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Toxicity to microorganisms

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Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
GLP conform OECD 301B biodegradation test, which in general do not focus on the hazard assessment towards microorganisms. However, the degradation of the inhibition control can be considered as reliable information about the toxicity of the test substance to aquatic microorganisms.
Qualifier:
according to
Guideline:
other: OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
Activated sludge from laboratory wastewater plant treating municipal sewage.
Concentration of activated sludge in the test assay: 30 mg/L dry substance
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
28 d
Test temperature:
22 +/- 2°C
pH:
7.2 - 7.8
Nominal and measured concentrations:
Test concentration (nominal): 37.2 mg/L
Details on test conditions:
for details see chapter 5.2.1
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
37.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: biodegradation of aniline

- Degradation degree in the inhibition control after 14 days: 70-80 %

Conclusions:
The test substance does not inhibit activated sludge at a concentration of 37.2 mg/L.
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure in accordance with German national standard; no GLP
Qualifier:
according to
Guideline:
other: DIN 38412, Part27; Pseudomonas Respiration Inhibition Test
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
stock solution with 100 mg/L Tween 80
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min
Test temperature:
20 - 21°C
pH:
7.8 - 8.0
Nominal and measured concentrations:
Test concentrations (nominal): 31.3, 62.5, 125, 250, 500 and 1000 mg/L
Key result
Duration:
30 min
Dose descriptor:
EC10
Effect conc.:
280 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
443 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
other: EC90
Effect conc.:
875 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Validity criteria fulfilled:
not specified
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge, industrial
Details on inoculum:
sludge from BASF waste water treatment plant
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min
Key result
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
390 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Description of key information

The inhibition of the degradation activity of activated sludge is not anticipated when triisobutyl phosphate introduced in appropriate low concentrations.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
37.2 mg/L

Additional information

No microorganismen respiration inhibition test according to OECD guideline 209 is available. However, the toxicity of Triisobutyl phosphate (TriBP) was assessed based on the inhibition control of a ready biodegradation test performed according to GLP and OECD guideline 301B (BASF 2004). 37.2 mg/L test substance and aniline was mixed. No inhibition of aniline degradation was observed throughout an exposure time of 28 days. Thus the 28d-NOEC value is determined to be 37.2 mg/L. Additional data from a robra test with Pseudomonas putida (30 min-EC10 = 280 mg/L); BASF 1989) and a respiration inhibition test with activated sludge from an industrial STP (30 min-EC20 = 390 mg/L; BASF 1984) confirm the conservative assumption of no toxicity below 37.2 mg/L.