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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented study but no data on GLP compliance.

Data source

Reference
Reference Type:
publication
Title:
Effect of Microsomal Enzyme Inducing Agents on Hepatic Biotransformation in Cotton Rats (Sigmodon Hispidus): Comparison to that in Sprague-Dawley Rats.
Author:
Watkins JB
Year:
1991
Bibliographic source:
Comparative biochemistry and physiology. C. Comparative pharmacology and toxicology 1991, vol. 98, no2-3, pp. 433-439 [7 page(s) (article)]

Materials and methods

Objective of study:
metabolism
Principles of method if other than guideline:
no data
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-naphthol
EC Number:
205-182-7
EC Name:
2-naphthol
Cas Number:
135-19-3
Molecular formula:
C10H8O
IUPAC Name:
2-naphthol
Details on test material:
"highest quality available"

Test animals

Species:
rat
Strain:
other: Cotton (Sigmodon Hispidus) and Sprague-Dawley rats
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: Saline and corn oil
Details on exposure:
Assays were performed in duplicate or triplicate samples from each animal.
Separate groups of male animals were treated i.p. for four days with either 75 mg phenobarbital/kg in saline, 75 mg pregnenolone-16alpha-carbonitrile/kg suspended in 2% Tween 80 in saline, or 20 mg 2-methylcholanthrene/kg in corn oil.
Control animals received either corn oil or saline.

Duration and frequency of treatment / exposure:
Duration of treatment: 4 days
Doses / concentrations
Remarks:
Doses / Concentrations:
All solutions were administered in a volume of 5 mL/kg.
No. of animals per sex per dose / concentration:
6-8 animals male/ group
Control animals:
yes
Details on dosing and sampling:
All solutions were administered in a volume of 5 mL/kg. 24 hrs after the last dose, the rats were anesthetized with urethane and livers were excised, rinsed in ice-cold KCl, and a 25% homogenate in 0.25M sucrose was prepared (containing 10mM Tris-HCl, pH 7.4, and 3 mM 2-mercaptoethanol).
Statistics:
Means and standard errors were generated for each group, and the data were analyzed by a one-way or two-way analysis of variance as appropriate.Duncan`s new multiple range test was used to compare the means. Significance was set at P < 0.05.

Results and discussion

Toxicokinetic / pharmacokinetic studies

Details on absorption:
Sulfotransferase activity in male Sprague-Dawley rats for 2-naphthol was 0.641 +/- 0.021 nmol/min/mg protein (mean +/- SD for 15 rats). Sulfation in cotton rats was only 15-30% of that observed in SD rats and was not increased by inducer administration.

Applicant's summary and conclusion

Conclusions:
Sulfotransferase activity in male Sprague-Dawley rats for 2-naphthol was 0.641 +/- 0.021 nmol/min/mg protein (mean +/- SD for 15 rats). Sulfation in cotton rats was only 15-30% of that observed in SD rats and was not increased by inducer administration.