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EC number: 932-121-8 | CAS number: 1147459-12-8
Biodegradation in water and sediment: simulation tests
Administrative data
Link to relevant study record(s)
Description of key information
These results as observed for Amides, C18-unsatd., N-[3-(dimethylamine)propyl] can be considered to represent the worst-case removal for N-[3-(dimethylamino)propyl]-C12-18(even numbered)-alkylamide. Indeed, as N-[3-(dimethylamino)propyl]-C12-18(even numbered)-alkylamide has a smaller carbon chain length, a similar or higher removal is expected because of the higher bioavailability for biodegradation. It is confirmed by the high percentage of biodegradation (85%) observed for N-[3-(dimethylamino)propyl]-C12-18(even numbered)-alkylamide in a biodegradation screening test (OECD 301D).
And N-[3-(dimethylamino)propyl]-C12-18(even numbered)-alkylamide is expected to be removed to a lower extend via sorption compared to Amides, C18-unsatd., N-[3-(dimethylamine)propyl] to N-[3-(dimethylamino)propyl]-C12-18(even numbered)-alkylamide.
It is therefore considered justified to use the results of this CAS test obtained with Amides, C18-unsatd., N-[3-(dimethylamine)propyl] to N-[3-(dimethylamino)propyl]-C12-18(even numbered)-alkylamide.
Key value for chemical safety assessment
Additional information
The removal of Amides, C18-unsatd., N-[3-(dimethylamine) propyl] was simulated in a continuous activated sludge (CAS) test unit performed according to ISO Guidelines, and in compliance with the OECD principles of GLP (Van Ginkel, 2010). Amides, C18-unsatd., N-[3-(dimethylamine) propyl] were exposed to micro-organisms maintained by addition of domestic wastewater in the CAS test. The test substance was spiked at a nominal influent concentration of 50 mg/L (36.5 mg/L carbon; calculated) for a period of 48 days and included a control fed with domestic wastewater only. The immediate high removal percentages can be attributed to adsorption and probably biodegradation.
The mean removal percentage of test substance calculated over 14 measurements obtained from day 35 to 48 of the test was 105 +/-1% (95% confidence interval). These high removal percentages strongly indicate that the test substance is biodegraded ultimately. Formation of water-soluble compounds during biological treatment of Amides, C18-unsatd., N-[3-(dimethylamine) propyl] can be excluded.
An accurate assessment of the removal of Amides, C18-unsatd., N-[3-(dimethylamine) propyl] was established with specific analyses. The method (LC-MS/ MS) for the determination of Amides, C18-unsatd., N-[3-(dimethylamine) propyl] was satisfactory with regard to the linearity, repeatability of the injections, limit of quantification (LOQ), recovery and specificity. The mean removal percentage of test substance in the test unit was assessed with the specific analysis from day 44 to 48 was >99.997% using octadecenamide-N- [3-(dimethylamino) propyl] as representative component. These analyses demonstrate that the removal of test substance is almost complete. Concentrations in the sludge of the reactor sampled on days 47 and 48 were 341 and 24 µg/L. Mean removal percentages of Amides, C18-unsatd., N-[3-(dimethylamine) propyl] from the influent through adsorption onto sludge assessed in two samples was therefore <0.02% demonstrating that the test substance is primarily removed by biodegradation. In conclusion, the CAS test demonstrates that Amides, C18-unsatd., N-[3-(dimethylamine) propyl] is almost completely removed from the wastewater in conventional biological wastewater treatment plants. Amides, C18-unsatd., N-[3-(dimethylamine) propyl] is primarily removed by biodegradation.
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