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EC number: 229-552-2 | CAS number: 6606-65-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1991
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 798.5265 (The Salmonella typhimurium Bacterial Reverse Mutation Test)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- LID-1187A (n-butylcyanoacrylate)
- IUPAC Name:
- LID-1187A (n-butylcyanoacrylate)
- Reference substance name:
- Enbucrilate
- EC Number:
- 229-552-2
- EC Name:
- Enbucrilate
- Cas Number:
- 6606-65-1
- Molecular formula:
- C8H11NO2
- IUPAC Name:
- butyl 2-cyanoprop-2-enoate
- Test material form:
- other: solution
- Details on test material:
- - LID-1187A (n-butylcyanoacrylate, CAS 6606-65-1, 97% a.i., ref.#1577-19), a pale yellow liquid, contained in white plastic bottles, was received on 17 September 1990. The bottles were individually packed in sterile sachets, also containing tubing and applicator nozzles. It was stored at approximately 4°C (except for a 14-day period at ambient temperature between 24 September and 8 October) and protected from light until required.
- Histoacryl (n-butylcyanoacrylate, CAS 6606-65-1, lot 2/9484), a blue liquid, contained in plastic ampoules within plastic tubes, was received on 15 March 1990. It was stored at approximately 4°C and protected from light until required.
- Solutions, which were freshly prepared immediately before use, were made in acetone. The LID-1187A was passed through the plastic tubing and nozzle provided by means of a peristaltic pump before the preparation of an acetone solution, and both materials were tested within one hour of exposure to air. All concentrations cited in this report refer to the samples as received. No determinations of stability, homogeneity or achieved concentration were undertaken on the materials solutions. Temperature and relative humidity levels were measured and recorded, both in the laboratory and within the safety cabinet in which all the tests were conducted, during all the tests.
Constituent 1
Constituent 2
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: Histidine auxotroph
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 mix
- Test concentrations with justification for top dose:
- 2.5, 5, 25, 50, 250, 500, 2500 and 5000 µg/plate (pre-experiment)
25, 70, 250, 790, 2500 µg/plate (main experiment) - Vehicle / solvent:
- aceton
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- benzo(a)pyrene
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- TEST ORGANISMS:
Cultures of the histidine-dependent strains of Salmonella typhimurium were derived from cultures provided by Prof. Bruce Ames, University of California. The characteristics of the individual strains are as follows:
- TA 1535 - contains a histidine missense mutation but is also deficient in a DNA repair system (uvr B) and has a defective lipopolysaccharide coat on the cell wall. It is reverted by many agents causing base-pair substitutions, but is not sensitive to frameshift mutagens.
- TA 100 - is the same as TA 1535 but contains a resistance transfer factor conferring ampicillin resistance and increasing sensitivity to same mutagens (plasmid pKM 101). In addition to base-pair substitutions, it is also able to detect certain frameshift mutagens.
- TA 1537 - bears a histidine frameshift mutation. Like TA 1535, it is defective in a DNA repair system and lipopolysaccharide coat. It is sensitive to agents causing frameshift mutations involving insertion or deletion of a single base-pair.
- TA 98 - contains another histidine frameshift mutation. Again it has a defective DNA repair system and lipopolysaccharide coat but also contains the pKM 101 plasmid. It is reverted by agents causing deletion of two adjacent base-pairs (double frameshift mutations), but not by simple alkylating agents causing base-pair substitutions.
Cultures of all organisms were prepared by overnight incubation of nutrient broth (Oxoid No.2) freshly inoculated from a frozen culture stock.
HUMIDITY AND TEMPERATURE CONDITIONS: Temperature and humidity levels were recorded both in the laboratory (22°C / 46-64% R.H.) and within the safety cabinet (25-27°C / 32-50% R.H.).
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
RESULTS
PRELIMINARY TOXICITY TESTS
LID-1187A and Histoacryl were tested in concentrations of 2.5, 5, 25, 50, 250, 500, 2500 and 5000 µg/plate. For both test materials, the lowest level to cause visible thinning of the background lawn of non-revertant cells was 2500 µg per plate. This was therefore selected as the top exposure level for use in the main assays.
STERILITY CHECKS, SPONTANEOUS REVERSION RATE AND VIABILITY CHECKS
The absence of colonies on test material and S-9 mix sterility check plates indicates that these preparations were free of significant microbial contamination. The total colony counts on plates containing the 10-6dilution of bacterial culture only confirmed the viability and high cell density of the cultures of the individual organisms. The counts recorded on appropriate negative control plates confirmed the characteristically low spontaneous reversion rates of the tester strains and the absence of effects on these rates of acetone inclusion.
MUTAGENIC ACTIVITY OF POSITIVE CONTROL CHEMICALS
Appropriate positive control chemicals (with S-9 mix where required) induced marked increases in revertant colony numbers with all strains, confirming sensitivity of the cultures and activity of the S-9 mix.
ACTION OF LID-1187A AND HISTOACRYL
No increases in revertant colony numbers over control counts were obtained with any of the tester strains following exposure to either test material at levels from 25 to 2500 µgper plate.
Inhibition of growth, observed as thinning of the background lawn of non-revertant cells and reduction in revertant colony numbers, occurred in all strains following exposure to both test materials at 2500µgper plate.
MEAN COLONY COUNTS FOR LID-1187A
No. |
Addition (µg) |
S-9 |
TA98 |
TA98 |
TA100 |
TA100 |
TA1535 |
TA1535 |
TA1537 |
TA1537 |
1 |
None |
+ |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
LID-1187A (2500), sterility check |
- |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3 |
2500 |
+ |
7 |
16 |
57 |
72 |
5 |
6 |
2 |
6 |
4 |
790 |
+ |
26 |
26 |
106 |
98 |
10 |
8 |
5 |
8 |
5 |
250 |
+ |
32 |
31 |
115 |
105 |
9 |
9 |
6 |
9 |
6 |
79 |
+ |
30 |
30 |
118 |
114 |
11 |
10 |
7 |
8 |
7 |
25 |
+ |
33 |
32 |
116 |
108 |
14 |
14 |
7 |
6 |
8 |
Acetone (0.1 mL) |
+ |
31 |
31 |
117 |
107 |
15 |
13 |
7 |
7 |
9 |
2500 |
- |
14 |
10 |
46 |
60 |
6 |
4 |
1 |
4 |
10 |
790 |
- |
22 |
24 |
100 |
98 |
11 |
8 |
4 |
4 |
11 |
250 |
- |
28 |
29 |
106 |
109 |
10 |
9 |
5 |
6 |
12 |
79 |
- |
28 |
30 |
108 |
111 |
11 |
12 |
5 |
5 |
13 |
25 |
- |
31 |
27 |
112 |
110 |
13 |
12 |
5 |
5 |
14 |
Acetone (0.1 mL) |
- |
32 |
30 |
109 |
107 |
16 |
13 |
5 |
6 |
18 |
None (dilution of bact. culture) |
- |
141 |
125 |
145 |
136 |
127 |
120 |
139 |
123 |
MEAN COLONY COUNTS FOR HISTOACRYL
No. |
Addition (µg) |
S-9 |
TA98 |
TA98 |
TA100 |
TA100 |
TA1535 |
TA1535 |
TA1537 |
TA1537 |
1 |
None |
+ |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
Histoacryl (2500), sterility check |
- |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3 |
2500 |
+ |
10 |
12 |
69 |
55 |
5 |
5 |
2 |
5 |
4 |
790 |
+ |
18 |
24 |
95 |
87 |
12 |
10 |
4 |
7 |
5 |
250 |
+ |
29 |
24 |
105 |
94 |
11 |
8 |
6 |
8 |
6 |
79 |
+ |
29 |
29 |
114 |
104 |
13 |
10 |
6 |
9 |
7 |
25 |
+ |
31 |
30 |
114 |
109 |
8 |
11 |
5 |
7 |
8 |
Acetone (0.1 mL) |
+ |
31 |
31 |
117 |
107 |
15 |
13 |
7 |
7 |
9 |
2500 |
- |
5 |
6 |
32 |
62 |
5 |
7 |
0 |
0 |
10 |
790 |
- |
22 |
23 |
95 |
105 |
10 |
7 |
5 |
3 |
11 |
250 |
- |
27 |
25 |
105 |
105 |
11 |
10 |
4 |
5 |
12 |
79 |
- |
29 |
27 |
105 |
104 |
11 |
12 |
5 |
4 |
13 |
25 |
- |
32 |
25 |
110 |
107 |
12 |
13 |
4 |
6 |
14 |
Acetone (0.1 mL) |
- |
32 |
30 |
109 |
107 |
16 |
13 |
5 |
6 |
18 |
None (dilution of bact. culture) |
- |
141 |
125 |
145 |
136 |
127 |
120 |
139 |
123 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test materials, LID-1187A and Histoacryl, were devoid of mutagenic activity under the conditions of the test. - Executive summary:
SUMMARY
LID-1187A and Histoacryl were examined for mutagenic activity in four histidine-dependent auxotrophs of Salmonella typhimurium, strains TA 98, TA 100, TA 1535 and TA 1537, using pour-plate assays. The procedures used complied with OECD Guideline for Testing of Chemicals No. 471 (issued 1983) and EPA Toxic Substances Control Act Test Guideline § 798.5265 (first issued 1985, amended 1987). Each test, in each strain, was conducted on two separate occasions.
The studies, which were conducted in the absence and presence of an activating system derived from rat liver (S-9 mix), employed a range of levels of both test materials from 25 to 2500 µg per plate, selected following a preliminary toxicity test in strain TA 98. All tests included solvent (acetone) controls with and without S-9 mix.
No increases in reversion to prototrophy were obtained with any of the four bacterial strains following exposure to either LID-1187A or Histoacryl at the levels tested, either in the presence or absence of S-9 mix. Inhibition of growth, observed as thinning of the background lawn of non-revertant cells and reduction in revertant colony numbers, occurred in all strains following exposure to both test materials at 2500 µg per plate.
Marked increases in the number of revertant colonies were induced by the known mutagens benzo[a]pyrene, 2-nitrofluorene, 2-aminoanthracene, 9-aminoacridine and sodium azide when examined under similar conditions.
It was concluded that both LID-1187A and Histoacryl were devoid of mutagenic activity under the conditions of the test.
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