Registration Dossier
Registration Dossier
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EC number: 230-086-7 | CAS number: 6940-53-0
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988-02-04 to 1988-02-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented GLP and guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- GLP compliance statement attached to full study report.
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 1-chloro-2,5-dimethoxy-4-nitrobenzene
- EC Number:
- 230-086-7
- EC Name:
- 1-chloro-2,5-dimethoxy-4-nitrobenzene
- Cas Number:
- 6940-53-0
- Molecular formula:
- C8H8ClNO4
- IUPAC Name:
- 1-chloro-2,5-dimethoxy-4-nitrobenzene
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- - Strain: Hoe: WISKf(SPF71)
- Source: Hoechst AG, kastengrund, SPF breed
- Weight at study initiation:
male: mean = 207 +/- 4 g (min: 202 g, max: 211 g)
female: mean = 204 +/- 4 g (min: 200 g, max: 209 g)
- Age at study initiation: male and females: approx. 9 weeks
- Randomisation according to procedure Nr. 36/88 and 61/88
- Housing: single cages in air conditioned rooms
- Temperature: 22 +/- 3 °C
- Rel. humidity: 50 +/- 20 %
- Lighting: 12 hrs daily
- Acclimatisation period: min 5 days
- Diet: rat diet Altromin 1324, ad libitum
- Water: tap water, ad libitum
- Identification: numbering of cages
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- polyethylene glycol
- Details on dermal exposure:
- - Shaved area: 30 cm^2 mechanically depilated
- Exposed area:the test item with vehicle was spread on an aluminium foil of 6x8 cm
- After 24 hrs of exposure washing with luke warm water - Duration of exposure:
- 24 hrs
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Observation time: 14 days
- Weekly weighting of animals
- Sacrifice and necropsy of animals after observation time.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Mortality:
- None.
- Clinical signs:
- other: None.
- Gross pathology:
- None.
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Executive summary:
1-chloro-2,5-dimethoxy-4-nitrobenzene was tested for its acute dermal toxicity using male and female Wistar rats. After application of a dose of 2000 mg/kg body weight (the limit dose specified in OECD 402) no mortalities or any clinical signs were observed. Necropsy after sacrifice did not reveal any macroscopically visible changes. According to classification criteria as laid down in 83/467(EEC 1-chloro-2,5-dimethoxy-4-nitrobenzene is not classified.
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