Sebacic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

- The test substance was added direct into the study beaker.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Supplier: laboratory breeding
- Feeding: three times a week with green algae (Selenastrum capricornutum)
- During the treatment period the animals were not fed
- Light: 12/16 hours of light/dark
- During the test the animals were kept in the dark

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

251 mg/L of CaCO3

Test temperature:

22°C

pH:

6.5-77

Dissolved oxygen:

7.0-8.0 mg/L

Nominal and measured concentrations:

-Test concentration nominal: 100 mg/L

Details on test conditions:

TEST CONDITIONS
- Test vessels: silylated glass beakers
- 20 animals / concentration
- One group of 20 animals was treated only with reconstituted water
- Age at the beginning of test < 24 hours

TEST MEDIUM / WATER PARAMETERS
- Dilution water according to ISO 6341 - 1982
- sum of calcium and magnesium ions: 25 mmol/L
- Ca/Mg ratio 4/1
- Na/K ratio 10/1

EFFECT PARAMETERS MEASURED
- Observation of immobilization at 24 and 48 hours.
- Parameter checking of dissolved oxygen, pH and temperature at 0 and 48 hours

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No animals were immobilized in the control group and in the treated one.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

other: ISO 14669-1999: Water quallty - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

A substance solubility test was carried out by stirring a concentration of 3200 mg/L test substance in sea w ater, followed by standstill for 4 hours. B y this time it was not dissolved, and further testing was therefore carried out as Water accommodated fractions( WAF).
Water accommodated fractions( WAF) were prepared individually by stirring appropriate amounts of the test substance in growth medium (according to ISO I442:2006). The solutions were stirred with a spin-bar for 22 hours with a speed which formed a vortex one-third of the depth of the fluid content, followed by standstill for about 2 hours. Closed aspirator bottles were used, and samples for testing were drawn off through a drain port near the bottom of the bottle. Test results relate to this water accommodated fraction.

Test organisms (species):

other: Acartia tonsa

Details on test organisms:

- Acartia tonsa was bought as eggs from Marin Biologisk laboratorium, Kobenhavn University, Helsingor.
- The eggs w ere transported by car and airplane to Eurofins on 07.10.2009.
- The test organisms used in the test were hatched 13.10.2009 and held at a temperature of 20+/-3 °C.
- The test organisms were fed with a culture of Rhodomonas baltica from day 2.
- At test start the test organisms were 28 days (after hatching).

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

18.9-20.8 °C

pH:

4.6-8.3

Dissolved oxygen:

Initial: 7.2 - 7.5 mg/L
after 48 hours: 6.2-7.7 (Only for one test concentration the oxygen content was 0.6 mg/L. Hence for this test vessel the acceptance criteria are not fulfilled)

Salinity:

34.4 per mill

Nominal and measured concentrations:

-Test concentrations nominal: 3, 10, 32, 100, 320 and 1010 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessels: glass containers (28 mL rolled rim vials) each containing 25 mL of test solution.
- Six concentrations of the test substance were tested in a geometric series.
- Four replicates were prepared for each concentration.
- Six replicates were prepared for the blank and for the fererence substance.
- The vials were kept under a 16h/8h light/dark photoperiod.

TEST MEDIUM / WATER PARAMETERS
- Natural seawater from an unpolluted site at about 80 m depths in Byfjord was collected from a tap.
- The seawater was filtered through a GF/C filter and briefly heated to about 75 °C prior to use.
- Seawater with addition of nutrient (accordimg to ISO 110253:1998) was used scince an algae test also was conducted.

EFFECT PARAMETERS MEASURED
- Lethality was registered after 24 and 48 hours by visual observation.
- Measurement of oxygen and pH were done at start and after 48 hours.

Reference substance (positive control):

yes

Remarks:

3,5-dichlorphenol

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

18 mg/L

Nominal / measured:

nominal

Conc. based on:

other: WAF (Water accommodated fraction)

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

other: WAF

Details on results:

Due to the fact that insufficient data were available for statistical evaluation, the LC50 values w ere calculated by interpolation.
Calculation of NOEC is done by single-factor ANOVA followed by Dunnetts procedure for comparing each concentation mean w ith the control mean.

Description of key information

Sebacic acid is acutely harmful to aquatic invertebrates.

Additional information

The acute toxicity of sebacic acid was studied with the marine copepod Acartia tonsa, in accordance to ISO 14669 [BASF SE, 2009]. The interpolated 48 hours LC50 value is 18 mg/L WAF (water accommodated fraction).

Additional the acute toxicity of sebacic acid was tested with the freshwater organism Daphnia magna, in accordance to OECD 202 [Ausimont S.p.A., 1999]. In this limit test with a nominal test concentration of 100 mg/L no immobilisation of the water fleas were observed.