated) after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
-
-
-
-
-
-
-
-
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Visible Symptoms:
NominalConcentration
(mg/l)
Number of fish
Symptoms after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
100
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
100
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
Explanation of symptoms:
A:apathy
N:Narcotic-like state
C:Swimming near water surface
M: Slow movements compared to control
NS:No abnormal symptoms
S:Surfacing behaviour
L:Loss of equilibrium
SM:Shimming movement
H1:Hyperactivity
H2:Hypo activity
B:Bending behavior
F:Fin rot
IS:Invert Swimming
SB:Swimming behaviour
RF:Respiratory Function
P:Pigmentation
pH and oxygen content:
NominalConcentration
(mg/l)
Number of fish
pH readings after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
8.11
8.12
8.12
8.13
8.12
8.14
8.17
8.18
100
4
4
8.24
8.25
8.41
8.42
8.17
8.21
8.24
8.25
100
4
4
8.17
8.19
8.51
8.52
8.19
8.22
8.51
8.51
NominalConcentration
(mg/l)
Number of fish
Oxygen content (mg/L) after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
6.9
6.9
7.1
7.1
7.1
7.1
7.1
7.1
100
4
4
7.2
7.1
7.2
7.2
7.3
7.3
7.3
7.3
100
4
4
7.4
7.4
7.4
7.4
7.1
7.1
7.3
7.3
Test temperature
NominalConcentration
(mg/l)
Number of fish
Temperature (°C) after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
23
23
27
27
26
26
25
25
100
4
4
25
25
26
26
25
26
25
25
100
4
4
25
25
26
26
26
26
25
25
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentration, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. -144-29-6 on Zebra fish (Danio rerio) was determined to be >100mg/L.
- Executive summary:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for the test substance Tripiperazine dicitrate (CAS 144 -29 -6). The nominal concentration selected for the experiment was 100mg/L and Zebra Fish (Danio rerio) were exposed to thisconcentration for 96 hours.
The lethal concentrations LC50 was found to be >100mg/L.
Reference
Description of key information
Based on nominal concentration, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. -144-29-6 on Zebra fish (Danio rerio) was determined to be >100 mg/L. Based on this result it is concluded that the substance Piperazine citrate (CAS no. 144-29-6) does not qualify for the aquatic classification as per the CLP criteria.
For chemical safety assessment key value is considered to be 101 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 101 mg/L
Additional information
Short term toxicity to fish was assessed for the target compound Piperazine citrate (CAS no. 144-29-6) and its related read- across by reviewing a number of studies. The summary of the results are presented below:
In key study, Fish Acute Toxicity test according to OECD Guideline 203 was conducted for the test substanceTripiperazine dicitrate (CAS 144 -29 -6). The nominal concentration selected for the experiment was 100mg/L and Zebra Fish (Danio rerio) were exposed to thisconcentration for 96 hours.
The lethal concentrations LC50 was found to be >100mg/L.
Supporting study from reviewed journal DISEASES OF AQUATIC ORGANISMS, Vol. 10: 39-43, 1991 indicate that the in – vivo toxicity study, Gyrodactylus sp. infecting rainbow trout Oncorhynchus mykiss treated with Piperazine citrate in the concentration of 0 and 200 mg/l. No effect were observed on Percentage of reduction and number of dead (immobile) rainbow trout Oncorhynchus mykiss fish. Therefore, EC0 was considered to be 200 mg/l when Gyrodactylus sp. infecting rainbow trout Oncorhynchus mykiss treated with Piperazine citrate.
Whereas read across substance Piperazine (Cas no. 110-85-0) from Data bank of Environmental Properties of Chemicals (EnviChem), 2016 indicate that Fish toxicity study was conducted for 48 hrs for evaluating the lethal concentration (LC50)of test substance Piperazine on fish Oryzias latipes on the basis of mortality effect. During experiment the lethal concentration (LC50) for test substance Piperazine was found to be >1000 mg/l.
Same read across from European Union Risk Assessment Report – PIPERAZINE, 3rd Priority List Volume: 56;Final Report, 2005 the test chemical Piperazine (Cas no. 110-85-0) indicate semi-static test according to OECD Guidelines 203 (Balk and Meuwsen, 1989b). The test medium was renewed after 48 hours. The nominal test concentrations were 180, 320, 560,1,000 and 1,800 mg/L. Test temperature was 22.3-23°C, pH of the test medium was neutralised to 7.0-7.3. Observations of mortality and sublethal effects among the fish (10 per test concentration) were performed at daily intervals during the test. LC50 could be determined to be>1, 800 mg/L.
Based on above results for target chemical and its read across it is concluded that the substance Piperazine citrate (CAS no. 144-29-6) does not qualify for the aquatic classification as per the CLP criteria.
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