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EC number: 210-765-4 | CAS number: 623-03-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
A study on the acute oral toxicty of 4-Chlorobenzonitrile was performed according to OECD 423. In conclusion,
the LD50 of 4-Chlorobenzonitrile is between 300 and 2000 mg/kg bodyweight by oral route in the rat. In accordance with the OECD 423 (Annex 2d),
the LD50 cut-off of the test item may be considered to be 500 mg/kg body
weight by oral route in the rat.
The LD50 value was also extracted from the manufacturers’ safety data
sheet. Additional short-term data is presented in Section 7.9.3. An LD50
> 300mg/kg was confirmed (Heilmann, 1978). A single i.p. injection of
1.5 mmol/kg was reported not leading to hepatotoxic effects (Hanzlik,
1978).
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04.02.2014 - 21.02.2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 tris (Acute Oral Toxicity - Acute Toxic Class Method)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- no
- Species:
- rat
- Strain:
- other: Crl:(WI)BR
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: TOXI COOP ZRT. Cserkesz u. 90., 1103 Budapest, Hungary
- Age at study initiation: 10 weeks
- Weight at study initiation: 200-220 g
- Fasting period before study: The day before treatment the animals were fasted. The food but not water was withheld overnight.
- Housing: Group caging (3 animals/cage), Type II polypropylene/polycarbonate
- Diet (e.g. ad libitum): ad libitum, ssniff SM R/M-Z+H complete diet for rats and mice produced by ssniff Spezialdiäten GmbH, 59494 Soest, Germany
- Water (e.g. ad libitum): tap water from municipal supply as for human consumption from bottle ad libitum
- Acclimation period: 19 days in first step, 20 days in second step and 21 days in third step
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3 °C
- Humidity (%): 30-70 %
- Air changes (per hr): 10-15 air changes/hour by central air-condition system
- Photoperiod: artificial light from 6 a.am to 6 p.m. - Route of administration:
- oral: gavage
- Vehicle:
- other: 1.0 % Methyl Cellulose (Methylcellulosum/Aqua purificata)
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 200 and 30 mg/L
- Lot/batch no. (if required): Methylcellulosum: N83746634; Aqua purificata: 1311-5507
MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg bw
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: Selection on the basis of the available information about the test item - Doses:
- 2000 and 300 mg/kg bw
- No. of animals per sex per dose:
- 3 females (nulliparous and non pregnant) per dose
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Mortality: 30 min, 1 h, 2 h, 4 h after the treatment and twice each day for 14 days thereafter; General state, external appearance, behavior and clinical symptoms: at least once during the first 30 min, then 1 h, 2 h, 3 h, 4 h after treamtment and once each day for 14 days thereafter; Body weights: recorded on day 0 (just before the treament), on day 7 and on day 15.
- Necropsy of survivors performed: yes - Statistics:
- no data
- Preliminary study:
- not applicable
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 300 - < 2 000
- Based on:
- test mat.
- Remarks on result:
- other: The Method according to OECD 423 is not intended for the precise calculation of a precise LD50 value.
- Mortality:
- All animals treated with 2000 mg/kg bw single oral dose of the test item 4-Chlorobenzonitrile were sacrificed as moribund on Day 1.
No death occurred at 300 mg/kg bw single oral dose of the test item. All female rats in step 2 and step 3 survived until the end of the 14-day observation period. - Clinical signs:
- other: In group 1 treated with 2000 mg/kg bw dose clinical sign of reaction comprised of decreased activity (18 cases of 18 observations), clonic convulsion (3/18), abnormal gait (12/18), prostration (3/18), crouching (9/18), incoordination (6/18), decreased rig
- Gross pathology:
- All rats treated with 2000 mg/kg bw dose of the test item were sacrificed as moribund on Day 1. All animals of the 300 mg/kg bw dose survived until the scheduled necropsy on Day 15. External necropsy findings as piloerection and lacrimation were detected in all animals of group 1. Internal necropsy finding as pale kidneys was observed in two animals (No.: 9978, 9982) of group 1. This alteration could not be related to the test item toxic effect, but was regarded an individual variation. Most likely the observation is a congenital anomaly. Slight hydrometra was observed in female No.: 9987 of the group 2 and two females (No: 9977, 9985) of group 2. It is physiological finding and connected to the cycle of the animal.
No macroscopical changes were found related to the effect of the test item during the macroscopic examination of the animals of 300 mg/kg bw dose group. - Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- In conclusion, the LD50 of the test item4-Chlorobenzonitrile is between 300 and 2000 mg/kg bodyweight by oral route in the rat.
In accordance with the OECD Guideline No. 423 (Annex 2d), the LD50 cut-off of the test item may be considered to be 500 mg/kg body weight by oral route in the rat. - Executive summary:
In an acute oral toxicity study according to OECD 423 with 4-Chlorbenzonitrile the starting dose was selected on the basis of the available information about the test item.
The acute toxic class method was carried out involving a stepwise procedure with the use of 2000 mg/kg bw as the starting dose in three female rats. All animals died in step 1, so the test was continued at a lower dose level (300 mg/kg bw) on further three female rats. Since no animal died in step 2, the test was repeated at same dose level (300 mg/kg bw) on further three female rats. There was no death in the third step, too, so the test was finished because the stopping criteria of Annex 2d of OECD Guideline No. 423 was met.
Animals were weighed, observed for lethality and toxic symptoms for 14 days after the treatment. Gross pathological examination was carried out in moribund animals on Day 1, as well as 15th day after the treatment in survivor animals.
In conclusion, the LD50 of 4-Chlorobenzonitrile is between 300 and 2000 mg/kg bodyweight by oral route in the rat. In accordance with the OECD Guideline No. 423 (Annex 2d), the LD50 cut-off of the test item may be considered to be 500 mg/kg body weight by oral route in the rat.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 500 mg/kg bw
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
A study on the acute oral toxicty of 4-Chlorobenzonitrile was performed according to OECD 423. In conclusion, the LD50 of 4-Chlorobenzonitrile is between 300 and 2000 mg/kg bodyweight by oral route in the rat. In accordance with the OECD 423 (Annex 2d), the LD50 cut-off of the test item may be considered to be 500 mg/kg body weight by oral route in the rat.
No data are available on inhalation toxicity.
Justification for classification or non-classification
According to REGULATION (EC) No 1272/2008 a classification as Acute Tox 4 (H302: Harmful if swallowed) is warranted based on an LD50 above 300 mg/kg bw.
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