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EC number: 210-765-4 | CAS number: 623-03-0
Biodegradation in water and sediment: simulation tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: sediment simulation testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: no guideline reported
- Principles of method if other than guideline:
- Sediment samples (solid content: 69 g/L) were incubated at 25 °C under nitrogen atmosphere with 1.4 mg/L = 0.01 mmol/L. In a second experiment with sediment samples having a solid content of 93 mg/l, 4-chlorobenzonitrile was incubated at an initial concentration of 0.14 mg/L.
In a third experiment sediment extracts were prepared by extracting sediment samples with 20 % glycerin. The protein fraction was precipitated using gel chromatography and ion-exchange chromatography. The benzonitrile metabolising portein fraction was used in the degradation experiments with 4-chlorobenzonitrile at a concentration of 1.4 mg/L = 0.01 mmol/L. - GLP compliance:
- not specified
- Radiolabelling:
- no
- Oxygen conditions:
- anaerobic
- Inoculum or test system:
- natural sediment
- Duration of test (contact time):
- >= 40 d
- Initial conc.:
- 1.4 mg/L
- Based on:
- test mat.
- % Degr.:
- 45
- Parameter:
- test mat. analysis
- Sampling time:
- 20 d
- Remarks on result:
- other: in sediment samples, solid content: 69 mg/L; no lag phase; first order reaction
- % Degr.:
- 70
- Parameter:
- test mat. analysis
- Sampling time:
- 40 d
- Remarks on result:
- other: in sediment samples, solid content: 69 mg/L; no lag phase; first order reaction
- % Degr.:
- 100
- Parameter:
- test mat. analysis
- Sampling time:
- 8 d
- Remarks on result:
- other: in sediment extracts; zero order reaction
- Compartment:
- sediment
- DT50:
- 19 d
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: in sediment samples, solid content: 93 mg/L
- Transformation products:
- no
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- In sediment samples with 69 mg solids/L 70 % of the initial test substance has been degraded after 40 days. The degradation experiment with 93 mg/L revealed a degradation half-life of 19 days.
In the experiments using sediment extracts, the test substance 4-chlorobenzonitrile was totally degraded within 8 days. - Executive summary:
Anaerobic sediment samples with a solid content of 69 g/L and 93 mg/L were incubated at 25 °C under nitrogen atmosphere with 1.4 mg/L (= 0.01 mmol/L) and 0.14 mg/L, respectively.
In an other experiment sediment extracts were prepared by extracting sediment samples with 20 % glycerin. The protein fraction was precipitated using gel chromatography and ion-exchange chromatography. The benzonitrile metabolising portein fraction was used in the degradation experiments with 4-chlorobenzonitrile at a concentration of 1.4 mg/L = 0.01 mmol/L.
In sediment sample with 69 g solids/L 70 % of the initial test substance has been degraded after 40 days. The degradation experiment with 93 mg/L revealed a degradation half-life of 19 days.
In the experiments using sediment extracts, the test substance 4-chlorobenzonitrile was totally degraded within 8 days.
Transformation products were not detected in any of the experiments.
- Endpoint:
- biodegradation in water: sediment simulation testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 308 (Aerobic and Anaerobic Transformation in Aquatic Sediment Systems)
- GLP compliance:
- no
- Radiolabelling:
- no
- Oxygen conditions:
- anaerobic
- Inoculum or test system:
- natural sediment
- Duration of test (contact time):
- <= 100 d
- Initial conc.:
- 1 µmol/L
- Based on:
- test mat.
- Compartment:
- sediment
- DT50:
- 18.8 d
- St. dev.:
- 2.2
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: r²=0.949
- Transformation products:
- yes
- No.:
- #1
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The degradation half-life of 4-chlorobenzonitrile in anaerobic sediment slurry is 18.8 (+/- 2.2) d.
- Executive summary:
The degradation of 4-chlorobenzonitrile was investigated in anaerobic sediment systems under laboratory conditions. 4-chlorobenzonitrile was incubated at an initial concentration of 1 µmol/L at 22 °C. The sediment/water ratio of the sediment used was 0.02, the organic carbon content and pH were 32.0 % and 7.3, respectively. Test substance degradation was followed by HPLC and UV measurements.
The degradation half-life of 4-chlorobenzonitrile under the conditions of the test was determined to be 18.8 (+/- 2.2) d.
Referenceopen allclose all
Description of key information
The degradation of 4-chlorobenzonitrile was investigated in anaerobic sediment
systems under laboratory conditions.
The degradation half-life of 4-chlorobenzonitrile under the conditions
of the test was determined to be 18.8 (+/- 2.2) d.
Key value for chemical safety assessment
- Half-life in freshwater sediment:
- 19 d
- at the temperature of:
- 22 °C
Additional information
The degradation of 4-chlorobenzonitrile was investigated in anaerobic sediment systems under laboratory conditions (see "623 -03 -0_4 -Chlorbenzonitril_kinetic_sediment_1992.pdf"). 4-chlorobenzonitrile was incubated at an initial concentration of 1 µmol/L at 22 °C. The sediment/water ratio of the sediment used was 0.02, the organic carbon content and pH were 32.0 % and 7.3, respectively. Test substance degradation was followed by HPLC and UV measurements.
The degradation half-life of 4-chlorobenzonitrile under the conditions of the test was determined to be 18.8 (+/- 2.2) d.
In additional experiments (see "623-03-0_4-Chlorbenzonitril BUA Stoffbericht") anaerobic sediment samples with a solid content of 69 g/L and 93 mg/L were incubated at 25 °C under nitrogen atmosphere with 1.4 mg/L (= 0.01 mmol/L) and 0.14 mg/L, respectively.
In addition an experiment was conducted using sediment extracts. The initial 4-chlorobenzonitrile concentration was 1.4 mg/L.
In sediment sample with 69 g solids/L 70 % of the initial test substance has been degraded after 40 days. The degradation experiment with 93 mg/L revealed a degradation half-life of 19 days.
In the experiments using sediment extracts, the test substance 4-chlorobenzonitrile was totally degraded within 8 days.
Transformation products were not detected in any of the experiments.
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